TRAIL R2/TNFRSF10B was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Rat Anti-Mouse TRAIL R2/TNFRSF10B Monoclonal Antibody (Catalog # MAB1540) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of Purkinje neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse TRAIL R2/TNFRSF10B Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (cerebellum)

Western Blot

1 µg/mL
Sample: Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera (Catalog # 721-DR)

Reviewed Applications

Read 1 review rated 5 using MAB1540 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAIL R2/TNFRSF10B

Mouse TRAIL R2, also called DR5, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). Mouse TRAIL R2 cDNA encodes a 381 amino acid (aa) precursor protein containing an extracellular cysteine-rich domain, a transmembrane domain and a cytoplasmic death domain. Human and mouse TRAIL R2 share 49% aa sequence similarity. The death domains of human TRAIL R1 and TRAIL R2 share high homology with the death domain of mouse TRAIL R2, 76% and 79%, respectively. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. Besides the death domain containing receptors TRAIL R2 and TRAIL R1/DR4, three TRAIL decoy receptors, TRAIL R3/DcR1, TRAIL R4/DcR2, and OPG, have been reported.

References

Chaudhary, P.M. et al. (1997) Immunity 7:821.
Walczak, H. et al. (1997) EMBO J. 16:5386.
Golstein, P. (1997) Curr. Biol. 7:R750.
Wu, G.S. et al. (1999) Cancer Research 59:2770.

Long Name

TRAIL Receptor 2

Alternate Names

CD262, DR5, TNFRSF10B, TRAILR2

Entrez Gene IDs

8795 (Human); 21933 (Mouse)

Gene Symbol

TNFRSF10B

UniProt

Q9QZM4

Additional TRAIL R2/TNFRSF10B Products

Product Documents for Mouse TRAIL R2/TNFRSF10B Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse TRAIL R2/TNFRSF10B Antibody

For research use only

Citations
Reviews

Mouse TRAIL R2/TNFRSF10B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1540

Key Product Details

Species Reactivity

Validated:

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Applications

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Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse TRAIL R2/TNFRSF10B Antibody

TRAIL R2/TNFRSF10B in Mouse Brain.