Human IL-19 Alexa Fluor® 405-conjugated Antibody

Name: Human IL-19 Alexa Fluor® 405-conjugated Antibody
Brand: R&D Systems
SKU: IC1035V

R&D Systems, Inc. a Bio-Techne Brand

Catalog # IC1035V

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Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human IL-19 Leu25-Ala177 Accession # AAN40906
Applications:	Flow Cytometry
Label:	Alexa Fluor 405 (Excitation=405 nm, Emission=421 nm)
Specificity:	Detects human IL-19 in direct ELISAs.

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Applications for Human IL-19 Alexa Fluor® 405-conjugated Antibody

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	0.25-1 µg/10⁶ cells	Human LPS-treated peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Formulation	Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

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Background: IL-19

Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. Its gene contains two alternate translation initiation sites, generating precursors of 215 amino acids (aa) and 177 aa, respectively. Both isoforms are processed to 17 kDa, 153 aa mature molecules. IL-19 contains seven helices and is secreted as a 35 kDa monomer. There are two potential N-linked glycosylation sites, and it is likely that the molecule is glycosylated. Mature human IL-19 shares 69% aa sequence identity with the mature mouse homologue. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. IL-19 expression is limited to activated keratinocytes and monocytes. IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (IL-20 R alpha, also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R beta or IL-20 R2). This receptor complex is also shared by IL-20 and IL-24. Functionally, IL-19 induces IL-6 and TNF-alpha production by monocytes, and drives T-helper cell differentiation towards a Th2 response (1‑5).

Item	Value
References	Gallagher, G. et al. (2000) Genes Immun. 1:442. Gallagher, G. et al. (2004) Int. Immunopharmacol. 4:615. Laio, Y-C. et al. (2002) J. Immunol. 169:4288. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
Long Name	Interleukin 19
Entrez Gene IDs	29949 (Human); 329244 (Mouse)
Alternate Names	IL-10C, IL-19, IL19, MDA1, NG.1Melanoma differentiation-associated protein-like protein, ZMDA1interleukin-19, interleukin 19, melanoma differentiation associated protein-like protein

Product Specific Notices for Human IL-19 Alexa Fluor® 405-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Human IL-19 Alexa Fluor® 405-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human IL-19 Alexa Fluor® 405-conjugated Antibody

Formulation, Preparation and Storage

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Background: IL-19

Product Specific Notices for Human IL-19 Alexa Fluor® 405-conjugated Antibody

Citations for Human IL-19 Alexa Fluor® 405-conjugated Antibody

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