Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit Best Seller

Name: Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit
Brand: R&D Systems
SKU: MOST00
Rating: 5 (1 reviews)

Catalog # MOST00 | R&D Systems, Inc. a Bio-Techne Brand

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Citations (43)

Product Datasheet / COA / SDS

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL)
Sensitivity:	8.5 pg/mL
Assay Range:	39 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit Summary:	The Quantikine Mouse OPN Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse OPN in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse OPN and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse OPN showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse OPN.

View Full Technical Details for Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit

Product Specifications for Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Mouse, Rat
Specificity	Natural and recombinant mouse and rat OPN. This assay cross-reacts 8.2% with recombinant Human Osteopontin.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	62	67	59
Mean (pg/mL)	96	236	1085	116	279	1076
Standard Deviation	7.3	13.8	43.4	9.8	22.5	90
CV%	7.6	5.8	4	8.4	8.1	8.4

Recovery

The recovery of mouse OPN spiked to three levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Mouse Cell Culture Supernates (n=7)	98	87-106
Rat Cell Culture Supernates (n=7)	93	82-107

Linearity

To assess the linearity of the assay, samples containing mouse or rat OPN were diluted with Calibrator Diluent and then assayed.

Scientific Data Examples for Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit

	Mouse/Rat Osteopontin/OPN ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Osteopontin/OPN

Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell ad¬hesion, and migration. Its expression is upregulat¬ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.

Item	Value
Long Name	Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs	6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names	BNSP, Bone sialoprotein 1, Eta-1, MGC110940, Nephropontin, OPN, Osteopontin, SPP-1, SPP1/CALPHA1 fusion, Spp1, Urinary stone protein, secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1), secreted phosphoprotein-1 (osteopontin, bone sialoprotein), uropontin

Citations for Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit (43)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit include:

Species: Mouse
Sample Types: Serum

KG English et al. (2022-09-26), Next-Generation SINE Compound KPT-8602 Ameliorates Dystrophic Pathology in Zebrafish and Mouse Models of DMD Biomedicines, 2022-010(10).
PMID: 36289662
Species: Rat
Sample Types: Urine

MJ Coyne et al. (2022-05-27), Evaluation of renal injury and function biomarkers, including symmetric dimethylarginine (SDMA), in the rat passive Heymann nephritis (PHN) model PLoS ONE, 2022-017(5):e0269085.
PMID: 35622875
Species: Mouse
Sample Types: Serum

O Mucha et al. (2021-12-31), Dysregulated Autophagy and Mitophagy in a Mouse Model of Duchenne Muscular Dystrophy Remain Unchanged Following Heme Oxygenase-1 Knockout International Journal of Molecular Sciences, 2021-123(1).
PMID: 35008897
Species: Human, Mouse
Sample Types: Cell Culture Supernates, Serum

X Jiang et al. (2021-02-27), Lipid-injured hepatocytes release sOPN to improve macrophage migration via CD44 engagement and pFak-NF&kappaB signaling Cytokine, 2021-0142(0):155474.
PMID: 33647584
Species: Mouse
Sample Types: Serum

TA Pereira et al. (2021-01-23), Praziquantel pharmacotherapy reduces systemic osteopontin levels and liver collagen content in murine schistosomiasis mansoni International journal for parasitology, 2021-00(0).
PMID: 33493521
Species: Mouse
Sample Types: Plasma

K Ritter et al. (2021-01-18), Early Reciprocal Effects in a Murine Model of Traumatic Brain Injury and Femoral Fracture Mediators of Inflammation, 2021-02021(0):8835730.
PMID: 33531878
Species: Rat
Sample Types: Urine

K Kohl et al. (2020-08-07), Evaluation of urinary biomarkers for early detection of acute kidney injury in a rat nephropathy model J Pharmacol Toxicol Methods, 2020-00(0):106901.
PMID: 32771565
Species: Rat
Sample Types: Plasma

A Frasca et al. (2020-08-05), Glycation and Serum Albumin Infiltration Contribute to the Structural Degeneration of Bioprosthetic Heart Valves JACC Basic Transl Sci, 2020-05(8):755-766.
PMID: 32875167
Species: Mouse
Sample Types: Plasma

J Wilflingse et al. (2020-07-07), Enhancer and super-enhancer dynamics in repair after ischemic acute kidney injury Nat Commun, 2020-011(1):3383.
PMID: 32636391
Species: Mouse
Sample Types: Cell Culture Supernates

B Gómez-Sant et al. (2020-07-07), Liver osteopontin is required to prevent the progression of age-related nonalcoholic fatty liver disease Aging Cell, 2020-00(0):e13183.
PMID: 32638492
Species: Mouse
Sample Types: Cell Culture Supernates

PG Hitscheric et al. (2019-07-01), The effects of macrophages on cardiomyocyte calcium-handling function using in vitro culture models Physiol Rep, 2019-07(13):e14137.
PMID: 31301118
Species: Human
Sample Types: Whole Cells

B Giri et al. (2019-05-20), Pre-clinical evaluation of Minnelide as a therapy for acute myeloid leukemia J Transl Med, 2019-017(1):163.
PMID: 31109340
Species: Mouse
Sample Types: Cell Culture Supernates

E Giraud et al. (2019-02-08), Osteopontin in the host response to Leishmania amazonensis BMC Microbiol., 2019-019(1):32.
PMID: 30736736
Species: Mouse
Sample Types: Serum

J Wang et al. (2019-01-09), Osteopontin potentiates PM-induced IL-1? and IL-1? production via the ERK/JNK signaling pathway Ecotoxicol. Environ. Saf., 2019-0171(0):467-474.
PMID: 30639873
Species: Mouse
Sample Types: Serum

JD Klement et al. (2018-11-05), An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion J. Clin. Invest., 2018-10(0).
PMID: 30395540
Species: Mouse
Sample Types: Cell Culture Supernates

MN Lee et al. (2018-11-05), Elevated extracellular calcium ions promote proliferation and migration of mesenchymal stem cells via increasing osteopontin expression Exp. Mol. Med., 2018-150(11):142.
PMID: 30393382
Species: Mouse
Sample Types: Cell Culture Supernates

E De Giglio et al. (2018-10-11), Multi-compartment scaffold fabricated via 3D-printing as in vitro co-culture osteogenic model Sci Rep, 2018-18(1):15130.
PMID: 30310164
Species: Mouse
Sample Types: Serum

M Nuñez-Garc et al. (2018-10-02), Atorvastatin provides a new lipidome improving early regeneration after partial hepatectomy in osteopontin deficient mice Sci Rep, 2018-18(1):14626.
PMID: 30279550
Species: Mouse
Sample Types: Serum

ZV Varga et al. (2018-10-01), Disruption of renal arginine metabolism promotes kidney injury in hepatorenal syndrome Hepatology, 2018-10(0).
PMID: 29631342
Species: Mouse
Sample Types: Plasma

M Mastri et al. (2018-04-25), Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors Mol. Cancer Ther., 2018-00(0).
PMID: 29695634
Species: Mouse
Sample Types: Serum

LA O'Reilly et al. (2018-03-20), Loss of NF-?B1 Causes Gastric Cancer with Aberrant Inflammation and Expression of Immune Checkpoint Regulators in a STAT-1-Dependent Manner Immunity, 2018-048(3):570-583.e8.
PMID: 29562203
Species: Mouse
Sample Types: Plasma

F Blanco et al. (2018-03-02), In vivo inhibition of nuclear factor of activated T-cells leads to atherosclerotic plaque regression in IGF-II/LDLR-/-ApoB100/100mice Diab Vasc Dis Res, 2018-00(0):1479164118759.
PMID: 29499628
Species: Rat
Sample Types: Serum

N Wilck et al. (2018-02-22), Nitric oxide-sensitive guanylyl cyclase stimulation improves experimental heart failure with preserved ejection fraction JCI Insight, 2018-03(4).
PMID: 29467337
Species: Mouse
Sample Types: Cell Culture Supernates

K Fu et al. (2017-10-12), Involvement of the accumbal osteopontin-interacting transmembrane protein 168 in methamphetamine-induced place preference and hyperlocomotion in mice Sci Rep, 2017-17(1):13084.
PMID: 29026117
Species: Mouse
Sample Types: Plasma

A Aarup et al. (2017-07-26), Uremia does not affect neointima formation in mice Sci Rep, 2017-07(1):6496.
PMID: 28747676
Species: Mouse
Sample Types: Cell Culture Supernates

GM Pio et al. (2017-05-12), Soluble bone-derived osteopontin promotes migration and stem-like behavior of breast cancer cells PLoS ONE, 2017-012(5):e0177640.
PMID: 28498874
Species: Mouse
Sample Types: Cell Culture Supernates

Fuquan Jin (2017-01-15), Deficient TSC1/TSC2-complex suppression of SOX9-osteopontin-AKT signalling cascade constrains tumour growth in tuberous sclerosis complex Hum. Mol. Genet, 2017-00(0).
PMID: 28013293
Species: Rat
Sample Types: Cell Culture Supernates

J Grzesiak et al. (2017-01-03), Ultrastructural changes during osteogenic differentiation in mesenchymal stromal cells cultured in alginate hydrogel Cell Biosci, 2017-07(0):2.
PMID: 28066541
Species: Mouse
Sample Types: Tissue Homogenates

(2016-05-13), The P2X4 receptor is required for neuroprotection via ischemic preconditioning Sci Rep, 2016-06(0):25893.
PMID: 27173846
Species: Mouse
Sample Types: Cell Culture Supernates

(2016-05-02), Age-associated vascular inflammation promotes monocytosis during atherogenesis Aging Cell, 2016-00(0).
PMID: 27135421
Species: Rat
Sample Types: Urine

Huang H et al. (2015-08-04), High Sodium-Induced Oxidative Stress and Poor Anticrystallization Defense Aggravate Calcium Oxalate Crystal Formation in Rat Hyperoxaluric Kidneys. PLoS ONE, 2015-010(8):e0134764.
PMID: 26241473
Species: Mouse
Sample Types: Plasma

Zetterqvist A et al. (2015-03-31), Nuclear factor of activated T cells is activated in the endothelium of retinal microvessels in diabetic mice. J Diabetes Res, 2015-02015(0):428473.
PMID: 25918731
Species: Mouse
Sample Types: Serum

Zelenchuk L et al. (2014-05-19), PHEX mimetic (SPR4-peptide) corrects and improves HYP and wild type mice energy-metabolism. PLoS ONE, 2014-09(5):e97326.
PMID: 24839967
Species: Rat
Sample Types: Cell Culture Supernates

Chang S et al. (2014-02-04), Phenotypic modulation of primary vascular smooth muscle cells by short-term culture on micropatterned substrate. PLoS ONE, 2014-09(2):e88089.
PMID: 24505388
Species: Mouse
Sample Types: Tissue Homogenates

Brampton C et al. (2014-01-01), The level of hepatic ABCC6 expression determines the severity of calcification after cardiac injury. Am J Pathol, 2014-0184(1):159-70.
PMID: 24479134
Species: Mouse
Sample Types: Cell Culture Supernates

Chen J et al. (2013-07-27), Plasma membrane Pdia3 and VDR interact to elicit rapid responses to 1alpha,25(OH)(2)D(3). Cell Signal, 2013-025(12):2362-73.
PMID: 23896121
Species: Mouse
Sample Types: Serum

Shojaei F et al. (2012-03-23), Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer. J. Exp. Clin. Cancer Res., 2012-031(0):26.
PMID: 22444159
Species: Rat
Sample Types: Tissue Homogenates

Schupp N et al. (2010-12-06), Mineralocorticoid receptor-mediated DNA damage in kidneys of DOCA-salt hypertensive rats. FASEB J., 2010-125(3):968-78.
PMID: 21135038
Species: Mouse
Sample Types: Cell Culture Supernates

Chen J et al. (2010-09-15), Protein-disulfide isomerase-associated 3 (Pdia3) mediates the membrane response to 1,25-dihydroxyvitamin D3 in osteoblasts. J. Biol. Chem., 2010-0285(47):37041-50.
PMID: 20843786
Species: Mouse
Sample Types: Plasma

Kiefer FW et al. (2010-01-27), Neutralization of osteopontin inhibits obesity-induced inflammation and insulin resistance. Diabetes, 2010-059(4):935-46.
PMID: 20107108
Species: Mouse
Sample Types: Serum

Takanashi M et al. (2009-05-28), Therapeutic silencing of an endogenous gene by siRNA cream in an arthritis model mouse. Gene Ther., 2009-016(8):982-9.
PMID: 19474812
Species: Rat
Sample Types: Cell Culture Supernates

Shao J et al. (2007-08-11), Heterozygous disruption of the PTEN promotes intestinal neoplasia in APCmin/+ mouse: roles of osteopontin. Carcinogenesis, 2007-028(12):2476-83.
PMID: 17693663
Species: Mouse
Sample Types: Plasma

Briscoe CP et al. (2007-07-10), LPS-induced biomarkers in mice: a potential model for identifying insulin sensitizers. Biochem. Biophys. Res. Commun., 2007-0361(1):140-5.
PMID: 17659259

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Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit

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Sample Tested: Serum

Verified Customer | Posted 01/16/2022

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.