Recombinant Human IL-10 Protein

Name: Recombinant Human IL-10 Protein
Brand: R&D Systems
SKU: 217-IL
Rating: 5 (8 reviews)

Catalog # 217-IL | R&D Systems, Inc. a Bio-Techne Brand

(8)

Citations (105)

Product Datasheet / COA / SDS

New Version Available - 1064-ILB

Analyzed by SEC-MALS. E. Coli Derived Version Also Available

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Setting the standard in quality research reagents

Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	P22301
Structure / Form:	Noncovalently-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-10 Protein

Product Specifications for Recombinant Human IL-10 Protein

Source	Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-10 protein Ser19-Asn178
Purity	>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser19
Predicted Molecular Mass	18.6 kDa
Activity	Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED₅₀ for this effect is 0.075-0.750 ng/mL.

Scientific Data Examples for Recombinant Human IL-10 Protein

	Recombinant Human IL‑10 Protein SEC-MALS. Recombinant human IL-10 (Catalog # 217-IL) has a molecular weight (MW) of 40.4 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
	Recombinant Human IL-10 Protein Bioactivity Recombinant Human IL-10 (Catalog # 217-IL) stimulates cell proliferation of MC/9-2 mouse mast cells. The activity is approximately 2-fold greater than the top competitor's Recombinant Human IL-10.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

217-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

217-IL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 50 µg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-10

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL‑10 family of alpha ‑helical cytokines that also includes
IL‑19, IL‑20, IL‑22, IL‑24, and IL‑26/AK155 (1, 2). IL‑10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature human IL‑10 shares 72%‑86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL‑10. Whereas human IL‑10 is active on mouse cells, mouse IL‑10 does not act on human cells (6, 7). IL‑10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (6, 8, 9). The IL‑10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL‑10 R beta /IL‑10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (10). IL‑10 R beta does not bind IL‑10 by itself but is required for signal transduction (1). IL‑10 R beta also associates with IL‑20 R alpha, IL‑22R alpha, or IL‑28 R alpha to form the receptor complexes for IL‑22, IL‑26, IL‑28, and IL‑29
(11‑13). IL‑10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (14‑16). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

Item	Value
References	Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. Sabat, R. et al. (2010) Cytokine Growth Factor Rev. 21:331. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172. Hsu, D.-H. et al. (1990) Science 250:830. Windsor, W.T. et al. (1993) Biochemistry 32:8807. Syto, R. et al. (1998) Biochemistry 37:16943. Kotenko, S.V. et al. (1997) EMBO J. 16:5894. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725. Hor, S. et al. (2004) J. Biol. Chem. 279:33343. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269. Blackburn, S.D. and E.J. Wherry (2007)Trends Microbiol. 15:143.
Long Name	Interleukin 10
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Alternate Names	CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL-10, IL-10MGC126451, IL10, IL10A, TGIF, interleukin 10, interleukin-10

Citations for Recombinant Human IL-10 Protein (105)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-10 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

H Asashima et al. (2023-01-02), PD-1highCXCR5-CD4+ peripheral helper Tï¿½cells promote CXCR3+ plasmablasts in human acute viral infection Cell Reports, 2023-00(0):111895.
PMID: 36596303
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Gunawardan et al. (2021-01-21), Tissue-specific endothelial cell heterogeneity contributes to unequal inflammatory responses Scientific Reports, 2021-011(1):1949.
PMID: 33479269
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

P Shrestha et al. (2021-01-07), Pomalidomide restores immune recognition of primary effusion lymphoma through upregulation of ICAM-1 and B7-2 PloS Pathogens, 2021-017(1):e1009091.
PMID: 33411730
Species: Human
Sample Types: Spheroid
Applications: Spheroid Culture

J Stebbing et al. (2021-01-01), JAK inhibition reduces SARS-CoV-2 liver infectivity and modulates inflammatory responses to reduce morbidity and mortality Sci Adv, 2021-00(0).
PMID: 33187978
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

J Chen et al. (2020-12-10), Kdm6a suppresses the alternative activation of macrophages and impairs energy expenditure in obesity Cell Death and Differentiation, 2020-10(0).
PMID: 33303977
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MY Hsieh et al. (2020-05-29), Diminished toll-like receptor response in febrile infection-related epilepsy syndrome (FIRES) Biomed J, 2020-00(0).
PMID: 32651134
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MF Abas?yan?k et al. (2020-05-25), Ultrasensitive digital quantification of cytokines and bacteria predicts septic shock outcomes Nat Commun, 2020-011(1):2607.
PMID: 32451375
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Sun et al. (2020-03-13), IL-10 Dampens an IL-17-Mediated Periodontitis-Associated Inflammatory Network J. Immunol., 2020-0204(8):2177-2191.
PMID: 32169848
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Li et al. (2019-11-06), Anti-inflammatory cytokines IL-35 and IL-10 block atherogenic lysophosphatidylcholine-induced, mitochondrial ROS-mediated innate immune activation, but spare innate immune memory signature in endothelial cells Redox Biol, 2019-128(0):101373.
PMID: 31731100
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

N Ogasawara et al. (2019-10-22), Role of RANK-L as a potential inducer of ILC2-mediated type 2 inflammation in chronic rhinosinusitis with nasal polyps Mucosal Immunol, 2019-113(1):86-95.
PMID: 31641233
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

ER Wonderlich et al. (2019-10-14), Effector memory differentiation increases detection of replication-competent HIV-l in resting CD4+ T cells from virally suppressed individuals PLoS Pathog., 2019-115(10):e1008074.
PMID: 31609991
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SK Horrevorts et al. (2019-08-28), Glycan-Modified Melanoma-Derived Apoptotic Extracellular Vesicles as Antigen Source for Anti-Tumor Vaccination Cancers (Basel), 2019-011(9).
PMID: 31466401
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Ye et al. (2019-04-11), Iguratimod represses B cell terminal differentiation linked with the inhibition of PKC/EGR1 axis Arthritis Res. Ther., 2019-021(1):92.
PMID: 30971291
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

BA Kane et al. (2019-02-04), Differential expression and regulation of the non-integrin 37/67-kDa laminin receptor on peripheral blood leukocytes of healthy individuals and patients with rheumatoid arthritis Sci Rep, 2019-09(1):1149.
PMID: 30718719
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

F Charbit-He et al. (2018-10-26), Copy number variations and founder effect underlying complete IL-10R? deficiency in Portuguese kindreds PLoS ONE, 2018-113(10):e0205826.
PMID: 30365510
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

AS Hansen et al. (2018-07-04), CD46 activation induces distinct CXCL-10 response in monocytes and monocyte-derived dendritic cells Cytokine, 2018-00(0).
PMID: 29980471
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

EW Kim et al. (2018-07-02), Vitamin D status contributes to the antimicrobial activity of macrophages against Mycobacterium leprae PLoS Negl Trop Dis, 2018-012(7):e0006608.
PMID: 29965969
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SL Jaslow et al. (2018-06-19), Salmonella Activation of STAT3 Signaling by SarA Effector Promotes Intracellular Replication and Production of IL-10 Cell Rep, 2018-023(12):3525-3536.
PMID: 29924996
Species: Human
Sample Types: Whole Cells
Applications: Differentiation, Differentiation

MW Tang et al. (2018-05-01), Class 3 semaphorins modulate the invasive capacity of rheumatoid arthritis fibroblast-like synoviocytes Rheumatology (Oxford), 2018-00(0).
PMID: 29471421
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MK Hasan et al. (2018-04-20), Wnt5a induces ROR1 to recruit DOCK2 to activate Rac1/2 in chronic lymphocytic leukemia Blood, 2018-00(0).
PMID: 29678828
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

E Chen et al. (2018-04-09), Concentration-dependent, dual roles of IL-10 in the osteogenesis of human BMSCs via P38/MAPK and NF-ÎºB signaling pathways FASEB J., 2018-00(0):fj201701256RR.
PMID: 29630408
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JG Chen et al. (2018-03-01), BTKCys481Serdrives ibrutinib resistance via ERK1/2, and protects BTKWild-TypeMYD88 mutated cells by a paracrine mechanism Blood, 2018-00(0).
PMID: 29496671
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Palazzo et al. (2018-02-16), The PI3K?-Selective Inhibitor Idelalisib Minimally Interferes with Immune Effector Function Mediated by Rituximab or Obinutuzumab and Significantly Augments B Cell Depletion In Vivo J. Immunol., 2018-00(0).
PMID: 29453281
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

U Gopinathan et al. (2018-01-19), Large-scale reduction of tyrosine kinase activities in human monocytes stimulated in vitro with N. meningitidis PLoS ONE, 2018-013(1):e0181912.
PMID: 29357362
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

RHP Wilbers et al. (2017-10-10), Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain PLoS ONE, 2017-112(10):e0186317.
PMID: 29016674
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Li et al. (2017-03-06), Interleukin 2 and interleukin 10 function synergistically to promote CD8(+) T cell cytotoxicity, which is suppressed by regulatory T cells in breast cancer Int. J. Biochem. Cell Biol, 2017-00(0).
PMID: 28274688
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Anja Kerstein (2016-12-28), Environmental factor and inflammation-driven alteration of the total peripheral T-cell compartment in granulomatosis with polyangiitis J. Autoimmun, 2016-10(0).
PMID: 28040323
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-12-19), Macrophages Enhance Migration in Inflammatory Breast Cancer Cells via RhoC GTPase Signaling Sci Rep, 2016-16(0):39190.
PMID: 27991524
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

David M Aronoff (2016-11-15), Lipid profiling of polarized human monocyte-derived macrophages Prostaglandins Other Lipid Mediat., 2016-1127(0):1-8.
PMID: 27871801
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-11-09), PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells Sci Rep, 2016-16(0):36795.
PMID: 27827428
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-11-03), Imprime PGG-Mediated Anti-Cancer Immune Activation Requires Immune Complex Formation PLoS ONE, 2016-111(11):e0165909.
PMID: 27812183
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-10-05), Tyrosine kinase 2 is not limiting human antiviral type III interferon responses Eur J Immunol, 2016-10(0).
PMID: 27615517
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kim H et al. (2015-04-01), Human umbilical cord blood mesenchymal stem cell-derived PGE2 and TGF-beta1 alleviate atopic dermatitis by reducing mast cell degranulation. Stem Cells, 2015-033(4):1254-66.
PMID: 25522163
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Andreani G et al. (2015-02-24), Leishmania infantum amastigotes trigger a subpopulation of human B cells with an immunoregulatory phenotype. PLoS Negl Trop Dis, 2015-09(2):e0003543.
PMID: 25710789
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Reiner A et al. (2014-12-31), Motor, visual and emotional deficits in mice after closed-head mild traumatic brain injury are alleviated by the novel CB2 inverse agonist SMM-189. Int J Mol Sci, 2014-116(1):758-87.
PMID: 25561230
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wang J et al. (2014-12-09), Novel mechanism of macrophage-mediated metastasis revealed in a zebrafish model of tumor development. Cancer Res, 2014-175(2):306-15.
PMID: 25492861
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Karahan G et al. (2014-07-01), Polyclonal B cell activation for accurate analysis of pre-existing antigen-specific memory B cells. Clin Exp Immunol, 2014-0177(1):333-40.
PMID: 24611883
Species: Hamster
Sample Types: Whole Cells
Applications: Bioassay

Osorio E et al. (2014-06-26), Growth factor and Th2 cytokine signaling pathways converge at STAT6 to promote arginase expression in progressive experimental visceral leishmaniasis. PLoS Pathog, 2014-010(6):e1004165.
PMID: 24967908
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Cheng et al. (2014-05-19), Cluster of differentiation 45 activation is crucial in interleukin-10-dependent tumor-associated dendritic cell differentiation. Oncol Lett, 2014-08(2):620-626.
PMID: 25013476
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay

Anuradha R et al. (2014-04-03), Expansion of parasite-specific CD4+ and CD8+ T cells expressing IL-10 superfamily cytokine members and their regulation in human lymphatic filariasis. PLoS Negl Trop Dis, 2014-08(4):e2762.
PMID: 24699268
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

O'Reilly S et al. (2014-02-18), Interleukin-6 (IL-6) trans signaling drives a STAT3-dependent pathway that leads to hyperactive transforming growth factor-beta (TGF-beta) signaling promoting SMAD3 activation and fibrosis via Gremlin protein. J Biol Chem, 2014-0289(14):9952-60.
PMID: 24550394
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Valle Oseguera et al. (2014-02-10), cmvIL-10 stimulates the invasive potential of MDA-MB-231 breast cancer cells. PLoS ONE, 2014-09(2):e88708.
PMID: 24520416
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Italiani et al. (2014-02-03), Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro. PLoS ONE, 2014-09(2):e87680.
PMID: 24498352
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Du M et al. (2014-01-22), Embryonic trophoblasts induce decidual regulatory T cell differentiation and maternal-fetal tolerance through thymic stromal lymphopoietin instructing dendritic cells. J Immunol, 2014-0192(4):1502-11.
PMID: 24453244
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

McCutcheon et al. (2014-01-08), Multiplexed screening of natural humoral immunity identifies antibodies at fine specificity for complex and dynamic viral targets. MAbs, 2014-06(2):460-73.
PMID: 24492306
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Suzuki et al. (2014-01-07), Cytokines alter IgA1 O-glycosylation by dysregulating C1GalT1 and ST6GalNAc-II enzymes. J Biol Chem, 2014-0289(8):5330-9.
PMID: 24398680
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Garcia S et al. (2014-01-03), Tie2 signaling cooperates with TNF to promote the pro-inflammatory activation of human macrophages independently of macrophage functional phenotype. PLoS ONE, 2014-09(1):e82088.
PMID: 24404127
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hamada et al. (2014-01-01), MafB promotes atherosclerosis by inhibiting foam-cell apoptosis. Nat Commun, 2014-05(0):3147.
PMID: 24445679
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gonzalez-Lombana C et al. (2013-03-21), IL-17 mediates immunopathology in the absence of IL-10 following Leishmania major infection. PLoS Pathog, 2013-09(3):e1003243.
PMID: 23555256
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Verjee L et al. (2013-02-19), Unraveling the signaling pathways promoting fibrosis in Dupuytren's disease reveals TNF as a therapeutic target. Proc Natl Acad Sci U S A, 2013-0110(10):E928-37.
PMID: 23431165
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Mazerolles F et al. (2013-02-10), Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency. J Allergy Clin Immunol, 2013-0131(4):1146-56, 1156.
PMID: 23403044
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Johnston A et al. (2013-01-31), Susceptibility-associated genetic variation at IL12B enhances Th1 polarization in psoriasis. Hum Mol Genet, 2013-022(9):1807-15.
PMID: 23376980
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Martinez-Gallo M et al. (2012-12-11), TACI mutations and impaired B-cell function in subjects with CVID and healthy heterozygotes. J Allergy Clin Immunol, 2012-1131(2):468-76.
PMID: 23237420
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Huse K et al. (2012-10-01), Role of Smad proteins in resistance to BMP-induced growth inhibition in B-cell lymphoma. PLoS ONE, 2012-17(10):e46117.
PMID: 23049692
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Corvaisier M et al. (2012-09-25), IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation. PLoS Biol, 2012-010(9):e1001395.
PMID: 23055831
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2012-09-17), Macrophages induce differentiation of plasma cells through CXCL10/IP-10. J. Exp. Med., 2012-0209(10):1813-23.
PMID: 22987802
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Graham LM et al. (2012-06-11), The C-type Lectin Receptor CLECSF8 (CLEC4D) Is Expressed by Myeloid Cells and Triggers Cellular Activation through Syk Kinase. J. Biol. Chem., 2012-0287(31):25964-74.
PMID: 22689578
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Mujib S et al. (2012-03-14), Antigen-independent induction of Tim-3 expression on human T cells by the common gamma-chain cytokines IL-2, IL-7, IL-15, and IL-21 is associated with proliferation and is dependent on the phosphoinositide 3-kinase pathway. J. Immunol., 2012-0188(8):3745-56.
PMID: 22422881
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Liu X et al. (2010-11-08), IFN-alpha blocks IL-17 production by peripheral blood mononuclear cells in Behcet's disease. Rheumatology (Oxford), 2010-150(2):293-8.
PMID: 21059671
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kalliolias GD et al. (2010-10-22), Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27. J. Immunol., 2010-1185(11):7047-56.
PMID: 20971923
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ghigo E et al. (2010-10-21), IL-16 promotes T. whipplei replication by inhibiting phagosome conversion and modulating macrophage activation. PLoS ONE, 2010-15(10):e13561.
PMID: 21042409
Species: Mouse, Rat
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo

Soderquist RG et al. (2010-06-01), PEGylation of interleukin-10 for the mitigation of enhanced pain states. J Biomed Mater Res A, 2010-093(3):1169-79.
PMID: 19768789
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ghirelli C et al. (2010-04-09), Systematic cytokine receptor profiling reveals GM-CSF as a novel TLR-independent activator of human plasmacytoid predendritic cells. Blood, 2010-0115(24):5037-40.
PMID: 20382843
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Liang X et al. (2010-03-25), Toll-like receptor 9 signaling by CpG-B oligodeoxynucleotides induces an apoptotic pathway in human chronic lymphocytic leukemia B cells. Blood, 2010-0115(24):5041-52.
PMID: 20339095
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Jindra C (2010-02-01), Human rhinoviruses induce IL-35-producing Treg via induction of B7-H1 (CD274) and sialoadhesin (CD169) on DC. Eur J Immunol, 2010-040(2):321-9.
PMID: 19950173
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Maarof G et al. (2009-11-24), Interleukin-24 inhibits the plasma cell differentiation program in human germinal center B cells. Blood, 2009-1115(9):1718-26.
PMID: 19965640
Sample Types: N/A
Applications: ELISA (Standard)

Heo YJ et al. (2009-11-04), IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients. Immunol. Lett., 2009-1127(2):150-6.
PMID: 19895848
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Park-Min KH et al. (2009-07-22), IL-10 suppresses calcium-mediated costimulation of receptor activator NF-kappa B signaling during human osteoclast differentiation by inhibiting TREM-2 expression. J. Immunol., 2009-0183(4):2444-55.
PMID: 19625651
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Chang WL et al. (2009-06-13), Human cytomegalovirus suppresses type I interferon secretion by plasmacytoid dendritic cells through its interleukin 10 homolog. Virology, 2009-0390(2):330-7.
PMID: 19524994
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Shahrara S et al. (2009-03-15), IL-17 induces monocyte migration in rheumatoid arthritis. J. Immunol., 2009-0182(6):3884-91.
PMID: 19265168
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kinoshita H et al. (2008-12-03), Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA. J. Allergy Clin. Immunol., 2008-1123(1):179-86.
PMID: 19056108
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hirosako S et al. (2008-11-26), Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells. Clin. Exp. Immunol., 2008-1155(2):266-74.
PMID: 19040600
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Rojahn A et al. (2008-09-01), Does granulocyte colony-stimulating factor ameliorate the proinflammatory response in human meningococcal septic shock? Crit. Care Med., 2008-036(9):2583-9.
PMID: 18679115
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hollins F et al. (2008-08-15), Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6. J. Immunol., 2008-0181(4):2772-80.
PMID: 18684968
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Moir S et al. (2008-07-14), Evidence for HIV-associated B cell exhaustion in a dysfunctional memory B cell compartment in HIV-infected viremic individuals. J. Exp. Med., 2008-0205(8):1797-805.
PMID: 18625747
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

van der Vliet HJ et al. (2008-06-01), Circulating myeloid dendritic cells of advanced cancer patients result in reduced activation and a biased cytokine profile in invariant NKT cells. J. Immunol., 2008-0180(11):7287-93.
PMID: 18490728
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kato A et al. (2008-04-14), Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps. J. Allergy Clin. Immunol., 2008-0121(6):1385-92, 1392.
PMID: 18410958
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Liu TY et al. (2008-04-01), Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Eur. J. Immunol., 2008-038(4):1012-23.
PMID: 18350544
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Scaldaferri F et al. (2007-07-01), Crucial role of the protein C pathway in governing microvascular inflammation in inflammatory bowel disease. J. Clin. Invest., 2007-0117(7):1951-60.
PMID: 17557119
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ettinger R et al. (2007-03-01), IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells. J. Immunol., 2007-0178(5):2872-82.
PMID: 17312131
Sample Types: N/A
Applications: ELISA (Standard)

Biancotto A et al. (2007-02-08), Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 2007-0109(10):4272-9.
PMID: 17289812
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gleissner CA et al. (2007-01-01), IL-10 inhibits endothelium-dependent T cell costimulation by up-regulation of ILT3/4 in human vascular endothelial cells. Eur. J. Immunol., 2007-037(1):177-92.
PMID: 17163451
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wan B et al. (2006-12-15), Aberrant regulation of synovial T cell activation by soluble costimulatory molecules in rheumatoid arthritis. J. Immunol., 2006-1177(12):8844-50.
PMID: 17142787
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Venet F et al. (2006-11-01), Human CD4+CD25+ regulatory T lymphocytes inhibit lipopolysaccharide-induced monocyte survival through a Fas/Fas ligand-dependent mechanism. J. Immunol., 2006-1177(9):6540-7.
PMID: 17056586
Sample Types: N/A
Applications: ELISA (Standard)

Du Z et al. (2006-04-15), Selective regulation of IL-10 signaling and function by zymosan. J. Immunol., 2006-0176(8):4785-92.
PMID: 16585572
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hsieh FH et al. (2005-09-26), Human airway epithelial cell determinants of survival and functional phenotype for primary human mast cells. Proc. Natl. Acad. Sci. U.S.A., 2005-0102(40):14380-5.
PMID: 16186496
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Nagineni CN et al. (2005-04-01), Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta. J. Cell. Physiol., 2005-0203(1):35-43.
PMID: 15368539
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Fujitsu Y et al. (2005-03-01), Protection of human conjunctival fibroblasts from NO-induced apoptosis by interleukin-4 or interleukin-13. Invest. Ophthalmol. Vis. Sci., 2005-046(3):797-802.
PMID: 15728533
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Pound JD et al. (2005-03-01), Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma. J. Immunol., 2005-0174(5):3015-23.
PMID: 15728515
Sample Types: N/A
Applications: ELISA (Standard)

Mozaffarian D et al. (2004-12-01), trans fatty acids and systemic inflammation in heart failure. Am. J. Clin. Nutr., 2004-180(6):1521-5.
PMID: 15585763
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gibot S et al. (2004-11-22), A soluble form of the triggering receptor expressed on myeloid cells-1 modulates the inflammatory response in murine sepsis. J. Exp. Med., 2004-1200(11):1419-26.
PMID: 15557347
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kao CY et al. (2004-09-01), IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways. J. Immunol., 2004-0173(5):3482-91.
PMID: 15322213
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Chang WL et al. (2004-08-01), Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality. J. Virol., 2004-078(16):8720-31.
PMID: 15280480
Sample Types: N/A

Phillips TM (2004-06-01), Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis. Electrophoresis, 2004-025(10):1652-9.
PMID: 15188254
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Sharif MN et al. (2004-05-15), IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis. J. Immunol., 2004-0172(10):6476-81.
PMID: 15128840
Sample Types: N/A
Applications: ELISA (Standard)

Copeland S et al. (2004-01-01), Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J. Immunol. Methods, 2004-0284(1):99-106.
PMID: 14736420
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Moreaux J et al. (2003-12-04), BAFF and APRIL protect myeloma cells from apoptosis induced by interleukin 6 deprivation and dexamethasone. Blood, 2003-1103(8):3148-57.
PMID: 15070697
Species: Human
Sample Types: Serum
Applications: SDS-Page

Uchida K et al. (2003-09-25), High-affinity autoantibodies specifically eliminate granulocyte-macrophage colony-stimulating factor activity in the lungs of patients with idiopathic pulmonary alveolar proteinosis. Blood, 2003-0103(3):1089-98.
PMID: 14512323
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Groh V et al. (2003-07-23), Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis. Proc. Natl. Acad. Sci. U.S.A., 2003-0100(16):9452-7.
PMID: 12878725
Sample Types: N/A
Applications: Control

Villalba S et al. (2003-07-22), Serum inactivation contributes to the failure of stromal-derived factor-1 to block HIV-I infection in vivo. J. Leukoc. Biol., 2003-074(5):880-8.
PMID: 12960279
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gasche C et al. (2003-06-01), Novel variants of the IL-10 receptor 1 affect inhibition of monocyte TNF-alpha production. J. Immunol., 2003-0170(11):5578-82.
PMID: 12759436
Species: Human
Sample Types: N/A, Whole Cells
Applications: Bioassay, ELISA (Standard)

Gee K et al. (2002-11-15), Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression. J. Immunol., 2002-1169(10):5660-72.
PMID: 12421945
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Liu X et al. (2002-05-01), Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells. Am. J. Physiol. Lung Cell Mol. Physiol., 2002-0282(5):L1049-56.
PMID: 11943670
Species: Primate - Papio anubis (Olive Baboon)
Sample Types: Whole Cells
Applications: Bioassay

Drouet M et al. (1999-01-01), The reduction of in vitro radiation-induced Fas-related apoptosis in CD34+ progenitor cells by SCF, FLT-3 ligand, TPO, and IL-3 in combination resulted in CD34+ cell proliferation and differentiation. Circulating NOD1 Activators and Hematopoietic NOD1 Contribute to Metabolic Inflammation and Insulin Resistance, 1999-017(5):273-85.
PMID: 10527462
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Fadok et al. (1998-02-15), Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF. J Clin Invest, 1998-0101(4):890-8.
PMID: 9466984

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Recombinant Human IL-10 Protein

Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 10/05/2020
Recombinant Human IL-10 Protein

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Application: In vitro bioactivity in cell culture

Verified Customer | Posted 01/10/2020
Recombinant Human IL-10 Protein

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Application: In vitro bioactivity in cell culture

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Verified Customer | Posted 10/08/2018
Recombinant Human IL-10 Protein

Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 09/20/2018

I used recombinant human IL-10 protein to polarize mouse macrophage to anti-inflammatory phenotype. Gene expression studies confirmed the significant increase in M2 genes and a decrease in the expression of M1 genes.
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Verified Customer | Posted 04/02/2018
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Recombinant Human IL-10 Protein

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