Mouse CD3 epsilon Antibody

Name: Mouse CD3 epsilon Antibody
Brand: R&D Systems
SKU: MAB484

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB484

Citations (14)

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Species Reactivity:	Mouse
Immunogen:	H-2K^b-specific mouse cytotoxic T-lymphocyte
Applications:	Cell Depletion, CyTOF-reported, Flow Cytometry, Functional Assay, Immunoprecipitation
Label:	Unconjugated
Source:	Monoclonal Hamster IgG Clone # 145-2C11
Specificity:	Detects mouse CD3 epsilon. Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.¹ Binds to the CD3 epsilon -chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.^3,4

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Applications for Mouse CD3 epsilon Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	Mouse splenocytes
Immunoprecipitation	Leo, O. et al. (1987) Proc. Natl. Acad. Sci. 84:1374.
Cell depletion	Small, M. et al. (1994) J. Immunol. Meth. 167:103.
CyTOF-reported	Fienberg, H.G. et al. (2012) Cytometry 81: 467. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
T Cell Stimulation	0.1-0.6 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse CD3 epsilon Antibody

	T Cell Stimulation Mouse CD3 epsilon Antibody Stimulates Cell Proliferation in Mouse T Cells. Hamster Anti-Mouse CD3e Monoclonal Antibody (Catalog # MAB484) stimulates cell proliferation in mouse T cells in a dose-dependent manner, as measured by Resazurin (Catalog # AR002). The ED₅₀ for this effect is typically 0.1-0.6 µg/mL.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CD3 epsilon

CD3 is composed of five different polypeptides ranging from 16‑28 kDa that are associated with the T cell receptor (TCR) complex and serve as its signal transducing element. The CD3/TCR complex is expressed on T cells and thymocytes. Binding of immobilized anti-CD3 can cause T cell activation that leads to any of several consequences, depending on the conditions. Activation by anti-CD3 in the presence of IL 2 has been reported to induce cell death, apparently via apoptosis.⁴ The antibody can be used to induce cytolytic activity against non-specific targets and also to block TCR-mediated cytolytic killing.¹145-2C11 has been used in a variety of studies concerned with allograft rejection and graft-vs host reaction in mice.^{6, 7}

Item	Value
References	Leo, O. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1374. Portoles, P. et al. (1989) J. Immunol. 142:4169. Coulie, P.G. et al. (1991) Eur. J. Immunol. 21:1703. Ucker, D.S. J. Meyers and P.S. Obermiller. (1992) J. Immunol. 149:1583. Small, M. et al. (1994) J. Immunol. Meth. 167:103. Alegre, M.L. et al. (1991) J. Immunol. 146:1184. Hendrickson, M. et al. (1995) Transplantation 60:828.
Entrez Gene IDs	916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)
Alternate Names	CD3 epsilon, CD3-epsilon, CD3e, CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), FLJ18683, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE

Citations for Mouse CD3 epsilon Antibody (14)

Showing 1 - 10 of 14 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse CD3 epsilon Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

SJ Sidles et al. (2019), High-Fat Diet Alters Immunogenic Properties of Circulating and Adipose Tissue-Associated Myeloid-Derived CD45+DDR2+ Cells Mediators Inflamm., 2019(0):1648614.
PMID: 31015794
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay

T Savinko et al. (2018), Filamin A Is Required for Optimal T Cell Integrin-Mediated Force Transmission, Flow Adhesion, and T Cell Trafficking J. Immunol., 0(0).
PMID: 29581355
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

E Engelowski et al. (2018), IL-23R Signaling Plays No Role in Myocardial Infarction Sci Rep, 8(1):17078.
PMID: 30459442
H Kinosada et al. (2017), HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors PLoS Pathog, 13(1):e1006120.
PMID: 28046066
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay

H Chen et al. (2017), mTORC2 controls Th9 polarization and allergic airway inflammation Allergy, 0(0).
PMID: 28273354
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

LT McDonald et al. (2017), Role of a novel immune modulating DDR2-expressing population in silica-induced pulmonary fibrosis PLoS ONE, 12(7):e0180724.
PMID: 28700752
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay

Gupta S et al. (2012), Developmental heterogeneity in DNA packaging patterns influences T-cell activation and transmigration. PLoS ONE, 7(9):e43718.
PMID: 22957031
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Jones GW et al. (2011), Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation. FASEB J., 25(1):409-19.
PMID: 20826539
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Jones GW et al. (2010), Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells. J. Immunol., 184(4):2130-9.
PMID: 20083667
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay

Sinclair LV et al. (2008), Phosphatidylinositol-3-OH kinase and nutrient-sensing mTOR pathways control T lymphocyte trafficking. Nat. Immunol., 9(5):513-21.
PMID: 18391955
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay

Li W et al. (2007), Protection of CD8+ T cells from activation-induced cell death by IL-18. J. Leukoc. Biol., 82(1):142-51.
PMID: 17400610
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay

Bheeshmachar G et al. (2006), Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cells. J. Immunol., 177(8):5041-50.
PMID: 17015687
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay

Ogilvie RL et al. (2005), Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay. J. Immunol., 174(2):953-61.
PMID: 15634918
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay

Kipnis J et al. (2004), Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration. J. Neurosci., 24(27):6133-43.
PMID: 15240805

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Mouse CD3 epsilon Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Background: CD3 epsilon

Citations for Mouse CD3 epsilon Antibody (14)

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