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Mouse IL‑22 PE‑conjugated Antibody
Catalog # IC582P | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Mouse
Applications
Intracellular Staining by Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 140301
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Product Summary for Mouse IL‑22 PE‑conjugated Antibody
Immunogen
E. coli-derived recombinant mouse IL-22
Leu34-Val179
Accession # Q9JJY9
Leu34-Val179
Accession # Q9JJY9
Specificity
Detects mouse IL-22 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat IL-22 is observed, 15% cross-reactivity with recombinant human IL-22 is observed, and no cross-reactivity with recombinant mouse IL-10 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse IL‑22 PE‑conjugated Antibody
Detection of IL‑22 in Mouse Th17 Splenocytes by Flow Cytometry.
Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3e monoclonal antibody (MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (240-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 5 days were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (Catalog # FAB554A) and (A) Rat Anti-Mouse IL-22 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC582F) and (A) Rat Anti-Mouse IL-22 PE-conjugated Monoclonal Antibody (IC582P) or (B) isotype control antibody (IC006P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules.
Detection of IL‑22 in EL4 cells by Flow Cytometry.
EL4 cells were stained with Rat Anti-Mouse IL‑22 PE‑conjugated Monoclonal Antibody (Catalog # IC582P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Mouse IL‑22 PE‑conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Sample: Mouse splenocytes treated with plate-bound Rat anti-Mouse CD3 epsilon monoclonal antibody (Catalog # MAB484, 5 μg/ml) plus Goat anti-Mouse CD28 (Catalog # AF483, 5 μg/ml), Recombinant Human TGF-beta 1 (Catalog # 240-B, 10 ng/ml) Recombinant Mouse IL-23 (Catalog # 1887-ML, 20 ng/ml), Recombinant Mouse IL-6 (Catalog # 406-ML, 40 ng/ml), Recombinant Mouse IL-1 beta (Catalog # 401-ML, 10 ng/ml), and Rat anti-Mouse IFN-gamma (Catalog # MAB485, 10 μg/ml) for 5 days were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (Catalog # FC012) and EL‑4 mouse lymphoblast cell line.
Sample: Mouse splenocytes treated with plate-bound Rat anti-Mouse CD3 epsilon monoclonal antibody (Catalog # MAB484, 5 μg/ml) plus Goat anti-Mouse CD28 (Catalog # AF483, 5 μg/ml), Recombinant Human TGF-beta 1 (Catalog # 240-B, 10 ng/ml) Recombinant Mouse IL-23 (Catalog # 1887-ML, 20 ng/ml), Recombinant Mouse IL-6 (Catalog # 406-ML, 40 ng/ml), Recombinant Mouse IL-1 beta (Catalog # 401-ML, 10 ng/ml), and Rat anti-Mouse IFN-gamma (Catalog # MAB485, 10 μg/ml) for 5 days were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (Catalog # FC012) and EL‑4 mouse lymphoblast cell line.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-22
Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 146 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. The mouse IL-22 gene is localized to chromosome 10. Although it exists as a single copy gene in many mouse strains, the IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.
References
- Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
- Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
- Dumoutier, L. et al. (2000) PNAS 97:10144.
- Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
Long Name
Interleukin 22
Alternate Names
IL-TIF, IL22
Gene Symbol
IL22
UniProt
Product Documents for Mouse IL‑22 PE‑conjugated Antibody
Product Specific Notices for Mouse IL‑22 PE‑conjugated Antibody
For research use only
Citations for Mouse IL‑22 PE‑conjugated Antibody (6)
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Citations are publications that use Bio-Techne products. Selected citations for Mouse IL‑22 PE‑conjugated Antibody include:
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Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry(2016-09-21), During acute experimental infection with the reticulotropic Trypanosoma cruzi strain Tulahuen IL-22 is induced IL-23-dependently but is dispensable for protection Sci Rep, 2016-06(0):32927.
PMID: 27650379 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryWalker C et al. (2013-12-17), Intestinal intraepithelial lymphocyte-enterocyte crosstalk regulates production of bactericidal angiogenin 4 by Paneth cells upon microbial challenge. PLoS ONE, 2013-18(12):e84553.
PMID: 24358364 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryPandiyan P et al. (2012-09-19), The Role of IL-15 in Activating STAT5 and Fine-Tuning IL-17A Production in CD4 T Lymphocytes. J. Immunol., 2012-0189(9):4237-46.
PMID: 22993203 -
Species: Mouse
Sample Types: Whole Cells
Kumar P et al. (2012-06-27), IL-22 from conventional NK cells is epithelial regenerative and inflammation protective during influenza infection. Mucosal Immunol, 2012-06(1):69-82.
PMID: 22739232 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryNakajima K et al. (2011-02-23), Distinct Roles of IL-23 and IL-17 in the Development of Psoriasis-Like Lesions in a Mouse Model. J. Immunol., 2011-0186(7):4481-9.
PMID: 21346238 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryWahl C et al. (2009-04-15), IL-22-dependent attenuation of T cell-dependent (ConA) hepatitis in herpes virus entry mediator deficiency. J. Immunol., 2009-0182(8):4521-8.
PMID: 19342625
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