Proteome Profiler Mouse Apoptosis Array

Name: Proteome Profiler Mouse Apoptosis Array
Brand: R&D Systems
SKU: ARY031

R&D Systems, Inc. a Bio-Techne Brand

Catalog # ARY031

Citations (4)

Product Datasheet / COA / SDS

Contains 8 membranes - each spotted in duplicate with 21 different apoptosis antibodies

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Setting the standard in quality research reagents

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Citations (4) / Reviews

Summary for Proteome Profiler Mouse Apoptosis Array

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of mouse apoptosis-related proteins. Validated for analyte detection in cell lysates.

Troubleshooting Guide

Key Benefits

Detects 21 mouse apoptosis-related proteins simultaneously
Requires no specialized equipment
Compatible with LI-COR and chemiluminescence detection

Principle of the Assay

The Proteome Profiler Mouse Apoptosis Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of the bound analyte.

Why Use an Antibody Array to Detect Apoptosis-Related Proteins?

Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.

Kit Contents

Rectangular 8-Well Multi-dish
8 Mouse Apoptosis Array nitrocellulose membranes spotted with 21 different antibodies to mouse apoptosis-related proteins
Array Buffer 1
Array Buffer 8
Lysis Buffer 17
Chemi Reagent 1
Chemi Reagent 2
Detection Antibody Cocktail, Mouse Apoptosis Array
Streptavidin-HRP
Transparency Overlay Template
Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

Simultaneously detect the levels of these apoptosis-related proteins in a single sample.
Bad	Fas/TNFRSF6/CD95	MCL-1
Bcl-2	HIF-1α	p27/Kip1
Bcl-x	HO-1/HMOX1/HSP32	p53
Cleaved Caspase-3	HO-2/HMOX2	SMAC/Diablo
Catalase	HSP27	TNF RI/TNFRSF1A
Claspin	HSP60	TRAIL R2/TNFRSF10B
Cytochrome c	HSP70/HSPA1A	XIAP

Western blotting antibodies for analytes related to the Mouse Apoptosis Array Kit

Analyte	Affinity Purified Polyclonal Antibodies (Total)	Monoclonal Antibodies (Total)	Labeled Antibodies (Total)
Bad	AF819	MAB850
Bcl-2	AF813	MAB8272
		MAB810
Bcl-x	AF800
Cleaved Caspase-3		MAB835
Catalase	AF3398
Cytochrome c		MAB897
		MAB899
Fas/TNFRSF6/CD95	AF435		BAF435
HIF-1α	AF1935	MAB1536
HO-1/HMOX1/HSP32	AF3776	MAB3776
	AF3169
HO-2/HMOX2	AF3170	MAB3170
HSP27	AF1580
	AF15801
HSP60	AF1800	MAB1800
HSP70/HSPA1A	AF1663	MAB1663
p27/Kip1	AF2256	MAB2256
p53	AF1355	MAB1355	BAF1355
			HAF1355
TNF RI/TNFRSF1A	AF-425-PB	MAB430	BAF425
		MAB425
TRAIL R2/TNFRSF10B	AF721	MAB1540	BAF721
XIAP	AF8221	MAB822	BAF8221

R&D Systems' Apoptosis Array Kit is a rapid, sensitive, and economical tool to simultaneously detect the relative levels of expression of many apoptosis-related proteins in a single sample. No specialized equipment is necessary.

Product Specifications for Proteome Profiler Mouse Apoptosis Array

Species

Mouse

Scientific Data Examples for Proteome Profiler Mouse Apoptosis Array

	Expression of Mouse Apoptosis-related Proteins in Cell Lysate. Figure 1A: A20 mouse rectal carcinoma cell lysate (650 μg). Figure 1B: SW/3T3 Swiss albino mouse embryo fi broblast cell lysate (300 μg). Figure 1C: Ba/F3 mouse lymphocyte cell lysate (400 μg). Figure 1D: Hepa 1-6 mouse liver epithelial cell lysate (650 μg).
	Expression of Mouse Apoptosis-related Proteins in Cell Lysate. Figure 2: Lysates from C2C12 mouse muscle myoblast cells were left untreated or treated with 1 μM STS for 3 hours.

Preparation & Storage

Shipping Conditions	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Proteome Profiler Mouse Apoptosis Array (4)

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Citations are publications that use Bio-Techne products. Selected citations for Proteome Profiler Mouse Apoptosis Array include:

Species: Rat
Sample Types: Cell Lysates

MF Pittenger et al. (2022), MSC Pretreatment for Improved Transplantation Viability Results in Improved Ventricular Function in Infarcted Hearts International Journal of Molecular Sciences, 23(2).
PMID: 35054878
Species: Mouse
Sample Types: Tissue Homogenates

H Ishikawa et al. (2022), IkappaB? regulates the development of nonalcoholic fatty liver disease through the attenuation of hepatic steatosis in mice Scientific Reports, 12(1):11634.
PMID: 35804007
Species: Mouse
Sample Types: Cell Lysates

C Wallner et al. (2021), Deficiency of myostatin protects skeletal muscle cells from ischemia reperfusion injury Scientific Reports, 11(1):12572.
PMID: 34131275
Species: Mouse
Sample Types: Tissue Homogenates

Z Bognar et al. (2019), The effect of light exposure on the cleavage rate and implantation capacity of preimplantation murine embryos J. Reprod. Immunol., 132(0):21-28.
PMID: 30852462

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