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Mouse CD28 Antibody
Catalog # AF483 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Summary for Mouse CD28 Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD28
Lys21-Lys149
Accession # P31041
Lys21-Lys149
Accession # P31041
Specificity
Detects mouse CD28 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human CD28 and recombinant mouse CTLA-4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse CD28 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CD28 Fc Chimera (Catalog # 483-CD)
Sample: Recombinant Mouse CD28 Fc Chimera (Catalog # 483-CD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD28
CD28 and CTLA-4, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig V‑like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is upregulated rapidly following T cell activation and CD28 ligation. Cell surface expression of CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7‑1 and B7‑2 with a 20‑100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up-regulation of bcl‑XL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation.
Product Documents for Mouse CD28 Antibody
Product Specific Notices for Mouse CD28 Antibody
For research use only
Citations for Mouse CD28 Antibody (2)
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Citations are publications that use Bio-Techne products. Selected citations for Mouse CD28 Antibody include:
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Species: Mouse
Sample Types: Whole Cells
Applications: BioassayE Engelowski et al. (2018-11-20), IL-23R Signaling Plays No Role in Myocardial Infarction Sci Rep, 2018-18(1):17078.
PMID: 30459442 -
Species: Mouse
Sample Types: Recombinant Protein
Applications: Western BlotChemnitz JM et al. (2006-06-01), B and T lymphocyte attenuator-mediated signal transduction provides a potent inhibitory signal to primary human CD4 T cells that can be initiated by multiple phosphotyrosine motifs. J. Immunol., 2006-0176(11):6603-14.
PMID: 16709818
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