Human IL-19 Antibody

Name: Human IL-19 Antibody
Brand: R&D Systems
SKU: MAB1035

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1035

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human IL-19 Leu25-Ala177 Accession # AAN40906
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 152112
Specificity:	Detects human IL-19 in direct ELISAs.

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Applications for Human IL-19 Antibody

	Recommended Concentration	Sample
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/10⁶ cells	Human LPS-treated peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin

Item	Value
Neutralization	Measured by its ability to neutralize IL‑19-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND₅₀) is typically 0.2-0.5 µg/mL in the presence of 3 ng/mL Recombinant Human IL‑19.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-19 Antibody

	Neutralization Cell Proliferation Induced by IL‑19 and Neutralization by Human IL‑19 Antibody. Recombinant Human IL-19 (Catalog # 1035-IL) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-20 Ra and IL-20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-19 (3 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-19 Monoclonal Antibody (Catalog # MAB1035). The ND₅₀ is typically 0.2-0.5 µg/mL.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: IL-19

Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. Its gene contains two alternate translation initiation sites, generating precursors of 215 amino acids (aa) and 177 aa, respectively. Both isoforms are processed to 17 kDa, 153 aa mature molecules. IL-19 contains seven helices and is secreted as a 35 kDa monomer. There are two potential N-linked glycosylation sites, and it is likely that the molecule is glycosylated. Mature human IL-19 shares 69% aa sequence identity with the mature mouse homologue. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. IL-19 expression is limited to activated keratinocytes and monocytes. IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (IL-20 R alpha, also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R beta or IL-20 R2). This receptor complex is also shared by IL-20 and IL-24. Functionally, IL-19 induces IL-6 and TNF-alpha production by monocytes, and drives T-helper cell differentiation towards a Th2 response (1‑5).

Item	Value
References	Gallagher, G. et al. (2000) Genes Immun. 1:442. Gallagher, G. et al. (2004) Int. Immunopharmacol. 4:615. Laio, Y-C. et al. (2002) J. Immunol. 169:4288. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
Long Name	Interleukin 19
Entrez Gene IDs	29949 (Human); 329244 (Mouse)
Alternate Names	IL-10C, IL-19, IL19, MDA1, NG.1Melanoma differentiation-associated protein-like protein, ZMDA1interleukin-19, interleukin 19, melanoma differentiation associated protein-like protein

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Human IL-19 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Scientific Data Examples for Human IL-19 Antibody

Neutralization

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Background: IL-19

Citations for Human IL-19 Antibody

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