Mouse Pentraxin 3/TSG-14 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MPTX30
Discontinued Product
MPTX30 has been discontinued.
View all Pentraxin 3/TSG-14 products.
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL)
Sensitivity
0.02 ng/mL
Assay Range
0.23-15 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse Pentraxin 3/TSG-14 Quantikine ELISA Kit
The Quantikine Mouse Pentraxin 3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse PTX3 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse PTX3 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse PTX3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse PTX3.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse PTX3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 33 | 34 | 34 |
| Mean (ng/mL) | 0.54 | 2.09 | 6.86 | 0.63 | 2.28 | 7.18 |
| Standard Deviation | 0.01 | 0.05 | 0.20 | 0.07 | 0.16 | 0.41 |
| CV% | 1.9 | 2.4 | 2.9 | 11.1 | 7.0 | 5.7 |
Recovery for Mouse Pentraxin 3/TSG-14 Quantikine ELISA Kit
The recovery of mouse PTX3 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 107 | 98-119 |
| EDTA Plasma (n=4) | 98 | 90-110 |
| Heparin Plasma (n=4) | 95 | 81-100 |
| Serum (n=4) | 102 | 90-117 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of mouse PTX3 in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse Pentraxin 3/TSG-14 Quantikine ELISA Kit
Mouse Pentraxin 3/TSG-14 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Pentraxin 3/TSG-14
Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.
Long Name
Tumor Necrosis Factor Stimulated Gene 14
Alternate Names
PTX3, TSG-14, TSG14
Gene Symbol
PTX3
Additional Pentraxin 3/TSG-14 Products
Product Documents for Mouse Pentraxin 3/TSG-14 Quantikine ELISA Kit
Product Specific Notices for Mouse Pentraxin 3/TSG-14 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.