Human IL‑22 R alpha1 Antibody

Catalog # AF2770 | R&D Systems, Inc. a Bio-Techne Brand
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AF2770
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Key Product Details

Species Reactivity

Human

Applications

Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human IL‑22 R alpha1 Antibody

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human IL-22 R alpha1
Pro18-Thr228
Accession # Q8N6P7

Specificity

Detects human IL‑22 R alpha1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human IL-20 R beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IL‑22 R alpha1 Antibody

IL‑10 secretion Induced by IL‑22 and Neutralization by Human IL‑22 R alpha1 Antibody.

IL‑10 secretion Induced by IL‑22 and Neutralization by Human IL‑22 R alpha1 Antibody.

Recombinant Human IL-22 (Catalog # 782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcin-oma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-22 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2770). The ND50 is typically 0.2-1 µg/mL.

Applications for Human IL‑22 R alpha1 Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize IL‑22-induced IL‑10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. Marehalli, L. et al. (2004) Intl. Immunopharmacol. 4:679. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑22.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using AF2770 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-22 R alpha 1

IL-22 receptor, also known as IL-22 R alpha1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL-22 R alpha1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha1/IL-10 R beta is an IL-22 responsive receptor (2, 3), and IL-22 R alpha1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding

IL‑22 and preventing its association with IL-22 R alpha1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22% - 25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.

References

  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  2. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Boniface, K. et al. (2005) J. Immunol. 174:3695.
  4. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
  5. Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
  6. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
  7. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
  8. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099.
  9. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
  10. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
  11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
  12. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
  13. Wolk, K. et al. (2004) Immunity 21:241.
  14. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.

Long Name

Interleukin 22 Receptor

Alternate Names

CRF2-9, IL-22Ra1, IL-TIF-R1, IL22R alpha 1, IL22RA1

Entrez Gene IDs

58985 (Human); 230828 (Mouse)

Gene Symbol

IL22RA1

UniProt

Q8N6P7

Product Documents for Human IL‑22 R alpha1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL‑22 R alpha1 Antibody

For research use only

Citations for Human IL‑22 R alpha1 Antibody (5)

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  • Human IL-22 R alpha 1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 06/09/2020
    We used this antibody in an in-house ELISA along with mAb (2770) and protein standard (2770-LR) to quantify IL-22R alpha1 in human serum and plasma. This combination could not detect IL-22R alpha1 in our samples but generated a good standard curve.
    Human IL‑22 R alpha1 Antibody AF2770

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