Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, DA3 mouse myeloma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat HO-2/HMOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3170) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HO-2/HMOX2 at approximately 38 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Detection of Human, Mouse, and Rat HO‑2/HMOX2 by Simple Western^TM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, DA3 mouse myeloma cell line, and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HO-2/HMOX2 at approximately 43 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HO-2/HMOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3170) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse HO‑2/HMOX2 by Simple Western^TM.

Simple Western lane view shows lysates of DA3 mouse myeloma cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HO-2/HMOX2 at approximately 43 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HO-2/HMOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3170) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat HO-2/HMOX2 Antibody

Application

Recommended Usage

Simple Western

5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, PC‑12 rat adrenal pheochromocytoma cell line, DA3 mouse myeloma cell line, and C2C12 mouse myoblast cell line

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, DA3 mouse myeloma cell line, and PC-12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HO-2/HMOX2

Heme Oxygenase 2 (HO-2), also known as HMOX2, is a 36 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, the constitutively expressed heme oxygenase-2 (HO-2/HMOX2) and the inducible heme oxygenase-1 (HO-1/HMOX1). HO-1 expression is induced by heme and other non-heme compounds. Human HO-2 shares 42% amino acid sequence identity with human HO-1 and 89% amino acid sequence identity with mouse and rat HO-2.

Long Name

Heme Oxygenase 2

Alternate Names

HMOX2, HO2

Entrez Gene IDs

3163 (Human); 15369 (Mouse); 79239 (Rat)

Gene Symbol

HMOX2

UniProt

P30519

Additional HO-2/HMOX2 Products

Product Documents for Human/Mouse/Rat HO-2/HMOX2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat HO-2/HMOX2 Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat HO-2/HMOX2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3170

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all HO-2/HMOX2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat HO-2/HMOX2 Antibody

Detection of Human/Mouse/Rat HO-2/HMOX2 by Western Blot.

Detection of Human, Mouse, and Rat HO‑2/HMOX2 by Simple WesternTM.

Detection of Mouse HO‑2/HMOX2 by Simple WesternTM.