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IL-10 Family Cytokines

R&D Systems Recombinant Human IL-10 Displays Higher Activity than a Top Competitor’s Recombinant Human IL-10 Protein

Comparison of the bioactivity of R&D Systems Recombinant Human IL-10 and a leading competitor’s IL-10 protein

R&D Systems Recombinant Human IL-10 Displays Higher Activity than a Leading Competitor’s Recombinant Human IL-10 Protein. MC/9-2 mouse mast cells were treated with increasing concentrations of R&D Systems Recombinant Human IL-10 (Catalog # 217-IL; red line) or with recombinant human IL-10 from a top competitor (blue line) and cell proliferation was assessed. The activity of the R&D Systems protein was approximately 2-fold greater than the competitor’s protein.

Protein Characterization Using SEC-MALS Analysis

A graph showing size exclusion chromatography multi-angle light scattering data for IL-10 recombinant protein

Recombinant Human IL‑10 Protein SEC-MALS. Recombinant human IL-10 (Catalog # 1064-ILB) has a molecular weight (MW) of 36.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

 

SEC-MALS Data Result
Retention Time 17.9-18.5 min
MW-Predicted (Monomer) 19.0 kDa
MW-MALS 36.7 kDa
Polydispersity 1.002
System Suitability: BSA Monomer 66.4 ± 3.32 kDa Pass

Detection of IL-10 R alpha in CD19+ Peripheral Blood Lymphocytes by Flow Cytometry

Flow cytometric detection of IL-10 R alpha in CD19+ human peripheral blood lymphocytes

Detection of IL-10 R alpha in Human Blood Lymphocytes by Flow Cyotmetry. Human peripheral blood lymphocytes were stained with a PE-conjugated Mouse Anti-Human CD19 Monoclonal Antibody (R&D Systems, Catalog # FAB4867P) and either (A) an Alexa Fluor® 647-conjugated Mouse Anti-Human IL-10 R alpha Monoclonal Antibody (R&D Systems, Catalog # FAB2742R) or (B) an Alexa Fluor 647-conjugated Mouse IgG2B Isotype Control Antibody (R&D Systems, Catalog # IC0041R).

Cell Proliferation Induced by R&D Systems Recombinant Human IL-19 and Neutralization by a Mouse Anti-Human IL-19 Monoclonal Antibody

Analysis of IL-19-induced proliferation of BaF3 mouse pro-B cells and neutralization by an IL-19 monoclonal antibody

IL-19-induced Cell Proliferation is Neutralized Using a Mouse Anti-Human IL-19 Monoclonal Antibody. The BaF3 mouse pro-B cell line co-transfected with human IL-20 R alpha and IL-20 R beta was treated with increasing concentrations of Recombinant Human IL-19 (R&D Systems, Catalog # 1035-IL) and cell proliferation was assessed (orange line). The ED50 for this effect is typically 0.2-1.2 ng/mL. Proliferation elicited by 3 ng/mL Recombinant Human IL-19 was neutralized by treating the cells with increasing concentrations of a Mouse Anti-Human IL-19 Monoclonal Antibody (R&D Systems, Catalog # MAB1035; blue line). The ND50 for this effect is typically 0.2-0.5 ug/mL.

Detection of CD4+IL-22+ Mouse Splenocytes by Flow Cytometry

Flow cytometric detection of IL-22 and CD4 in mouse splenocytes induced to develop into Th17 cells

Detection of IL-22 in Mouse Th17 Cells by Flow Cyotmetry. Mouse splenocytes differentiated to Th17 cells with plate-bound Rat Anti-Mouse CD3 epsilon Monoclonal Antibody (R&D Systems, Catalog # MAB484) plus Goat Anti-Mouse CD28 Polyclonal Antibody (R&D Systems, Catalog # AF483), Recombinant Human TGF-beta 1 (R&D Systems, Catalog # 240-B), Recombinant Mouse IL-23 (R&D Systems, Catalog # 1887-ML), Recombinant Mouse IL-6 (R&D Systems, Catalog # 406-ML), Recombinant Mouse IL-1 beta (R&D Systems, Catalog # 401-ML) and a Rat Anti-Mouse IFN-gamma Monoclonal Antibody (R&D Systems, Catalog # MAB485) for 5 days were stained with an APC-conjugated Rat Anti-Mouse CD4 Monoclonal Antibody (R&D Systems, Catalog # FAB554A) and either (A) a PE-conjugated Rat Anti-Mouse IL-22 Monoclonal Antibody (R&D Systems, Catalog # IC582P) or (B) a PE-conjugated Rat IgG2A Isotype Control Antibody (R&D Systems, Catalog # IC006P). To facilitate intracellular staining, cells were fixed and permeabilized using the FlowX FoxP3 Fixation and Permeabilization Buffer Kit (R&D Systems, Catalog # FC012).

IL-10 Secretion Induced by R&D Systems Recombinant Human IL-22 and Neutralization by a Goat Anti-Human IL-22 R alpha 1 Polyclonal Antibody

Analysis of IL-22-induced IL-10 secretion in COLO 205 cells and neutralization by an IL-22 R alpha 1 polyclonal antibody

IL-22-induced IL-10 Secretion is Neutralized Using a Goat Anti-Human IL-22 R alpha 1 Polyclonal Antibody. The COLO 205 human colorectal adenocarcinoma cell line was treated with increasing concentrations of Recombinant Human IL-22 (R&D Systems, Catalog # 782-IL) and IL-10 secretion was measured using the Human IL-10 DuoSet™ ELISA Development System (R&D Systems, Catalog # DY217B; orange line). The ED50 for this effect is typically 60-300 pg/mL. IL-10 secretion induced by 1 ng/mL Recombinant Human IL-22 was neutralized by treating the cells with increasing concentrations of a Goat Anti-Human IL-22 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (R&D Systems, Catalog # AF2770; blue line). The ND50 for this effect is typically 0.2-1.0 ug/mL.

Featured Products for IL-10 Family Research

Sandwich ELISA assay principle showing an antigen bound between specific capture and detection antibodies

Immunoassays for Detecting IL-10 Family Cytokines

From our complete, ready-to-use QuantikineTM ELISA Kits to our more flexible DuoSetTM ELISA Development Systems, we offer a wide selection of immunoassays for measuring IL-10 family cytokines. Whatever your needs, you can count on our immunoassays to deliver accurate, reproducible, high-quality data for every experimental sample that you test.

Proteome Profiler Teaser Solo

Proteome Profiler™ Human Phospho-Kinase Antibody Array

Explore signaling pathways activated downstream of IL-10 family cytokines using the Proteome Profiler Human Phospho-Kinase Array. This product offers a simple and inexpensive method to simultaneously assess the phosphorylation of 37 different kinases in a single sample, using chemiluminescence and Western blotting equipment.

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Bulk Proteins

If your experiments require large quantities of a particular protein, contact us for a bulk quote. We have the capacity and the expertise to scale up the production of any protein to meet your needs, and we offer economical pricing on bulk orders.

Featured IL-10 Family Resources

Illustration showing the ligand-receptor interactions for IL-10

Cytokine Signaling Pathways

Cytokines activate a diverse array of intracellular signaling pathways that can induce processes such as cell proliferation, differentiation, migration, and inflammation. Explore the signaling pathways that are activated by different cytokine families, the primary target cells that they affect, and the biological effects that they mediate using our interactive signaling pathways.

Periodic Table of Human Cytokine and Chemokine Poster Teaser Solo

Periodic Table of Cytokine & Chemokine Families Wall Poster

Learn about the members of different cytokine and chemokine families with our periodic table wall poster. This poster includes information about the structures, receptors, and native molecular weights of each cytokine, and will serve as a great reference tool and colorful addition to your lab space.

Thumbnail cover of Bio-Techne’s Cytokines and Proteins User’s Guide eBook

Cytokines and Proteins User’s Guide eBook

Download this guide to learn about the different applications for cytokines in cell biology research. From cell culture and lineage differentiation to antibody and immunoassay development, this guide highlights how recombinant proteins are being utilized in diverse research areas.