Recombinant Human IL-19 Protein

Name: Recombinant Human IL-19 Protein
Brand: R&D Systems
SKU: 1035-IL
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1035-IL

Citations (4)

(2)

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Key Product Details

Source:	E. coli
Accession #:	AAN40906
Applications:	Bioactivity

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Product Specifications for Recombinant Human IL-19 Protein

Source	E. coli-derived human IL-19 protein Leu25-Ala177, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	18 kDa (monomer)
Activity	Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED₅₀ for this effect is 0.2-1.2 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1035-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1035-IL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, 2 to 8 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-19

Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. Its gene contains two alternate translation initiation sites, generating precursors of 215 amino acids (aa) and 177 aa, respectively. Both isoforms are processed to 17 kDa, 153 aa mature molecules. IL-19 contains seven helices and is secreted as a 35 kDa monomer. There are two potential N-linked glycosylation sites, and it is likely that the molecule is glycosylated. Mature human IL-19 shares 69% aa sequence identity with the mature mouse homologue. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. IL-19 expression is limited to activated keratinocytes and monocytes. IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (IL-20 R alpha, also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R beta or IL-20 R2). This receptor complex is also shared by IL-20 and IL-24. Functionally, IL-19 induces IL-6 and TNF-alpha production by monocytes, and drives T-helper cell differentiation towards a Th2 response (1 - 5).

Item	Value
References	Gallagher, G. et al. (2000) Genes Immun. 1:442. Gallagher, G. et al. (2004) Int. Immunopharmacol. 4:615. Laio, Y-C. et al. (2002) J. Immunol. 169:4288. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
Long Name	Interleukin 19
Entrez Gene IDs	29949 (Human); 329244 (Mouse)
Alternate Names	IL-10C, IL-19, IL19, MDA1, NG.1Melanoma differentiation-associated protein-like protein, ZMDA1interleukin-19, interleukin 19, melanoma differentiation associated protein-like protein

Citations for Recombinant Human IL-19 Protein (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-19 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Robert Sabat (2016), MMP8 Is Increased in Lesions and Blood of Acne Inversa Patients: A Potential Link to Skin Destruction and Metabolic Alterations Mediators Inflamm., 2016(0):4097574.
PMID: 27843200
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tohyama M et al. (2009), IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression. Eur. J. Immunol., 39(10):2779-88.
PMID: 19731362
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Duluc D et al. (2009), Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages. Int. J. Cancer, 125(2):367-73.
PMID: 19378341
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Sa SM et al. (2007), The effects of IL-20 subfamily cytokines on reconstituted human epidermis suggest potential roles in cutaneous innate defense and pathogenic adaptive immunity in psoriasis. J. Immunol., 178(4):2229-40.
PMID: 17277128

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Customer Reviews for Recombinant Human IL-19 Protein (2)

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Recombinant Human IL-19 Protein

Name: Parinaz pircher

Application: FACS

Verified Customer | Posted 05/04/2020
Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 12/06/2018

Recombinant Human IL-19 Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Human IL-19 Protein

Formulation, Preparation and Storage

Carrier Free

1035-IL

Carrier

1035-IL/CF

Carrier Free

Reconstitution Calculator

Background: IL-19

Citations for Recombinant Human IL-19 Protein (4)

Customer Reviews for Recombinant Human IL-19 Protein (2)

Recombinant Human IL-19 Protein

Product Documents for Recombinant Human IL-19 Protein

Product Datasheets

COA

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