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Recombinant Mouse IL-6 Protein Best Seller

Bio-Techne includes R&D Systems | Catalog # 406-ML

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Carrier Free
406-ML-005/CF
406-ML-025/CF

Catalog #
Availability
Size / Price
Qty
With Carrier
406-ML-005
406-ML-025

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived mouse IL-6 protein
Phe25-Thr211, with an N-terminal Met

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe25

Predicted Molecular Mass

21.8 kDa

Activity

Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813.
The ED50 for this effect is 10.0-100 pg/mL.

Scientific Data Images for Recombinant Mouse IL-6 Protein

Recombinant Mouse IL-6 Protein Bioactivity

Recombinant Mouse IL-6 Protein Bioactivity

Recombinant Mouse IL-6 (Catalog # 406‑ML) stimulates cell proliferation of the T1165.85.2.1 mouse plasmacytoma cell line. The ED50 for this effect is 10.0-100 pg/mL.
Recombinant Mouse IL-6 Protein SDS-PAGE

Recombinant Mouse IL-6 Protein SDS-PAGE

1 μg/lane of Recombinant Mouse IL-6 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 24 kDa.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 406-ML
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Acetate and EDTA with Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 406-ML/CF
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate and EDTA.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha-helical, 22 - 28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1 - 5). Mature mouse IL-6 is 187 amino acids (aa) in length and shares 39% and 85% aa sequence identity with human and rat IL-6, respectively (6 - 8). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (9). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (10). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 11). IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1 - 5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1, 2, 5). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 12).

References

  1. Mansell, A. and B.J. Jenkins (2013) Cytokine Growth Factor Rev. 24:249.
  2. Schuett, H. et al. (2009) Thromb. Haemost. 102:215.
  3. Erta, M. et al. (2012) Int. J. Biol. Sci. 8:1254.
  4. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
  5. Mihara, M. et al. (2012) Clin. Sci. (Lond.) 122:143.
  6. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7099.
  7. Simpson, R.J. et al. (1988) Eur. J. Biochem. 176:187.
  8. Van Snick, J. et al. (1988) Eur. J. Immunol. 18:193.
  9. Murakami, M. et al. (1993) Science 260:1808.
  10. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
  11. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
  12. Cerutti, A. et al. (1998) J. Immunol. 160:2145.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)

Gene Symbol

IL6

UniProt

Additional IL-6 Products

Product Documents for Recombinant Mouse IL-6 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IL-6 Protein

For research use only

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