Detects mouse TNF RI in ELISAs and Western blots. In ELISAs, no cross-reactivity was observed with recombinant human (rh) TNF RI, rhTNF RII, recombinant mouse TNF RII, rhTNF-alpha, recombinant rat TNF-alpha, recombinant porcine TNF-alpha, or rhTNF-beta.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Scientific Data Images for Mouse/Rat TNF RI/TNFRSF1A Antibody

Detection of Mouse and Rat TNF RI/TNFRSF1A by Western Blot.

Western blot shows lysates of C6 rat glioma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB425) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Mouse TNF RI / TNFRSF1A ELISA Standard Curve

Recombinant Mouse TNF RI/TNFRSF1A (Catalog # 425-R1) was serially diluted and captured by Rat Anti-Mouse/Rat TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB425) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-425-PB) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse/Rat TNF RI/TNFRSF1A Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: C6 rat glioma cell line and NIH‑3T3 mouse embryonic fibroblast cell line

Mouse TNF RI/TNFRSF1A Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse TNF RI/TNFRSF1A Biotinylated Antibody (Catalog # BAF425)
Standard: Recombinant Mouse sTNF RI/TNFRSF1A Protein (Catalog # 425-R1)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RI/TNFRSF1A

TNF RI functions as a receptor for TNF-alpha and TNF-beta. It is a member of the TNF receptor superfamily and is designated TNFRSF1A.

Long Name

Tumor Necrosis Factor Receptor I

Alternate Names

CD120a, TNFRI, TNFRSF1A

Entrez Gene IDs

7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)

Gene Symbol

TNFRSF1A

UniProt

P25118

Additional TNF RI/TNFRSF1A Products

Product Documents for Mouse/Rat TNF RI/TNFRSF1A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse/Rat TNF RI/TNFRSF1A Antibody

For research use only

Citations
Reviews

Mouse/Rat TNF RI/TNFRSF1A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB425

Key Product Details

Species Reactivity

Validated:

Cited:

Applications