Mouse CXCL13/BLC/BCA-1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MCX130
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL)
Sensitivity
2.84 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse CXCL13/BLC/BCA-1 Quantikine ELISA Kit
The Quantikine Mouse BLC/BCA-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse BLC/BCA-1 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse BLC/BCA-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse BLC/BCA-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse BLC/BCA-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse BLC/BCA-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 51 | 109 | 408 | 52 | 104 | 366 |
| Standard Deviation | 3.1 | 3.8 | 15.4 | 5.1 | 7.1 | 27.1 |
| CV% | 6.1 | 3.5 | 3.8 | 9.8 | 6.8 | 7.4 |
Recovery for Mouse CXCL13/BLC/BCA-1 Quantikine ELISA Kit
The recovery of mouse BLC/BCA-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 93 | 83-109 |
| EDTA Plasma (n=7) | 98 | 80-115 |
| Heparin Plasma (n=4) | 96 | 80-110 |
| Serum (n=7) | 104 | 85-119 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of mouse BLC/BCA-1 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse CXCL13/BLC/BCA-1 Quantikine ELISA Kit
Mouse CXCL13/BLC/BCA-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL13/BLC/BCA-1
CXCL13/BLC/BCA-1 is a constitutively expressed chemokine that plays an important role in B and T cell homing. It is expressed by salivary gland epithelium, dendritic cells, osteoclasts, and peritoneal macrophages. It can form homodimers or heterodimers with FGF basic, and it signals through CXCR3 or CXCR5. CXCL13 induces the migration of naive B cells and a subset of memory T cells to lymphoid tissue. It also promotes B1 cell migration into the omentum and peritoneum. In the fetus, CXCL13 attracts CD4+ CD3- IL-7 R alpha+ hematopoietic cells to sites of future Peyer's patch development.
Alternate Names
ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13
Gene Symbol
CXCL13
Additional CXCL13/BLC/BCA-1 Products
Product Documents for Mouse CXCL13/BLC/BCA-1 Quantikine ELISA Kit
Product Specific Notices for Mouse CXCL13/BLC/BCA-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.