Mouse CD40 Quantikine ELISA Kit

Name: Mouse CD40 Quantikine ELISA Kit
Brand: R&D Systems
SKU: MCCD40

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MCCD40

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sample Types:	Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
Sensitivity:	3.7 pg/mL
Assay Range:	7.81 - 500 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Mouse CD40 Quantikine ELISA Kit Summary:	The Quantikine Mouse CD40 Immunoassay is a 4.5 hour solid phase ELISA designed to measure CD40 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant mouse CD40 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural CD40 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse CD40.

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Product Specifications for Mouse CD40 Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Mouse
Specificity	Natural and recombinant mouse CD40
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	21	67.2	159	22.7	70.9	169
Standard Deviation	1.5	3.3	5.2	1.7	2.9	7.8
CV%	7.1	4.9	3.3	7.5	4.1	4.6

Recovery

The recovery of mouse CD40 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	102	86-119
EDTA Plasma (n=4)	96	82-109
Heparin Plasma (n=4)	92	83-109
Serum (n=4)	92	81-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse CD40 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Mouse CD40 Quantikine ELISA Kit

	Mouse CD40/TNFRSF5 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD40/TNFRSF5

CD40, also known as TNFRSF5, is a transmembrane TNF superfamily receptor that is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells. Interaction of CD40 with CD40 Ligand leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40 Ligand expressing cells. This interaction promotes B cell activation and T cell-dependent humoral responses. CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy. Dysregulation of CD40/CD40 Ligand expression and interactions contributes to disease severity in AIDS, atherosclerosis, atherothrombosis, and restenosis.

Entrez Gene IDs

958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)

Alternate Names

B cell surface antigen CD40, B-cell surface antigen CD40, Bp50B cell-associated molecule, CD40, CD40 antigen, CD40 molecule, TNF receptor superfamily member 5, CD40 type II isoform, CD40L receptor, CDw40, MGC9013, TNFRSF5, TNFRSF5CD40 antigen (TNF receptor superfamily member 5), nerve growth factor receptor-related B-lymphocyte activation molecule, p50, tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5

Citations for Mouse CD40 Quantikine ELISA Kit (1)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse CD40 Quantikine ELISA Kit include:

Species: Mouse
Sample Types: Plasma

P Jiang et al. (2017), Hydrogen sulfide protects against the development of experimental cerebral malaria in a C57BL/6 mouse model Mol Med Rep, 0(0).
PMID: 28656241

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Product Documents for Mouse CD40 Quantikine ELISA Kit

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.