Mouse CD28 Antibody
Catalog # AF483 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
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Product Summary for Mouse CD28 Antibody
Immunogen
Lys21-Lys149
Accession # P31041
Specificity
Clonality
Host
Isotype
Applications for Mouse CD28 Antibody
Western Blot
Sample: Recombinant Mouse CD28 Fc Chimera (Catalog # 483-CD)
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD28
CD28 and CTLA-4, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig V‑like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is upregulated rapidly following T cell activation and CD28 ligation. Cell surface expression of CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7‑1 and B7‑2 with a 20‑100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up-regulation of bcl‑XL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation.
Product Documents for Mouse CD28 Antibody
Product Specific Notices for Mouse CD28 Antibody
For research use only
Citations for Mouse CD28 Antibody (2)
Citations are publications that use Bio-Techne products. Selected citations for Mouse CD28 Antibody include:
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Species: Mouse
Sample Types: Whole Cells
Applications: BioassayE Engelowski et al. (2018-11-20), IL-23R Signaling Plays No Role in Myocardial Infarction Sci Rep, 2018-18(1):17078.
PMID: 30459442 -
Species: Mouse
Sample Types: Recombinant Protein
Applications: Western BlotChemnitz JM et al. (2006-06-01), B and T lymphocyte attenuator-mediated signal transduction provides a potent inhibitory signal to primary human CD4 T cells that can be initiated by multiple phosphotyrosine motifs. J. Immunol., 2006-0176(11):6603-14.
PMID: 16709818
There are no citations that match your criteria.
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.