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Mouse IL-1 beta/IL-1F2 DuoSet ELISA

R&D Systems, Inc. a Bio-Techne Brand

Mouse IL-1 beta / IL-1F2 ELISA Standard Curve
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DY401-05
DY401

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Key Product Details

Assay Type: Solid Phase Sandwich ELISA
Assay Range: 15.6 - 1,000 pg/mL
Sample Types: Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Mouse IL-1 beta/IL-1F2 DuoSet ELISA Features:
  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Citations for Mouse IL-1 beta/IL-1F2 DuoSet ELISA (411)

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Customer Reviews for Mouse IL-1 beta/IL-1F2 DuoSet ELISA (29)

4.7 out of 5
29 customer ratings
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  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: PMBC
    Verified Customer | Posted 09/09/2022
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: BAL fluid
    Verified Customer | Posted 07/15/2022
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: whole blood supernatant
    Verified Customer | Posted 05/24/2022
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: J774A.1 mouse reticulum cell sarcoma macrophage cell line
    Verified Customer | Posted 03/03/2022
    Optimisation of cytokine response and cell numbers
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell Lysates
    Verified Customer | Posted 03/01/2022
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: BMDM supernates,Cell Culture Supernates,J774A.1 mouse reticulum cell sarcoma macrophage cell line
    Verified Customer | Posted 02/28/2022
    Except for the highest standard all other standards worked well
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: serum and cell supernatant
    Verified Customer | Posted 12/13/2021
    we have used this kit for LPS stimulated in vivo and in vitro experiments. It works very well and efficiently.
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Tissue Lysates
    Verified Customer | Posted 12/06/2021
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Martin Torres
    Sample Tested: Mice serum
    Verified Customer | Posted 09/23/2021
    Mouse IL-1b ELISA kit was used to assess IL-1b levels in serum after 12-weeks treatment of adult C57BL7/6J mice with a high-fat diet treatment. Results were accurate and showed low intra-experimental variation (less than 6 %). The picture shows the standard diet (SD), high-fat diet (HFD), and two concentrations of corn pericarp extract (200 and 500 parts per million of extract).
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Human cell conditioned medium
    Verified Customer | Posted 12/11/2020
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Mustafa Girnary
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Verified Customer | Posted 10/20/2020
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant,RAW 264.7 mouse monocyte/macrophage cell line
    Verified Customer | Posted 11/10/2019
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: mouse macrophages
    Verified Customer | Posted 09/11/2019
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Wen Li
    Sample Tested: Bone marrow-derived dendritic cells
    Verified Customer | Posted 08/06/2019
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Adult lung
    Verified Customer | Posted 05/20/2019
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Ivan Luzardo
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 12/11/2018
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Alejandra Palomino Antolín
    Sample Tested: Brain (glial cell in hippocampus)
    Verified Customer | Posted 07/27/2018
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: J774A.1 mouse reticulum cell sarcoma macrophage cell line
    Verified Customer | Posted 07/17/2018
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 07/13/2018
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Ella Zeldich
    Sample Tested: mouse primary microglia cells
    Verified Customer | Posted 11/22/2017
    We needed to calibrate the dilutions of our samples.
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Peritoneal resident macrophages
    Verified Customer | Posted 09/27/2017
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Brain tissue
    Verified Customer | Posted 07/21/2017
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Plasma
    Verified Customer | Posted 05/24/2017
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Whole blood
    Verified Customer | Posted 05/22/2017
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Lung tissue
    Verified Customer | Posted 04/19/2017
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Mouse plasma samples
    Verified Customer | Posted 07/05/2016
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA
    Name: Ariela Haimovich
    Sample Tested: BMDC cells stimulated with LPS for 4 hrs,Bone marrow-derived dendritic cells
    Verified Customer | Posted 05/24/2016
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA, 15 Plate
    Name: Jacqueline Kimmey
    Sample Tested: murine lung homogenate supernatant
    Verified Customer | Posted 04/05/2016
    lungs were homogenized in PBS-T, and sup was filtered through 0.22um filter before use. Homogenate was diluted 1:3 for analysis by ELISA.
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401
  • Mouse IL-1 beta/IL-1F2 DuoSet ELISA, 15 Plate
    Name: Anonymous
    Sample Tested: Purified mouse IL-1beta protein
    Verified Customer | Posted 10/26/2015
    50ul of murine IL-1beta Capture antibody (4ug/mL) was coated per well of a 96 well plate at 4 degrees overnight. Purified IL-1beta standard was diluted (7 fold) and assayed to ensure the quality of the ELISA. Mouse IL-1beta Detector antibody was added (2h @ RT) followed by a 20 min incubation with Streptavidin-HRP. Substrate solution was added to measure IL-1beta concentration according to the manufactures protocol. The standard curve looked great as can be seen by calculation of the R- squared value. Highly recommend doing ELISA's using kits from R&D. Buffer: 7-point standard curve
    Mouse IL-1 beta/IL-1F2 DuoSet ELISA DY401

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FAQs for Mouse IL-1 beta/IL-1F2 DuoSet ELISA

  • Does the Mouse IL-1 beta/IL-1F2 DuoSet ELISA (Catalog DY401) crosss-react with the pro-form of Mouse IL-1 beta?

    The Mouse IL-1 beta/IL-1F2 DuoSet ELISA is designed to detect mature mouse IL-1 beta and the assay is calibrated against the mature form of mouse IL-1 beta. It is possible that the kit also detects the pro-form, however, R&D Systems has not performed this characterization.

Product Documents for Mouse IL-1 beta/IL-1F2 DuoSet ELISA

Certificate of Analysis

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