Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunoprecipitation, Cell depletion, T Cell Stimulation, CyTOF-reported

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Recombinant Monoclonal Hamster IgG Clone # 145-2C11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD3 epsilon Antibodies »

Product Specifications

Immunogen

H-2Kb-specific mouse cytotoxic T-lymphocyte

Specificity

Detects mouse CD3 epsilon. Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.

Clonality

Monoclonal

Host

Hamster

Isotype

IgG

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Scientific Data Images for CD3 epsilon Antibody (145-2C11) [Allophycocyanin]

CD3 epsilon Antibody (145-2C11) [Allophycocyanin]

CD3 epsilon Antibody (145-2C11) [Allophycocyanin] [FAB484A] -

CD3 epsilon Antibody (145-2C11) [Allophycocyanin] [FAB484A] - Vial of APC conjugated antibody. APC is optimally excited at 650 nm by the Red laser (633 or 640 nm) and has an emission maximum of 660 nm.

Applications for CD3 epsilon Antibody (145-2C11) [Allophycocyanin]

Application
Recommended Usage

Cell depletion

Optimal dilutions of this antibody should be experimentally determined.

CyTOF-reported

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

T Cell Stimulation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product MAB484. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD3 epsilon

CD3 epsilon is a subunit of CD3. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Alternate Names

CD3e

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody (145-2C11) [Allophycocyanin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD3 epsilon Antibody (145-2C11) [Allophycocyanin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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