Mouse sP-Selectin/CD62P Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MPS00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL)
Sensitivity
0.018 ng/mL
Assay Range
0.31-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Product Summary for Mouse sP-Selectin/CD62P Quantikine ELISA Kit
The Quantikine Mouse soluble P-Selectin (sP-Selectin) Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sP-Selectin levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant mouse sP-Selectin and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse sP-Selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sP-Selectin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse sP-Selectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 32 | 36 | 30 |
| Mean (ng/mL) | 1.27 | 2.92 | 12.9 | 1.27 | 2.90 | 12.6 |
| Standard Deviation | 0.10 | 0.23 | 1.13 | 0.08 | 0.24 | 1.13 |
| CV% | 7.9 | 7.9 | 8.8 | 6.3 | 8.3 | 9.0 |
Recovery for Mouse sP-Selectin/CD62P Quantikine ELISA Kit
The recovery of mouse sP-Selectin spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 100 | 89-110 |
| EDTA Plasma (n=6) | 94 | 80-112 |
| Serum (n=10) | 98 | 81-115 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse sP-Selectin in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse sP-Selectin/CD62P Quantikine ELISA Kit
Mouse P-Selectin/CD62P ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: P-Selectin/CD62P
P-Selectin, also known as CD62P, GMP-140, PADGEM, and LECAM-3, is a transmembrane adhesion protein that is upregulated on activated platelets and vascular endothelial cells. It binds to hematopoietic cell PSGL-1/CD162 with highly clustered O-linked sialyl Lewis-x (sLex ) tetrasaccharides and sulfated tyrosine, as well as to heparin sulfate, Versican, and CD42b/GPIb alpha. P-Selectin mediates the initial interaction of circulating leukocytes with activated endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. It is therefore critical for leukocyte extravasation at sites of inflammation and leukocyte involvement in thrombus formation at sites of vascular injury.
Alternate Names
CD62P, GMP140, GRMP, PADGEM, PSEL, SELP
Gene Symbol
SELP
Additional P-Selectin/CD62P Products
Product Documents for Mouse sP-Selectin/CD62P Quantikine ELISA Kit
Product Specific Notices for Mouse sP-Selectin/CD62P Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.