Skip to main content

Mouse IL-2 DuoSet ELISA

Catalog # DY402 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
DY402
DY402-05

Ancillary Products Available

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

15.6-1000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Mouse

Mouse IL-2 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Product Summary for Mouse IL-2 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Label

HRP

Scientific Data Images for Mouse IL-2 DuoSet ELISA

Mouse IL-2 ELISA Standard Curve

Mouse IL-2 ELISA Standard Curve

Kit Contents for Mouse IL-2 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Block Buffer: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Reagent Diluent: 0.1% BSA, 0.05% Tween 20 in Tris-buffered Saline (20 mM Trizma base, 150 mM NaCI) pH 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-2

IL-2 (Interleukin 2) is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex. Gamma c is also a signaling subunit in the receptors for IL-4, -7, -9, -15, and -21.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Gene Symbol

IL2

Product Documents for Mouse IL-2 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IL-2 DuoSet ELISA

For research use only

Citations for Mouse IL-2 DuoSet ELISA (82)

Showing  1 - 10 of 82 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Mouse IL-2 DuoSet ELISA include:

There are no citations that match your criteria.

Showing  1 - 10 of 82 publications Showing All

Customer Reviews for Mouse IL-2 DuoSet ELISA (2)

4 out of 5
2 customer ratings
5 stars
0%
4 stars
100%
3 stars
0%
2 stars
0%
1 star
0%

Customer Images


Showing  1 - 2 of 2 reviews Showing All
Filter By:
  • Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 11/02/2017
  • Mouse IL-2 DuoSet ELISA, 15 Plate
    Name: Anonymous
    Sample Tested: Lymphocytes (ex vivo)
    Verified Customer | Posted 10/26/2015
    Specificity: Specific
    Sensitivity: Sensitive
    Buffer: 1% BSA in PBS, pH 7.2-7.4, filtered
    Dilution: 1/180
    Mouse IL-2 DuoSet ELISA DY402

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All
Loading...
Setting the standard in quality research reagents