Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MFK00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL)
Sensitivity
5 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum)
Product Summary for Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit
The Quantikine Mouse/Rat Flt-3 Ligand Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat Flt-3 Ligand levels in cell culture supernates and serum. It contains NS0-expressed recombinant mouse Flt-3 Ligand and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse or rat Flt-3 Ligand showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse/rat Flt-3 Ligand.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Natural and recombinant mouse Flt-3 Ligand and natural rat Flt-3 Ligand
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 79.5 | 237 | 775 | 82.4 | 242 | 768 |
| Standard Deviation | 3.9 | 10.1 | 28.6 | 6.2 | 13.6 | 39.4 |
| CV% | 4.9 | 4.3 | 3.7 | 7.5 | 5.6 | 5.1 |
Recovery for Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit
The recovery of mouse Flt-3 Ligand spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Supernates (n=5) | 99 | 88-107 |
| Mouse Serum (n=5) | 95 | 82-106 |
| Rat Cell Culture Supernates (n=5) | 108 | 99-119 |
| Rat Serum (n=5) | 95 | 86-114 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse Flt-3 Ligand in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit
Mouse/Rat Flt-3 Ligand ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Flt-3 Ligand/FLT3L
Flt-3 Ligand, also known as FLT3L, is a cytokine that promotes the differentiation or expansion of multiple hematopoietic cell lineages including monocytes, immature dendritic cells, and early B cell lineage cells. It synergizes with other cytokines to promote the myeloid differentiation and mobilization of hematopoietic stem cells, to induce NK cell development, and to induce terminal B cell maturation. Flt-3 Ligand/FLT3L is expressed as a dimer by T cells and bone marrow and thymic fibroblasts. Both transmembrane and soluble forms of Flt-3 Ligand/FLT3L signal through the tyrosine kinase receptor Flt-3/Flk-2.
Long Name
fms-like Tyrosine Kinase 3 Ligand
Alternate Names
FLG3L, Flt3L, FLT3LG
Gene Symbol
FLT3LG
Additional Flt-3 Ligand/FLT3L Products
Product Documents for Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.