Human/Mouse Bcl-x Antibody

Name: Human/Mouse Bcl-x Antibody
Brand: R&D Systems
SKU: AF800

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF800

Citations (8)

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Species Reactivity:	Human, Mouse
Immunogen:	KLH-coupled Bcl-x synthetic peptide CSAINGNPSWHLADSPAVNGA
Applications:	Immunoprecipitation, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Rabbit IgG
Specificity:	Detects human and mouse Bcl‑x in Western blots.

View Full Technical Details for Human/Mouse Bcl-x Antibody

Applications for Human/Mouse Bcl-x Antibody

	Recommended Concentration	Sample
Western Blot	0.3 µg/mL	See below
Simple Western	3 µg/mL	See below
Immunoprecipitation	5 µg/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse Bcl-x Antibody

	Western Blot Detection of Human and Mouse Bcl‑x by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line (5 x 10⁴cells, lane 1 and 2.5 x 10⁴cells, lane 2) and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bcl-x at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
	Immunoprecipitation Immunoprecipitation of Human Bcl-x. Bcl-x was immunoprecipitated from lysates (7 x 10⁵cells) of A431 human epithelial carcinoma cell line following incubation with 3 µg Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) for 30‑60 minutes at 4 °C. Bcl-x-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno-precipitated Bcl-x was detected by Western blot using 0.3 µg/mL Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). View our recommended buffer recipes for immunoprecipitation.
	Simple Western Detection of Human and Mouse Bcl‑x by Simple Western^TM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑x at approximately 34 kDa (as indicated) using 3 µg/mL of Rabbit Anti-Human/Mouse Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Bcl-x

The peptide corresponds to amino acids 49-68 of human and mouse, long and short forms of Bcl-x. The sequence used for immunization differs significantly from the sequences of the corresponding region of other members of the Bcl-2 family. Cysteine was added to the amino-terminal for coupling to KLH and for coupling to an affinity matrix.

Item	Value
Long Name	B Cell Lymphoma/Leukemia x
Entrez Gene IDs	598 (Human); 12048 (Mouse); 24888 (Rat)
Alternate Names	BCL2L1, Bcl-x, Bclx

Citations for Human/Mouse Bcl-x Antibody (8)

Showing 1 - 8 of 8 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse Bcl-x Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

M Kimsa-Dude et al. (2022), The Apoptotic Effect of Caffeic or Chlorogenic Acid on the C32 Cells That Have Simultaneously Been Exposed to a Static Magnetic Field International Journal of Molecular Sciences, 23(7).
PMID: 35409218
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

C Bao et al. (2021), Carbon ion triggered immunogenic necroptosis of nasopharyngeal carcinoma cells involving necroptotic inhibitor BCL-x Journal of Cancer, 12(5):1520-1530.
PMID: 33531997
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation

Schmitt E et al. (2007), Nuclear colocalization and interaction between bcl-xL and cdk1(cdc2) during G2/M cell-cycle checkpoint. Oncogene, 26(40):5851-65.
PMID: 17369848
Species: Human
Sample Types: Whole Cells
Applications: Western Blot

Armstrong T et al. (2004), Type I collagen promotes the malignant phenotype of pancreatic ductal adenocarcinoma. Clin. Cancer Res., 10(21):7427-37.
PMID: 15534120
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Mullen P et al. (2004), Antisense oligonucleotide targeting of Raf-1: importance of raf-1 mRNA expression levels and raf-1-dependent signaling in determining growth response in ovarian cancer. Clin. Cancer Res., 10(6):2100-8.
PMID: 15041731
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Sade H et al. (2004), The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells. J. Biol. Chem., 279(4):2937-44.
PMID: 14583609
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Raman VS et al. (2003), Ligation of CD27 on B cells in vivo during primary immunization enhances commitment to memory B cell responses. J. Immunol., 171(11):5876-81.
PMID: 14634097
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Ye Q et al. (2002), T cell costimulation through CD28 depends on induction of the Bcl-xgamma isoform: analysis of Bcl-xgamma-deficient mice. J. Exp. Med., 196(1):87-95.
PMID: 12093873

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Showing 1 - 8 of 8 citations Showing All

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Human/Mouse Bcl-x Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human/Mouse Bcl-x Antibody

Scientific Data Examples for Human/Mouse Bcl-x Antibody

Western Blot

Immunoprecipitation

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Bcl-x

Citations for Human/Mouse Bcl-x Antibody (8)

Product Documents for Human/Mouse Bcl-x Antibody

Product Datasheets

COA

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SDS

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