Mouse Pentraxin 2/SAP Quantikine ELISA Kit

Name: Mouse Pentraxin 2/SAP Quantikine ELISA Kit
Brand: R&D Systems
SKU: MPTX20

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MPTX20

Citations (4)

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Key Product Details

Assay Length:	4.5
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sample Types:	Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Sensitivity:	0.368 ng/mL
Assay Range:	1.56 - 100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Mouse Pentraxin 2/SAP Quantikine ELISA Kit Summary:	The Quantikine Mouse PTX2/SAP Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse PTX2/SAP in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse PTX2/SAP and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse PTX2/SAP showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse PTX2/SAP.

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Product Specifications for Mouse Pentraxin 2/SAP Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Mouse
Specificity	Natural and recombinant mouse PTX2/SAP
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	4.11	16	33.6	3.95	15.7	34.5
Standard Deviation	0.22	0.57	1.33	0.33	0.76	1.59
CV%	5.4	3.6	4	8.4	4.8	4.6

Recovery

The recovery of PTX2/SAP spiked into cell culture samples was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Samples (n=6)	97	81-112

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse PTX2/SAP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Mouse Pentraxin 2/SAP Quantikine ELISA Kit

	Mouse Pentraxin 2/SAP ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Pentraxin 2/SAP

Pentraxin or Pentraxin 2 (PTX2), also known as Serum Amyloid P component (SAP), is a secreted serum glycoprotein belonging to the pentraxin superfamily. Pentraxins have a characteristic pentagonal discoid arrangement of five non-covalently bound subunits and bind a variety of molecules in a calcium- dependent lectin-like manner through a pattern-recognition-binding site. PTX2 is a major acute-phase protein and may function as an opsonin for a variety of ligands. PTX2 also associates ubiquitously with all amyloid deposits implicated in diseases including Alzheimer's and prion diseases, type 2 diabetes and various systemic amyloidoses.

Item	Value
Long Name	Serum Amyloid P Component
Entrez Gene IDs	325 (Human); 20219 (Mouse); 29339 (Rat)
Alternate Names	9.5S alpha-1-glycoprotein, APCS, MGC88159, PTX2, PTX2serum amyloid P-component, Pentraxin 2, SAP, SAPpentaxin-related, Serum Amyloid P component, amyloid P component, serum

Citations for Mouse Pentraxin 2/SAP Quantikine ELISA Kit (4)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse Pentraxin 2/SAP Quantikine ELISA Kit include:

Species: Mouse
Sample Types: Serum

JA Poole et al. (2022), Increased susceptibility to organic dust exposure-induced inflammatory lung disease with enhanced rheumatoid arthritis-associated autoantigen expression in HLA-DR4 transgenic mice Respiratory Research, 23(1):160.
PMID: 35717175
Species: Mouse
Sample Types: Serum

TR Mikuls et al. (2021), The impact of airborne endotoxin exposure on rheumatoid arthritis-related joint damage, autoantigen expression, autoimmunity, and lung disease International immunopharmacology, 100(0):108069.
PMID: 34461491
Species: Mouse
Sample Types: Plasma

JR Burgos et al. (2019), LPS immune challenge reduces arcuate nucleus TSHR and CART mRNA and elevates plasma CART peptides BMC Neurosci, 20(1):59.
PMID: 31829131
Species: Mouse
Sample Types: Plasma

H Kida et al. (2017), Pathogenesis of Lethal Aspiration Pneumonia in Mecp2-null Mouse Model for Rett Syndrome Sci Rep, 7(1):12032.
PMID: 28931890

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Product Documents for Mouse Pentraxin 2/SAP Quantikine ELISA Kit

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.