Mouse Chemerin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MCHM00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
3.47 pg/mL
Assay Range
46.9-3000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse Chemerin Quantikine ELISA Kit
The Quantikine Mouse Chemerin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Chemerin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature Chemerin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Chemerin. Results obtained using natural mouse Chemerin showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Chemerin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse Chemerin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 153 | 405 | 1124 | 168 | 385 | 1065 |
| Standard Deviation | 13.0 | 16.2 | 99.0 | 14.2 | 25.7 | 64.1 |
| CV% | 8.5 | 4.0 | 8.8 | 8.4 | 6.7 | 6.0 |
Recovery for Mouse Chemerin Quantikine ELISA Kit
The recovery of mouse Chemerin spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 102 | 89-117 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Chemerin in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse Chemerin Quantikine ELISA Kit
Mouse Chemerin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Chemerin
Chemerin, also known as Tazarotene-induced Gene 2, (Tig-2), is a member of the Cystatin superfamily. Chemerin is synthesized as a 163 aa precursor that contains a hydrophobic 20 aa N-terminal sequence, an intervening 137 aa Cystatin fold-containing domain, and a six aa C-terminal prosegment. It is activated by proteases of the coagulation, fibrinolytic, and inflammatory cascades. Chemerin is a chemoattractant for cells bearing the Chem R23 receptor, including plasmacytoid dendritic cells and tissue macrophages.
Long Name
Retinoic Acid Receptor Responder Protein 2
Alternate Names
RARRES2, TIG-2
Gene Symbol
RARRES2
Additional Chemerin Products
Product Documents for Mouse Chemerin Quantikine ELISA Kit
Product Specific Notices for Mouse Chemerin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.