Mouse RBP4 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MRBP40
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (25 µL)
Sensitivity
0.043 ng/mL
Assay Range
0.625-20 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Mouse RBP4 Quantikine ELISA Kit
The Quantikine Mouse RBP4 immunoassay is a 4.5 hour solid-phase ELISA designed to measure RBP4 in cell culture supernates, cell lysates, mouse serum, plasma, and urine. It contains NS0-expressed recombinant mouse RBP4 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant mouse RBP4. Results obtained using natural mouse RBP4 showed dose response curves that were parallel to the standard curve obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse RBP4.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse RBP4
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.896 | 2.23 | 9.44 | 0.902 | 2.22 | 8.52 |
| Standard Deviation | 0.036 | 0.12 | 0.51 | 0.089 | 0.15 | 0.64 |
| CV% | 4.0 | 5.4 | 5.4 | 9.9 | 6.8 | 7.5 |
Recovery for Mouse RBP4 Quantikine ELISA Kit
The recovery of mouse RBP4 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 98 | 90-112 |
| Urine (n=4) | 91 | 80-107 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse RBP4 in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse RBP4 Quantikine ELISA Kit
Mouse RBP4/Retinol-Binding Protein 4 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: RBP4/Retinol-Binding Protein 4
Retinol-binding protein 4 (RBP4) is a Lipocalin superfamily molecule that transports vitamin A (retinol) and retinaldehyde in the serum. Retinaldehyde is the critical chromophore in the rhodopsin photoreceptor, while both it and retinoic acid regulate a multitude of cellular differentiation and proliferation effects through the intracellular receptors RAR and RXR. RBP4 is secreted primarily by hepatocytes and adipocytes into the blood where the RBP4-retinol complex interacts with transthyretin (TTR). Formation of the complex with TTR increases the serum half-life of RBP4 by preventing RBP4 filtration through the kidney. The C-terminally processed forms of RBP4, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. RBP4 promotes hyperglycemia, and its upregulation in visceral and liver adipocytes leads to elevated serum levels.
Alternate Names
RetinolBinding Protein 4
Gene Symbol
RBP4
Additional RBP4/Retinol-Binding Protein 4 Products
Product Documents for Mouse RBP4 Quantikine ELISA Kit
Product Specific Notices for Mouse RBP4 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.