Mouse IFN-gamma Antibody
Catalog # MAB485 | R&D Systems, Inc. a Bio-Techne Brand
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Product Summary for Mouse IFN-gamma Antibody
Immunogen
Specificity
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Host
Isotype
Scientific Data Images for Mouse IFN-gamma Antibody
Detection of IFN‑ gamma in Mouse Splenoctyes by Flow Cytometry.
Mouse splenocytes either (A) stimulated to induce Th1 cells or (B) unstimulated were stained with Rat Anti-Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB485) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on control antibody staining (Catalog # MAB006). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Detection of IFN‑ gamma in Mouse Splenoctyes by Flow Cytometry.
Mouse splenocytes either (A) stimulated for 4 hours with Cell Activation Cocktail 500x (5476) or (B) unstimulated were stained with Rat Anti-Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB485) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (FAB554A). Quadrant markers were set based on control antibody staining (MAB006). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma Antibody.
Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB485). The ND50 is typically 0.075-0.3 µg/mL.Applications for Mouse IFN-gamma Antibody
CyTOF-ready
Intracellular Staining by Flow Cytometry
Sample: Mouse splenocytes stimulated to induce Th1 cells or for 4 hours with Cell Activation Cocktail 500x (5476) were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Western Blot
Sample: Recombinant Mouse IFN-gamma (Catalog # 485-MI)
Neutralization
Formulation, Preparation and Storage
Purification
Endotoxin Level
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-gamma
Product Documents for Mouse IFN-gamma Antibody
Product Specific Notices for Mouse IFN-gamma Antibody
For research use only
Citations for Mouse IFN-gamma Antibody (22)
Citations are publications that use Bio-Techne products. Selected citations for Mouse IFN-gamma Antibody include:
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Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationJS Park et al. (2022-11-17), EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis Arthritis Research & Therapy, 2022-124(1):254.
PMID: 36397156 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationT Zhao et al. (2022-09-08), Lysosomal acid lipase, CSF1R and PD-L1 determine functions of CD11c+ myeloid-derived suppressor cells JCI Insight, 2022-00(0).
PMID: 35917184 -
Species: Mouse
Sample Types: In Vivo
Applications: NeutralizationRJ Napier et al. (2020-10-26), T cell-intrinsic role for Nod2 in protection against Th17-mediated uveitis Nat Commun, 2020-111(1):5406.
PMID: 33106495 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayJQ Yang et al. (2019-07-01), Ablation of RhoA impairs Th17 cell differentiation and alleviates house dust mite-triggered allergic airway inflammation J. Leukoc. Biol., 2019-00(0).
PMID: 31260596 -
Species: Mouse
Sample Types: Whole Cells
Applications: Functional AssayY Nam et al. (2018-06-07), Intraperitoneal infusion of mesenchymal stem cell attenuates severity of collagen antibody induced arthritis PLoS ONE, 2018-013(6):e0198740.
PMID: 29879214 -
Species: Mouse
Sample Types: Whole Cells
Applications: Functional AssayW Lei et al. (2018-01-18), Crucial role of OX40/OX40L signaling in a murine model of asthma Mol Med Rep, 2018-00(0).
PMID: 29344664 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationY Hu et al. (2017-02-07), Armc5 deletion causes developmental defects and compromises T-cell immune responses Nat Commun, 2017-08(0):13834.
PMID: 28169274 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayLee H et al. (2015-03-13), Mesenchymal stem/stromal cells protect against autoimmunity via CCL2-dependent recruitment of myeloid-derived suppressor cells. J Immunol, 2015-0194(8):3634-45.
PMID: 25769927 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationHe W et al. (2014-04-16), CD4(+) T cells from food allergy model are resistant to TCR-dependent apoptotic induction. Cytokine, 2014-068(1):32-9.
PMID: 24787054 -
Species: Mouse
Sample Types: Whole Cells
Applications: Functional AssayYoo et al. (2014-01-13), Transcriptional regulator CTR9 inhibits Th17 differentiation via repression of IL-17 expression. J Immunol, 2014-0192(4):1440-8.
PMID: 24420920 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationJin W et al. (2011-12-19), The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function. BMC Immunol., 2011-112(0):68.
PMID: 22182253 -
Species: Mouse
Sample Types: In Vivo
Applications: NeutralizationSarra M et al. (2011-03-25), IL-21 Promotes Skin Recruitment of CD4+ Cells and Drives IFN-{gamma}-Dependent Epidermal Hyperplasia. J. Immunol., 2011-0186(9):5435-42.
PMID: 21441456 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationJones GW et al. (2010-09-08), Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation. FASEB J., 2010-025(1):409-19.
PMID: 20826539 -
Species: Mouse
Sample Types: In Vivo
Applications: NeutralizationHorvat JC et al. (2010-03-12), Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease. J. Immunol., 2010-0184(8):4159-69.
PMID: 20228193 -
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA DevelopmentDeepak P et al. (2009-11-30), IL-13 from Th2-type cells suppresses induction of antigen-specific Th1 immunity in a T-cell lymphoma. Int. Immunol., 2009-122(1):53-63.
PMID: 19951958 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationMcKinley L et al. (2008-09-15), TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice. J. Immunol., 2008-0181(6):4089-97.
PMID: 18768865 -
Species: Mouse
Sample Types: In Vivo
Applications: NeutralizationMabalirajan U et al. (2008-09-01), Mitochondrial structural changes and dysfunction are associated with experimental allergic asthma. J. Immunol., 2008-0181(5):3540-8.
PMID: 18714027 -
Species: Mouse
Sample Types: In Vivo
Applications: NeutralizationIwanami K et al. (2008-03-01), Crucial role of the interleukin-6/interleukin-17 cytokine axis in the induction of arthritis by glucose-6-phosphate isomerase. Arthritis Rheum., 2008-058(3):754-63.
PMID: 18311788 -
Species: Mouse
Sample Types: In Vivo
Applications: NeutralizationNakamura M et al. (2008-02-02), Controlled delivery of T-box21 small interfering RNA ameliorates autoimmune alopecia (Alopecia Areata) in a C3H/HeJ mouse model. Am. J. Pathol., 2008-0172(3):650-8.
PMID: 18245811 -
Species: Mouse
Sample Types: In Vivo
Applications: NeutralizationSmith SE et al. (2007-10-03), Maternal immune activation alters fetal brain development through interleukin-6. J. Neurosci., 2007-127(40):10695-702.
PMID: 17913903 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCHaraguchi G et al. (2006-09-11), A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model. J. Mol. Cell. Cardiol., 2006-041(5):885-92.
PMID: 16963076 -
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization,Flow CytometryKamath AT et al. (2005-01-15), Dendritic cells and NK cells stimulate bystander T cell activation in response to TLR agonists through secretion of IFN-alpha beta and IFN-gamma. J. Immunol., 2005-0174(2):767-76.
PMID: 15634897
There are no citations that match your criteria.
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