Mouse CD4 APC-conjugated Antibody

Name: Mouse CD4 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB554A
Price: 152 EUR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB554A

Citations (2)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse CTL clone V4
Applications:	Flow Cytometry
Label:	Allophycocyanin (Excitation=620-650 nm, Emission=660-670 nm)
Source:	Monoclonal Rat IgG_2B Clone # GK1.5
Specificity:	Reacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells.

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Applications for Mouse CD4 APC-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse CD4 APC-conjugated Antibody

	Flow Cytometry Detection of CD4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) or (B) Rat IgG_2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

Item	Value
Entrez Gene IDs	920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names	CD4, CD4 antigen, CD4 antigen (p55), CD4 molecule, CD4 receptor, CD4mut, CD_antigen: CD4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Citations for Mouse CD4 APC-conjugated Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse CD4 APC-conjugated Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

A Alshamsan et al. (2020-01-01), Mitigation of Tacrolimus-Associated Nephrotoxicity by PLGA Nanoparticulate Delivery Following Multiple Dosing to Mice while Maintaining its Immunosuppressive Activity Sci Rep, 10(1):6675.
PMID: 32317681
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

R Niu et al. (2018-01-01), Ouabain Attenuates Sepsis-Induced Immunosuppression in Mice by Activation and Anti-Apoptosis of T Cells Med. Sci. Monit., 24(0):2720-2727.
PMID: 29717720

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Product Documents for Mouse CD4 APC-conjugated Antibody

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Mouse CD4 APC-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Mouse CD4 APC-conjugated Antibody

Scientific Data Examples for Mouse CD4 APC-conjugated Antibody

Flow Cytometry