Mouse G-CSF DuoSet ELISA

Name: Mouse G-CSF DuoSet ELISA
Brand: R&D Systems
SKU: DY414
Rating: 5 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY414

Citations (25)

(3)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	31.2 - 2,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Mouse G-CSF DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Mouse G-CSF DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse G-CSF. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Mouse G-CSF DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Mouse
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For five and fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Mouse G-CSF DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Mouse G-CSF DuoSet ELISA

	Mouse G-CSF ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: G-CSF

Mouse granulocyte-colony stimulating factor (G-CSF) is a 24-25 kDa monomeric glycoprotein that regulates the proliferation, differentiation and activation of hematopoietic cells. Mouse G-CSF cDNA encodes a 208 amino acid (aa) precursor protein with a 30 aa signal sequence that is proteolytically cleaved to form a 178 aa O-glycosylated mature protein containing two intrachain disulfide bridges. In humans, two distinct cDNA clones, encoding a 204 aa form and a minor alternatively spliced 207 aa form of G-CSF precursors, have been isolated. Mouse and human G-CSF are 76% identical at the aa sequence level and the two proteins show species cross-reactivity. G-CSF is produced primarily by monocytes and macrophages upon activation by endotoxin, TNF-alpha or IL-1. Other cell types, including fibroblasts, endothelial cells, astrocytes and bone marrow stroma cells, can also secrete G-CSF after activation. In addition, various tumor cells express G-CSF constitutively.

Mouse G-CSF receptor (G-CSF R) is a 120 kDa, type I transmembrane glycoprotein that belongs to the hematopoietin receptor superfamily. The mature protein is 812 amino acids in length and contains a 601 aa extracellular region, a 24 aa transmembrane segment, and a 187 aa cytoplasmic domain. The extracellular region contains multiple modules, including an N-terminal Ig-like domain, a cytokine receptor homology domain, and three fibronectin type III domains. Based on crystallographic study, G-CSF receptor forms a complex with the ligand in a 2:2 ratio. Mouse and human G-CSF receptor share 63% aa sequence identity. Cells known to express G-CSF R include monocytes and neutrophils, megakaryocytes and platelets, CD34+ CD33+ and CD34+ CD38+ hematopoietic progenitors, trophoblasts, endothelial cells and various tumor cell types.

G-CSF is an important regulator for granulopoiesis in vivo. It has been demonstrated that G-CSF can support the growth of multi-lineage hematopoietic progenitor cells without influencing their commitment to the myeloid lineage and mobilize hematopoietic progenitor cells from the bone marrow into the bloodstream. On mature neutrophils, G-CSF may regulate neutrophil survival by controlling their rate of apoptosis. G-CSF has also been shown to enhance the functional capacity of mature neutrophils. As a consequence of its effects on hematopoietic progenitor cells, G-CSF has been shown to enhance monocytopoiesis in the presence M-CSF. Within the peripheral blood stem cell population mobilized with G-CSF, selective increases in the number of T helper 2-inducing dendritic cells are found.

Item	Value
Long Name	Granulocyte Colony Stimulating Factor
Entrez Gene IDs	1440 (Human); 12985 (Mouse)
Alternate Names	C17orf33, CSF3, CSF3OS, Filgrastim, G-CSF, GCSF, GCSFlenograstim, Lenograstim, MGC45931, Pluripoietin, chromosome 17 open reading frame 33, colony stimulating factor 3 (granulocyte), granulocyte colony-stimulating factor

Citations for Mouse G-CSF DuoSet ELISA (25)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse G-CSF DuoSet ELISA include:

Species: Mouse
Sample Types: Tissue Homogenates

M Kokoszynsk et al. (2021), Storage conditions of high-fat diets affect pulmonary inflammation Physiological Reports, 9(22):e15116.
PMID: 34822216
Species: Mouse
Sample Types: Cell Culture Supernates

AL Aldrich et al. (2020), TLR2 and caspase-1 signaling are critical for bacterial containment but not clearance during craniotomy-associated biofilm infection J Neuroinflammation, 17(1):114.
PMID: 32290861
Species: Mouse
Sample Types: Cell Culture Supernates

JH Song et al. (2020), Inhibitory effect of ginsenoside Rg3 on cancer stemness and mesenchymal transition in breast cancer via regulation of myeloid-derived suppressor cells PLoS ONE, 15(10):e0240533.
PMID: 33091036
Species: Mouse
Sample Types: Plasma

NE Powers et al. (2019), PASylation of IL-1 Receptor antagonist (IL-1Ra) retains IL-1 blockade and extends its duration in mouse urate crystal-induced peritonitis J. Biol. Chem., 0(0).
PMID: 31819009
Species: Mouse
Sample Types: Serum

S Lorscheid et al. (2019), Keratinocyte-derived I&kappaB? drives psoriasis and associated systemic inflammation JCI Insight, 4(22).
PMID: 31622280
Species: Mouse
Sample Types: Cell Culture Supernates

BE Hsu et al. (2019), Immature Low-Density Neutrophils Exhibit Metabolic Flexibility that Facilitates Breast Cancer Liver Metastasis Cell Rep, 27(13):3902-3915.e6.
PMID: 31242422
Species: Mouse
Sample Types: Serum

MA Meyer et al. (2018), Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance Nat Commun, 9(1):1250.
PMID: 29593283
Species: Mouse
Sample Types: Cell Culture Supernates

I Mitroulis et al. (2018), Modulation of Myelopoiesis Progenitors Is an Integral Component of Trained Immunity Cell, 172(1):147-161.e12.
PMID: 29328910
Species: Mouse
Sample Types: Plasma

I Mitroulis et al. (2017), Secreted protein Del-1 regulates myelopoiesis in the hematopoietic stem cell niche J. Clin. Invest., 0(0).
PMID: 28846069
Species: Mouse
Sample Types: BALF

MS Gresnigt et al. (2017), The Absence of NOD1 Enhances Killing of Aspergillus fumigatus Through Modulation of Dectin-1 Expression Front Immunol, 8(0):1777.
PMID: 29326692
Species: Mouse
Sample Types: Serum

(2016), Epidermal RAF prevents allergic skin disease Elife, 5(0).
PMID: 27431613
Species: Mouse
Sample Types: Plasma

(2016), The time-of-day of myocardial infarction onset affects healing through oscillations in cardiac neutrophil recruitment EMBO Mol Med, 0(0).
PMID: 27226028
Species: Mouse
Sample Types: Serum

(2016), Extracellular Superoxide Dismutase Enhances the Recruitment of Immature Neutrophils to the Liver Infect Immun, 0(0).
PMID: 27600509
Species: Mouse
Sample Types: Tissue Homogenates

A Savers et al. (2016), Infection-Mediated Priming of Phagocytes Protects against Lethal Secondary Aspergillus fumigatus Challenge PLoS ONE, 11(4):e0153829.
PMID: 27078879
Species: Mouse
Sample Types: Tissue Homogenates

(2016), Reducing hypoxia and inflammation during invasive pulmonary aspergillosis by targeting the Interleukin-1 receptor Sci Rep, 6(0):26490.
PMID: 27215684
Species: Mouse
Sample Types: Tissue Homogenates

Zhao Y et al. (2015), Thrombospondin-1 restrains neutrophil granule serine protease function and regulates the innate immune response during Klebsiella pneumoniae infection. Mucosal Immunol, 8(4):896-905.
PMID: 25492474
Species: Mouse
Sample Types: Cell Culture Supernates

Cao Y et al. (2014), BMP4 inhibits breast cancer metastasis by blocking myeloid-derived suppressor cell activity. Cancer Res, 74(18):5091-102.
PMID: 25224959
Species: Mouse
Sample Types: Tissue Homogenates

De Filippo K et al. (2014), A new protective role for S100A9 in regulation of neutrophil recruitment during invasive pneumococcal pneumonia. FASEB J, 28(8):3600-8.
PMID: 24776746
Species: Mouse
Sample Types: Plasma

Weber S et al. (2013), Regulation of adult hematopoiesis by the a disintegrin and metalloproteinase 10 (ADAM10). Biochem Biophys Res Commun, 442(3):234-41.
PMID: 24239882
Species: Mouse
Sample Types: Plasma

Bosmann M et al. (2013), The interaction between C5a and both C5aR and C5L2 receptors is required for production of G-CSF during acute inflammation. Eur J Immunol, 43(7):1907-13.
PMID: 23575697
Species: Mouse
Sample Types: Plasma

von Vietinghoff S et al. (2010), Defective regulation of CXCR2 facilitates neutrophil release from bone marrow causing spontaneous inflammation in severely NF-kappa B-deficient mice. J. Immunol., 185(1):670-8.
PMID: 20519647
Species: Mouse
Sample Types: Cell Culture Supernates

von Vietinghoff S et al. (2009), IL-17A controls IL-17F production and maintains blood neutrophil counts in mice. J. Immunol., 183(2):865-73.
PMID: 19542376
Species: Mouse
Sample Types: Serum

Smith E et al. (2007), IL-23 is required for neutrophil homeostasis in normal and neutrophilic mice. J. Immunol., 179(12):8274-9.
PMID: 18056371
Species: Mouse
Sample Types: Peritoneal Lavage Fluid

Witowski J et al. (2007), Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum. Kidney Int., 71(6):514-25.
PMID: 17228364
Species: Mouse
Sample Types: BALF

Allen GB et al. (2006), Choosing the frequency of deep inflation in mice: balancing recruitment against ventilator-induced lung injury. Am. J. Physiol. Lung Cell Mol. Physiol., 291(4):L710-7.
PMID: 16698851

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Customer Reviews for Mouse G-CSF DuoSet ELISA (3)

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Mouse G-CSF DuoSet ELISA

Name: Anonymous

Sample Tested: BMDC

Verified Customer | Posted 03/28/2018
Mouse G-CSF DuoSet ELISA

Name: Anonymous

Sample Tested: mouse serum

Verified Customer | Posted 08/04/2016
Mouse G-CSF DuoSet ELISA, 15 Plate

Name: Jacqueline Kimmey

Sample Tested: murine blood,murine lung homogenate supernatant,murine serum (blood)

Verified Customer | Posted 04/05/2016

homogenate sup was filtered through 0.22um filter before use and then diluted 1:3 for analysis.

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Product Documents for Mouse G-CSF DuoSet ELISA

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Mouse G-CSF DuoSet ELISA

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Summary for Mouse G-CSF DuoSet ELISA

Product Specifications for Mouse G-CSF DuoSet ELISA

Kit Contents for Mouse G-CSF DuoSet ELISA

Other Reagents Required

Scientific Data Examples for Mouse G-CSF DuoSet ELISA

Preparation and Storage

Background: G-CSF

Citations for Mouse G-CSF DuoSet ELISA (25)

Customer Reviews for Mouse G-CSF DuoSet ELISA (3)

Customer Images

Mouse G-CSF DuoSet ELISA

Mouse G-CSF DuoSet ELISA

Mouse G-CSF DuoSet ELISA, 15 Plate

Product Documents for Mouse G-CSF DuoSet ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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