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Key Product Details

Species Reactivity

Mouse

Applications

Cell depletion, CyTOF-reported, Flow Cytometry, Immunoprecipitation, T Cell Stimulation

Label

Unconjugated

Antibody Source

Monoclonal Hamster IgG Clone # 145-2C11

Product Summary for Mouse CD3 epsilon Antibody

Immunogen

H-2Kb-specific mouse cytotoxic T-lymphocyte

Specificity

Detects mouse CD3 epsilon. Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.1 Binds to the CD3 epsilon-chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.3,4

Clonality

Monoclonal

Host

Hamster

Isotype

IgG

Scientific Data Images for Mouse CD3 epsilon Antibody

Mouse CD3e Antibody Stimulates Cell Proliferation antibody in Mouse T Cells.

Mouse CD3 epsilon Antibody Stimulates Cell Proliferation in Mouse T Cells.

Hamster Anti-Mouse CD3e Monoclonal Antibody (Catalog # MAB484) stimulates cell proliferation in mouse T cells in a dose-dependent manner, as measured by Resazurin (Catalog # AR002). The ED50 for this effect is typically 0.1-0.6 µg/mL.

Applications for Mouse CD3 epsilon Antibody

Application
Recommended Usage

Cell depletion

Small, M. et al. (1994) J. Immunol. Meth. 167:103.

CyTOF-reported

Fienberg, H.G. et al. (2012) Cytometry 81: 467. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes

Immunoprecipitation

Leo, O. et al. (1987) Proc. Natl. Acad. Sci. 84:1374.

T Cell Stimulation

0.1-0.6 µg/mL
Sample: Mouse splenic T cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 18 publications using MAB484 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD3 epsilon

CD3 is composed of five different polypeptides ranging from 16‑28 kDa that are associated with the T cell receptor (TCR) complex and serve as its signal transducing element. The CD3/TCR complex is expressed on T cells and thymocytes. Binding of immobilized anti-CD3 can cause T cell activation that leads to any of several consequences, depending on the conditions. Activation by anti-CD3 in the presence of IL 2 has been reported to induce cell death, apparently via apoptosis.4 The antibody can be used to induce cytolytic activity against non-specific targets and also to block TCR-mediated cytolytic killing.1 145-2C11 has been used in a variety of studies concerned with allograft rejection and graft-vs host reaction in mice.6, 7

References

  1. Leo, O. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1374.
  2. Portoles, P. et al. (1989) J. Immunol. 142:4169.
  3. Coulie, P.G. et al. (1991) Eur. J. Immunol. 21:1703.
  4. Ucker, D.S. J. Meyers and P.S. Obermiller. (1992) J. Immunol. 149:1583.
  5. Small, M. et al. (1994) J. Immunol. Meth. 167:103.
  6. Alegre, M.L. et al. (1991) J. Immunol. 146:1184.
  7. Hendrickson, M. et al. (1995) Transplantation 60:828.

Alternate Names

CD3e

Entrez Gene IDs

916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for Mouse CD3 epsilon Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CD3 epsilon Antibody

For research use only

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