Mouse TRAIL R2/TNFRSF10B Antibody

Name: Mouse TRAIL R2/TNFRSF10B Antibody
Brand: R&D Systems
SKU: AF721

Catalog # AF721 | R&D Systems, Inc. a Bio-Techne Brand

Citations (3)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse TRAIL R2/TNFRSF10B Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TRAIL R2/TNFRSF10B
Asn53-Ser177
Accession # Q6GSD9

Specificity

Detects mouse TRAIL R2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) TRAIL R2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse TRAIL R2/TNFRSF10B Antibody

Cytotoxicity Induced by TRAIL/TNFSF10 and Neutralization by Mouse TRAIL R2/TNFRSF10B Antibody.

In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, cytotoxicity elicited by Recombinant Human TRAIL/TNFSF10 (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRAIL R2/TNFRSF10B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF721). The ND₅₀ is typically 0.4-1.2 µg/mL.

Applications for Mouse TRAIL R2/TNFRSF10B Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: A20 mouse cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse TRAIL R2/TNFRSF10B

Neutralization

Measured by its ability to neutralize TRAIL/TNFSF10-induced cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.4-1.2 µg/mL in the presence of 12 ng/mL Recombinant Human TRAIL/TNFSF10, a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody, and 1 µg/mL actinomycin D.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 3 publications using AF721 in the following applications:

Flow Cytometry (3 Publications)

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAIL R2/TNFRSF10B

Mouse TRAIL R2, also called DR5, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). Mouse TRAIL R2 cDNA encodes a 381 amino acid residue precursor protein containing an extracellular cysteine-rich domain, a transmembrane domain and a cytoplasmic death domain. Human and mouse TRAIL R2 share 49% amino acid sequence similarity. The death domains of human TRAIL R1 and TRAIL R2 share high homology with the death domain of mouse TRAIL R2, 76% and 79%, respectively. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The mouse TRAIL R2/Fc chimera neutralizes the ability of rhTRAIL to induce apoptosis. Besides the death domain containing receptors TRAIL R2 and TRAIL R1/DR4, three TRAIL decoy receptors, TRAIL R3/DcR1, TRAIL R4/DcR2, and OPG, have been reported.

References

Chaudhary, P.M. et al. (1997) Immunity 7:821.
Walczak, H. et al. (1997) EMBO J. 16:5386.
Golstein, P. (1997) Curr. Biol. 7:R750.
Wu, G.S. et al. (1999) Cancer Research 59:2770.

Long Name

TRAIL Receptor 2

Alternate Names

CD262, DR5, TNFRSF10B, TRAILR2

Entrez Gene IDs

8795 (Human); 21933 (Mouse)

Gene Symbol

TNFRSF10B

UniProt

Q6GSD9

Product Documents for Mouse TRAIL R2/TNFRSF10B Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse TRAIL R2/TNFRSF10B Antibody

For research use only

Citations for Mouse TRAIL R2/TNFRSF10B Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse TRAIL R2/TNFRSF10B Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

(2016-06-07), IAP antagonists sensitize murine osteosarcoma cells to killing by TNF? Oncotarget, 2016-07(23):33866-86.
PMID: 27129149
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Lundqvist A et al. (2009-02-06), Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells. Blood, 2009-0113(24):6120-7.
PMID: 19202127
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Park SJ et al. (2006-05-04), P-glycoprotein enhances TRAIL-triggered apoptosis in multidrug resistant cancer cells by interacting with the death receptor DR5. Biochem. Pharmacol., 2006-072(3):293-307.
PMID: 16753135

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