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Mouse IL-6 DuoSet ELISA

R&D Systems, Inc. a Bio-Techne Brand

Mouse IL-6 ELISA Standard Curve
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DY406-05
DY406

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Key Product Details

Assay Type: Solid Phase Sandwich ELISA
Assay Range: 15.6 - 1,000 pg/mL
Sample Types: Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Mouse IL-6 DuoSet ELISA Features:
  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Citations for Mouse IL-6 DuoSet ELISA (515)

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Customer Reviews for Mouse IL-6 DuoSet ELISA (34)

4.8 out of 5
34 customer ratings
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  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: A549 human lung carcinoma cell line
    Verified Customer | Posted 12/05/2022
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 11/07/2022
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: BAL fluid
    Verified Customer | Posted 07/21/2022
    Very stable and simple
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Colon tissue
    Verified Customer | Posted 05/26/2022
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 03/11/2022
    Used this kit for quantifying mouse IL-6 in serum samples, could see the detection but not strong in our samples. Standard curve looks good.
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Rob Knight
    Sample Tested: Cell Culture Media,RAW 264.7 mouse monocyte/macrophage cell line
    Verified Customer | Posted 12/09/2021
    RAW Cells cultured with 10ng/mL LPS for 24hrs
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Martin Torres
    Sample Tested: Adult small intestine
    Verified Customer | Posted 09/24/2021
    Mouse IL-6 was evaluated in intestinal tissues from C57BL/6J mice treated with Dextran sodium sulfate (DSS) as chemical pro-inflammatory drug aiming to simulate chronic colitis (DSS: 2.5%, 7 days treatment). Results were consistent to what was expected from this assay, and were in agreement with previous reports for this mouse strain. Treatments correspond to Moringa oleifera powder administered to mice (20 and 50% of total caloric content).
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Ricardo Malvicini
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 09/10/2021
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 04/03/2021
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Wuyi Wang
    Sample Tested: NIH-3T3 mouse embryonic fibroblast cell line
    Verified Customer | Posted 10/28/2020
  • Mouse IL-6 DuoSet ELISA
    Name: Cristina Caldari
    Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line
    Verified Customer | Posted 08/28/2020
    Excellent kit. Easy to use, clear instructions, performs perfectly and consistently.
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: CHO Chinese hamster ovary cell line
    Verified Customer | Posted 11/21/2019
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant,RAW 264.7 mouse monocyte/macrophage cell line
    Verified Customer | Posted 11/10/2019
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: C2C12 Cell Culture Supernatants
    Verified Customer | Posted 07/10/2019
    We used this DuoSet for looking at IL-6 within the supernatant of C2C12 myotubes stimulated with various ligands. We were able to run the samples neat and received great results.
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: PT18 mouse mast/basophil cell line
    Verified Customer | Posted 03/13/2019
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 12/17/2018
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Ivan Luzardo
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 12/11/2018
  • Mouse IL-6 DuoSet ELISA
    Name: Ivan Luzardo
    Sample Tested: 3T3-L1 mouse embryonic fibroblast adipose-like cell line
    Verified Customer | Posted 12/11/2018
  • Mouse IL-6 DuoSet ELISA
    Name: Cristina Perez-Ternero
    Sample Tested: Plasma
    Verified Customer | Posted 07/29/2018
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: BMDM
    Verified Customer | Posted 03/28/2018
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: tusar giri
    Sample Tested: mice lung extract,mice lung protein
    Verified Customer | Posted 12/01/2017
  • Mouse IL-6 DuoSet ELISA
    Name: Ella Zeldich
    Sample Tested: mouse primary microglia cells
    Verified Customer | Posted 11/22/2017
    Excellent performance
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Peritoneal resident macrophages
    Verified Customer | Posted 09/27/2017
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 09/07/2017
  • Mouse IL-6 DuoSet ELISA
    Name: Shubhanchi Nigam
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 07/14/2017
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Lung tissue
    Verified Customer | Posted 05/02/2017
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 04/24/2017
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 04/18/2017
    Reliable results and easy to follow protocol
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 10/06/2016
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Skeletal muscle
    Verified Customer | Posted 09/30/2016
  • Mouse IL-6 DuoSet ELISA
    Name: Anonymous
    Sample Tested: mouse serum
    Verified Customer | Posted 08/04/2016
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA
    Name: I Sundar
    Sample Tested: mouse BAL fluid
    Verified Customer | Posted 07/05/2016
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA, 15 Plate
    Name: Jacqueline Kimmey
    Sample Tested: murine lung homogenate
    Verified Customer | Posted 04/08/2016
    consistent results, easy to use
    Mouse IL-6 DuoSet ELISA DY406
  • Mouse IL-6 DuoSet ELISA, 15 Plate
    Name: Anonymous
    Sample Tested: Purified Standard
    Verified Customer | Posted 10/26/2015
    50ul of murine IL-6 Capture antibody (2ug/mL) was coated per well of a 96 well plate at 4 degrees overnight. Purified mIL-6 standard was diluted (7 fold) and assayed to ensure the quality of the ELISA. Mouse IL-6 Detector antibody was added (2h @ RT) followed by a 20 min incubation with Streptavidin-HRP. Substrate solution was added to measure mIL-6 concentration according to the manufactures protocol. The standard curve looked great as can be seen by calculation of the R- squared value. Highly recommend doing ELISA's using kits from R&D. Buffer: 7-Fold dilution cruve was tried (only values that genrated a linear curve are shown)
    Mouse IL-6 DuoSet ELISA DY406

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Product Documents for Mouse IL-6 DuoSet ELISA

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