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Mouse TRAIL R2/TNFRSF10B Biotinylated Antibody
Catalog # BAF721 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Summary for Mouse TRAIL R2/TNFRSF10B Biotinylated Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TRAIL R2/TNFRSF10B
Met1-Ser177
Accession # Q6GSD9
Met1-Ser177
Accession # Q6GSD9
Specificity
Detects TRAIL R2/TNFRSF10B in Western blots. In Western blots, no cross-reactivity with human (h) TRAIL R1, hTRAIL R2, hTRAIL R3, and hTRAIL R4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse TRAIL R2/TNFRSF10B Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: EL‑4 mouse lymphoblast cell line
Sample: EL‑4 mouse lymphoblast cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera (Catalog # 721-DR)
Sample: Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera (Catalog # 721-DR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Published Applications
Read 1 publication using BAF721 in the following applications:
Formulation, Preparation and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAIL R2/TNFRSF10B
Mouse TRAIL R2, also called DR5, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). Mouse TRAIL R2 cDNA encodes a 381 amino acid residue precursor protein containing an extracellular cysteine-rich domain, a transmembrane domain and a cytoplasmic death domain. Human and mouse TRAIL R2 share 49% amino acid sequence similarity. The death domains of human TRAIL R1 and TRAIL R2 share high homology with the death domain of mouse TRAIL R2, 76% and 79%, respectively. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The mouse TRAIL R2/Fc chimera neutralizes the ability of rhTRAIL to induce apoptosis. Besides the death domain containing receptors TRAIL R2 and TRAIL R1/DR4, three TRAIL decoy receptors, TRAIL R3/DcR1, TRAIL R4/DcR2, and OPG, have been reported.
References
- Chaudhary, P.M. et al. (1997) Immunity 7:821.
- Walczak, H. et al. (1997) EMBO J. 16:5386.
- Golstein, P. (1997) Curr. Biol. 7:R750.
- Wu, G.S. et al. (1999) Cancer Research 59:2770.
Long Name
TRAIL Receptor 2
Alternate Names
CD262, DR5, TNFRSF10B, TRAILR2
Gene Symbol
TNFRSF10B
UniProt
Product Documents for Mouse TRAIL R2/TNFRSF10B Biotinylated Antibody
Product Specific Notices for Mouse TRAIL R2/TNFRSF10B Biotinylated Antibody
For research use only
Citations for Mouse TRAIL R2/TNFRSF10B Biotinylated Antibody (1)
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Citations are publications that use Bio-Techne products. Selected citations for Mouse TRAIL R2/TNFRSF10B Biotinylated Antibody include:
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Species: Mouse
Sample Types: Whole Cells
Applications: ELISpot DevelopmentKhader SA et al. (2005-07-15), IL-23 compensates for the absence of IL-12p70 and is essential for the IL-17 response during tuberculosis but is dispensable for protection and antigen-specific IFN-gamma responses if IL-12p70 is available. J. Immunol., 2005-0175(2):788-95.
PMID: 16002675
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