Mouse Periostin/OSF-2 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MOSF20
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.065 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse Periostin/OSF-2 Quantikine ELISA Kit
The Quantikine Mouse Periostin/OSF-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Periostin in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse Periostin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Periostin. Results obtained using natural mouse Periostin showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Periostin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse Periostin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 0.600 | 0.833 | 4.69 | 0.629 | 0.867 | 4.84 |
| Standard Deviation | 0.021 | 0.035 | 0.117 | 0.056 | 0.067 | 0.260 |
| CV% | 3.5 | 4.2 | 2.5 | 8.9 | 7.7 | 5.4 |
Recovery for Mouse Periostin/OSF-2 Quantikine ELISA Kit
The recovery of mouse Periostin spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=6) | 95 | 81-117 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Periostin in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse Periostin/OSF-2 Quantikine ELISA Kit
Mouse Periostin/OSF-2 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Periostin/OSF-2
Periostin, also known as OSF-2, is a secreted matricellular protein with functions in extracellular matrix formation, cell migration, and inflammation. It is secreted as a 90 kDa monomer that can aggregate into >170 kDa higher-order multimers. Periostin is expressed by mesenchymal cells such as vascular smooth muscle cells, fibroblasts, osteoblasts, and odontoblasts in developing teeth. It is upregulated in many carcinomas. Periostin binds to multiple Integrins and enhances cell adhesion and cell migration. It enhances Fibronectin and Collagen I production and promotes collagen fibrillogenesis. It also induces epithelial-mesenchymal transition, tumor growth, invasion, and metastasis. Periostin induces the expression of VEGF R2 on endothelial cells and VEGF-C in tumor cells, and it can induce tumor lymphangiogenesis. Periostin plays an important role in heart valve development and tissue healing after myocardial infarction. In asthma, it is upregulated in bronchial epithelium and plays both destructive and protective roles by inducing eosinophil infiltration and inhibiting goblet cell metaplasia and mucus production.
Long Name
Osteoblast Specific Factor 2
Alternate Names
Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52
Gene Symbol
POSTN
Additional Periostin/OSF-2 Products
Product Documents for Mouse Periostin/OSF-2 Quantikine ELISA Kit
Product Specific Notices for Mouse Periostin/OSF-2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.