Mouse CXCL16 DuoSet ELISA

Name: Mouse CXCL16 DuoSet ELISA
Brand: R&D Systems
SKU: DY503

Catalog # DY503 | R&D Systems, Inc. a Bio-Techne Brand

Citations (11)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	15.6 - 1,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Mouse CXCL16 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Technical Data
Citations (11) / Reviews

Summary for Mouse CXCL16 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse CXCL16. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Mouse CXCL16 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Mouse
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Mouse CXCL16 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Mouse CXCL16 DuoSet ELISA

	Mouse CXCL16 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL16

CXC chemokine ligand 16 (CXCL16) is a type I membrane protein that has a non-ELR CXC chemokine domain in its extracellular region. Fractalkine (CX3CL1) and CXCL16 constitute the only two transmembrane chemokines. Mouse and human CXCL16 share 70% amino acid sequence similarity within their chemokine domains and 49% overall amino acid sequence identity.

Item	Value
Entrez Gene IDs	58191 (Human); 66102 (Mouse)
Alternate Names	CXC chemokine ligand 16, CXCL16, CXCLG16, SCYB16, SR-PSOXTransmembrane chemokine CXCL16, SRPSOXC-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, chemokine (C-X-C motif) ligand 16

Citations for Mouse CXCL16 DuoSet ELISA (11)

Showing 1 - 10 of 11 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse CXCL16 DuoSet ELISA include:

Species: Mouse
Sample Types: Tissue Homogenates

TM Savage et al. (2023-03-08), Chemokines expressed by engineered bacteria recruit and orchestrate antitumor immunity Science Advances, 2023-09(10):eadc9436.
PMID: 36888717
Species: Mouse
Sample Types: Tissue Homogenates

A Vorontsova et al. (2023-01-16), Chemotherapy-induced tumor immunogenicity is mediated in part by megakaryocyte-erythroid progenitors Oncogene, 2023-00(0).
PMID: 36646904
Species: Mouse
Sample Types: BALF

V Besnard et al. (2018-03-08), Identification of periplakin as a major regulator of lung injury and repair in mice JCI Insight, 2018-03(5).
PMID: 29515024
Species: Mouse
Sample Types: Serum

HD Brightbill et al. (2018-01-12), NF-?B inducing kinase is a therapeutic target for systemic lupus erythematosus Nat Commun, 2018-09(1):179.
PMID: 29330524
Species: Mouse
Sample Types: Serum

(2016-10-31), Neutrophils license iNKT cells to regulate self-reactive mouse B cell responses Nat. Immunol., 2016-10(0).
PMID: 27798616
Species: Mouse
Sample Types: Cell Culture Supernates

Chou C et al. (2015-03-30), Lysophosphatidic acid alters the expression profiles of angiogenic factors, cytokines, and chemokines in mouse liver sinusoidal endothelial cells. PLoS ONE, 2015-010(3):e0122060.
PMID: 25822713
Species: Mouse
Sample Types: Urine

Wu T et al. (2007-11-15), Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis. J. Immunol., 2007-1179(10):7166-75.
PMID: 17982109
Species: Mouse
Sample Types: Tissue Homogenates

Koedel U et al. (2007-07-01), Acute brain injury triggers MyD88-dependent, TLR2/4-independent inflammatory responses. Am. J. Pathol., 2007-0171(1):200-13.
PMID: 17591966
Species: Mouse
Sample Types: Urine

Wu T et al. (2007-03-01), Excreted urinary mediators in an animal model of experimental immune nephritis with potential pathogenic significance. Arthritis Rheum., 2007-056(3):949-59.
PMID: 17328072
Species: Mouse
Sample Types: Tissue Homogenates

Klein M et al. (2006-01-18), Protein expression pattern in experimental pneumococcal meningitis. Microbes Infect., 2006-08(4):974-83.
PMID: 16488173
Species: Mouse
Sample Types: Cell Culture Supernates

Biswas SK et al. (2005-11-03), A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation). Blood, 2005-1107(5):2112-22.
PMID: 16269622

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