Recombinant Mouse IL-23 Protein Best Seller

Catalog # 1887-ML | R&D Systems, Inc. a Bio-Techne Brand
Analyzed by SEC-MALS. Sf21-Expressed
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Carrier Free
1887-ML-010/CF
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With Carrier
1887-ML-010
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Key Product Details

Accession #

P43432 (p40) & Q9EQ14 (p19)

Source

Sf 21 (stably transfected)

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse IL-23 protein
Mouse IL-23 p40
(Met23-Ser335)
Accession # P43432		 IGSGSSRGGSGSGGSGGGGSK Mouse IL-23 p19
(Leu20-Ala196)
Accession # Q9EQ14
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met23

Predicted Molecular Mass

57 kDa

SDS-PAGE

(58-71) kDa, non-reducing conditions

Activity

Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
The ED50 for this effect is 0.05-0.25 ng/mL.

Scientific Data Images for Recombinant Mouse IL-23 Protein

Recombinant Mouse IL‑23 Protein SEC-MALS.

Recombinant Mouse IL-23 (Catalog # 1887-ML) has a molecular weight (MW) of 60.4 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1887-ML
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1887-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-23

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human Tnbsp;cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

References

  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5699.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Long Name

Interleukin 23

Alternate Names

IL-23 p19/IL-12 p40, IL-23p19/IL-12p40, IL23, SGRF

Entrez Gene IDs

51561 (Human); 83430 (Mouse); 155140 (Rat); 102128555 (Cynomolgus Monkey)

Gene Symbol

IL23A

UniProt

P43432 (p40) & Q9EQ14 (p19)

Product Documents for Recombinant Mouse IL-23 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 1887-ML (with carrier)
Download SDS 1887-ML/CF (carrier free)

Product Specific Notices for Recombinant Mouse IL-23 Protein

For research use only

Citations for Recombinant Mouse IL-23 Protein (180)

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Customer Reviews for Recombinant Mouse IL-23 Protein (5)

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  • Recombinant Mouse IL-23 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 05/23/2020
    Recombinant Mouse IL-23 Protein 1887-ML
  • Recombinant Mouse IL-23 Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 03/17/2020
    Recombinant Mouse IL-23 Protein 1887-ML
  • Recombinant Mouse IL-23 Protein
    Name: Anonymous
    Application: Glycosylation characterization by MS
    Verified Customer | Posted 02/07/2018
    Recombinant Mouse IL-23 Protein 1887-ML
  • Recombinant Mouse IL-23 Protein
    Name: Anonymous
    Application: CD4+ T Cell polarization toward Th17
    Verified Customer | Posted 08/24/2017
    Recombinant Mouse IL-23 Protein 1887-ML
  • Recombinant Mouse IL-23 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 08/24/2016
    Recombinant Mouse IL-23 Protein 1887-ML

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FAQs for Recombinant Mouse IL-23 Protein

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  • Q: Should I use Recombinant Mouse IL-23 Protein formulated with carrier BSA (Catalog # 1887-ML) or the Recombinant Mouse IL-23 Protein, CF (Catalog # 1887-ML/CF) that is carrier-free (no BSA) for my in vivo experiments?

    A: For in vivo aplications, we would recommend using the carrier-free Mouse IL-23 Protein (Catalog # 1887-ML/CF). The injection of a carrier protein such as BSA can cause side effects and sometimes death to the experimental animal. For more information regarding use of the protein for in vivo applications, we would recommend consulting the Citations tab on the product-specific page.

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