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His Tag Antibody Best Seller
Catalog # MAB050 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Multi-Species
Applications
CyTOF-ready, Flow Cytometry, Immunoaffinity Purification, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # AD1.1.10
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Product Summary for His Tag Antibody
Immunogen
His-tagged peptide
Specificity
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl‑terminus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for His Tag Antibody
Detection of His Tag by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged human BAI-1. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Proteins by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with His-tagged proteins was stained with Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
SARS-Cov-2 Spike 1 His tag protein binding to ACE-2-transfected Human Cell Line is detected by His tag Antibody.
Recombinant SARS-Cov-2 Spike 1 His-tagged protein (10522-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and was detected with Mouse Anti-His Allophycocyanin-conjugated Monoclonal Antibody (IC050A, filled histogram). No staining was observed in the absence of protein (open histogram). Staining was performed using our Staining Membrane-Associated Proteins protocol.Applications for His Tag Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with His-tagged proteins and SARS-Cov-2 Spike 1 His tag protein binding to ACE-2-transfected Human Cell Line
Sample: HEK293 human embryonic kidney cell line transfected with His-tagged proteins and SARS-Cov-2 Spike 1 His tag protein binding to ACE-2-transfected Human Cell Line
Immunoaffinity Purification
Immobilized anti-His antibody can be used to affinity purify proteins containing exposed His tags. The column capacity must be determined for each individual His-tagged protein.
Simple Western
10 µg/mL
Sample: CHO Chinese hamster ovary cell line transfected with His-tagged Human S1P4/EDG-6
Sample: CHO Chinese hamster ovary cell line transfected with His-tagged Human S1P4/EDG-6
Western Blot
0.2 µg/mL
Sample: HEK293 human embryonic kidney cell line and CHO Chinese hamster ovary cell line transfected with His-tagged proteins
Sample: HEK293 human embryonic kidney cell line and CHO Chinese hamster ovary cell line transfected with His-tagged proteins
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 11 reviews rated 4.9 using MAB050 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Endotoxin Level
<0.20 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: His Tag
Long Name
Histidine Tag
Alternate Names
polyHistidine
Product Documents for His Tag Antibody
Product Specific Notices for His Tag Antibody
For research use only
Citations for His Tag Antibody (57)
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Citations are publications that use Bio-Techne products. Selected citations for His Tag Antibody include:
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Species: Human
Sample Types: Cell Lysates
Applications: Western BlotC Wilkinson et al. (2022-06-02), Improved yield of recombinant human IFN-alpha2b from mammalian cells using heterologous signal peptide approach Protein Expression and Purification, 2022-0198(0):106125.
PMID: 35659600 -
Species: N/A
Sample Types: Recombinant Protein
Applications: Western BlotM Sakari et al. (2022-04-01), Crystal structures of pertussis toxin with NAD+ and analogs provide structural insights into the mechanism of its cytosolic ADP-ribosylation activity The Journal of Biological Chemistry, 2022-0298(5):101892.
PMID: 35378130 -
Species: N/A
Sample Types: Complex Sample Type
Applications: ELISA (detection)M Jiang et al. (2022-03-24), Structural dynamics of receptor recognition and pH-induced dissociation of full-length Clostridioides difficile Toxin B PloS Biology, 2022-020(3):e3001589.
PMID: 35324891 -
Species: Human
Sample Types: Cell Lysates
Applications: ImmunoprecipitationF Okumura et al. (2021-10-22), ZSWIM8 is a myogenic protein that partly prevents C2C12 differentiation Scientific Reports, 2021-111(1):20880.
PMID: 34686700 -
Species: Human
Sample Types: Recombinant Protein
Applications: Western BlotS Maneewatch et al. (2021-10-05), Evaluation of a genus-specific rGroEL1-524 IgM-ELISA and commercial ELISA kits during the course of leptospirosis in Thailand Scientific Reports, 2021-111(1):19785.
PMID: 34611273 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotN Kulminskay et al. (2021-09-17), Optimized expression and purification of adipose triglyceride lipase improved hydrolysis and transacylation catalytic activities in vitro The Journal of Biological Chemistry, 2021-00(0):101206.
PMID: 34543623 -
Species: Human
Sample Types: Whole Cells,Cell Lysates
Applications: ICC,Immunoprecipitation,Co-IP,Western BlotE Seidel et al. (2021-04-06), A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain Nature Communications, 2021-012(1):2061.
PMID: 33824318 -
Species: Yeast
Sample Types: Cell Lysates
Applications: Western BlotYC Li et al. (2021-01-15), Structure and noncanonical Cdk8 activation mechanism within an Argonaute-containing Mediator kinase module Science Advances, 2021-07(3).
PMID: 33523904 -
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation,Western BlotGT Banks et al. (2020-08-12), Forward genetics identifies a novel sleep mutant with sleep state inertia and REM sleep deficits Sci Adv, 2020-06(33):eabb3567.
PMID: 32851175 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotS Jaillard et al. (2020-06-20), New insights into the genetic basis of premature ovarian insufficiency: Novel causative variants and candidate genes revealed by genomic sequencing Maturitas, 2020-0141(0):9-19.
PMID: 33036707 -
Species: Human
Sample Types: Plasma
Applications: ELISA DevelopmentS Ols et al. (2020-03-24), Route of Vaccine Administration Alters Antigen Trafficking but Not Innate or Adaptive Immunity Cell Rep, 2020-030(12):3964-3971.e7.
PMID: 32209459 -
Species: Bacteria - Streptococcus suis
Sample Types: Recombinant Protein
Applications: Western BlotA Dumesnil et al. (2019-12-10), Enolase and dipeptidyl peptidase IV protein sub-unit vaccines are not protective against a lethal Streptococcus suis serotype 2 challenge in a mouse model of infection BMC Vet. Res., 2019-115(1):448.
PMID: 31823789 -
Species: Human
Sample Types: Whole Cells
Applications: Western BlotMER Maitland et al. (2019-07-08), The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation Sci Rep, 2019-09(1):9864.
PMID: 31285494 -
Species: Human
Sample Types: Cell Culture Supernates,Protein
Applications: Epitope Mapping,ELISA DevelopmentP Zhang et al. (2019-06-11), Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity Cell Rep, 2019-027(11):3117-3123.e5.
PMID: 31189099 -
Species: Human
Sample Types: Whole Cells
Applications: KnockdownM Yeste-Vela et al. (2019-05-01), The potential of brentuximab vedotin, alone or in combination with current clinical therapies, in the treatment of testicular germ cell tumors Am J Cancer Res, 2019-09(5):855-871.
PMID: 31218098 -
Species: Guinea Pig
Sample Types: Recombinant Protein
Applications: ELISA DevelopmentL Lei et al. (2019-04-09), The HIV-1 Envelope Glycoprotein C3/V4 Region Defines a Prevalent Neutralization Epitope following Immunization Cell Rep, 2019-027(2):586-598.e6.
PMID: 30970260 -
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Development (Capture)DM Heuberger et al. (2019-02-28), Thrombin cleaves and activates the protease-activated receptor 2 dependent on thrombomodulin co-receptor availability Thromb. Res., 2019-0177(0):91-101.
PMID: 30861432 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayL Herviou et al. (2019-02-12), EZH2 is overexpressed in transitional preplasmablasts and is involved in human plasma cell differentiation Leukemia, 2019-00(0).
PMID: 30755708 -
Sample Types: Recombinant Protein
Applications: ELISA (Capture)SM Chin et al. (2018-11-08), Structure of the 4-1BB/4-1BBL complex and distinct binding and functional properties of utomilumab and urelumab Nat Commun, 2018-19(1):4679.
PMID: 30410017 -
Sample Types: Recombinant Protein
Applications: Western BlotMK Kim et al. (2018-11-08), Reciprocal negative regulation between the tumor suppressor protein p53 and B cell CLL/lymphoma 6 (BCL6) via control of caspase-1 expression J. Biol. Chem., 2018-10(0).
PMID: 30409904 -
Species: Insect
Sample Types: Virus
Applications: Western BlotS Iyer et al. (2018-10-17), C9orf72, a protein associated with amyotrophic lateral sclerosis (ALS) is a guanine nucleotide exchange factor PeerJ, 2018-16(0):e5815.
PMID: 30356970 -
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPRB Åkerström et al. (2018-04-11), rA1M-035, a physicochemically improved human recombinant ?1-microglobulin, has therapeutic effects in rhabdomyolysis-induced acute kidney injury Antioxid. Redox Signal., 2018-00(0).
PMID: 29471681 -
Species: Human
Sample Types: Recombinant Protein
Applications: Cross-linkingF Arce Varga et al. (2018-03-22), Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies Cancer Cell, 2018-00(0).
PMID: 29576375 -
Sample Types: Cell Lysates
Applications: BioassayJH Urban et al. (2017-11-15), Phage display and selection of lanthipeptides on the carboxy-terminus of the gene-3 minor coat protein Nat Commun, 2017-18(1):1500.
PMID: 29138389 -
Applications: Binding AssayD Wensel et al. (2017-07-25), Discovery and Characterization of a Novel CD4-Binding Adnectin with Potent Anti-HIV Activity Antimicrob. Agents Chemother., 2017-00(0).
PMID: 28584151 -
Species: Primate
Sample Types: Plasma
Applications: ELISA Development (Capture)P Martinez-M et al. (2017-05-16), Particulate Array of Well-Ordered HIV Clade C Env Trimers Elicits Neutralizing Antibodies that Display a Unique V2 Cap Approach Immunity, 2017-046(5):804-817.e7.
PMID: 28514687 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayM Schoenhals et al. (2017-05-02), Hypoxia favors the generation of human plasma cells Cell Cycle, 2017-00(0):1-14.
PMID: 28463531 -
Species: Human
Sample Types: Protein
Applications: Western BlotU Sharma et al. (2017-03-10), Structural basis of homo- and heterotrimerization of collagen I Nat Commun, 2017-08(0):14671.
PMID: 28281531 -
Species: Human
Sample Types: Whole Cells
Applications: Cross-linking(2016-11-18), Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis Sci Rep, 2016-16(0):36862.
PMID: 27857173 -
Sample Types: Whole Cells
Applications: Flow CytometryAlexey A Lugovskoy (2016-11-17), Improving the developability of an anti-EphA2 single-chain variable fragment for nanoparticle targeting MAbs, 2016-10(0):0.
PMID: 27854147 -
Species: Guinea Pig
Sample Types: Serum
Applications: ELISA Development (Capture)(2016-08-03), Thermostability of Well-Ordered HIV Spikes Correlates with the Elicitation of Autologous Tier 2 Neutralizing Antibodies PLoS Pathog, 2016-012(8):e1005767.
PMID: 27487086 -
Species: Human
Sample Types: Recombinant Protein
Applications: Functional Assay(2016-05-23), A Novel Bispecific Antibody Targeting EGFR and cMet that is Effective Against EGFR Inhibitor- Resistant Lung Tumors Cancer Res, 2016-00(0).
PMID: 27216193 -
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot(2016-05-18), HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins J Immunol, 2016-00(0).
PMID: 27194785 -
Species: Human
Sample Types: Complex Sample Type
Applications: BioassayK Griffiths et al. (2016-04-01), I-bodies: human single domain antibodies that antagonize chemokine receptor CXCR4 J Biol Chem, 2016-00(0).
PMID: 27036939 -
Species: Hamster
Sample Types: Whole Cells
Applications: NeutralizationOskarsson M et al. (2015-04-28), Heparan Sulfate Proteoglycans Are Important for Islet Amyloid Formation and Islet Amyloid Polypeptide-induced Apoptosis. J Biol Chem, 2015-0290(24):15121-32.
PMID: 25922077 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotKonitsiotis A et al. (2014-12-12), Topological analysis of Hedgehog acyltransferase, a multipalmitoylated transmembrane protein. J Biol Chem, 2014-1290(6):3293-307.
PMID: 25505265 -
Sample Types: Chip
(2014-07-01), Generation of a high-fidelity antibody against nerve growth factor using library scanning mutagenesis and validation with structures of the initial and optimized Fab-antigen complexes. MAbs, 2014-06(4):1059-68.
PMID: 24830649 -
Species: Mouse
Sample Types: Whole Cells
Applications: Cross-linkingMadireddi S et al. (2014-06-23), Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies. J Exp Med, 2014-0211(7):1433-48.
PMID: 24958847 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotKondo et al. (2014-04-08), Memo has a novel role in S1P signaling and is [corrected] crucial for vascular development. PLoS ONE, 2014-09(4):e94114.
PMID: 24714781 -
Species: Mouse
Sample Types: Whole Cells
Applications: Western BlotLiu Q et al. (2014-03-13), Blockade of Fas signaling in breast cancer cells suppresses tumor growth and metastasis via disruption of Fas signaling-initiated cancer-related inflammation. J Biol Chem, 2014-0289(16):11522-35.
PMID: 24627480 -
Species: Human,Mouse
Sample Types: Whole Cells
Applications: Flow CytometryMendel I et al. (2014-01-01), VB-201, an oxidized phospholipid small molecule, inhibits CD14- and Toll-like receptor-2-dependent innate cell activation and constrains atherosclerosis. Clin Exp Immunol, 2014-0175(1):126-37.
PMID: 24116867 -
Species: Bovine
Sample Types: Recombinant Protein
Applications: Western BlotBai X et al. (2013-12-23), Novel septin 9 repeat motifs altered in neuralgic amyotrophy bind and bundle microtubules. J Cell Biol, 2013-1203(6):895-905.
PMID: 24344182 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotMatsui T et al. (2013-10-23), Pigment epithelium-derived factor (PEDF) binds to caveolin-1 and inhibits the pro-inflammatory effects of caveolin-1 in endothelial cells. Biochem Biophys Res Commun, 2013-1441(2):405-10.
PMID: 24161393 -
Species: Human
Sample Types: Whole Cells
Applications: Cross-linkingKyathanahalli et al. (2013-10-18), Cross-species withdrawal of MCL1 facilitates postpartum uterine involution in both the mouse and baboon. Endocrinology, 2013-1154(12):4873-84.
PMID: 24140717 -
Species: Mouse
Sample Types: Recombinant Proteins
Applications: Surface Plasmon Resonance (SPRFranco A et al. (2013-04-24), Human Fc receptor-like 5 binds intact IgG via mechanisms distinct from those of Fc receptors. J Immunol, 2013-0190(11):5739-46.
PMID: 23616577 -
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA DevelopmentRose et al. (2013-02-13), Mutation of Y407 in the CH3 domain dramatically alters glycosylation and structure of human IgG. MAbs, 2013-05(2):219-28.
PMID: 23406897 -
Species: Human
Sample Types: Recombinant Protein
Applications: BioassayRicciardi M et al. (2012-05-02), Comparison of epithelial differentiation and immune regulatory properties of mesenchymal stromal cells derived from human lung and bone marrow. PLoS ONE, 2012-07(5):e35639.
PMID: 22567106 -
Sample Types: Recombinant Protein
Applications: Cross-linkingSimhadri VL et al. (2012-01-01), A novel role for reciprocal CD30-CD30L signaling in the cross-talk between natural killer and dendritic cells. Biol. Chem., 2012-0393(1):101-6.
PMID: 22628304 -
Sample Types: Recombinant Protein
Applications: Cross-linkingStephenson-Famy A et al. (2011-12-22), Antiapoptotic signaling via MCL1 confers resistance to caspase-3-mediated apoptotic cell death in the pregnant human uterine myocyte. Mol. Endocrinol., 2011-126(2):320-30.
PMID: 22194343 -
Species: Mouse
Sample Types: Serum
Applications: ELISA DevelopmentChao C et al. (2011-07-25), C17 prevents inflammatory arthritis and associated joint destruction in mice. PLoS ONE, 2011-06(7):e22256.
PMID: 21799806 -
Species: Virus
Sample Types: Recombinant Protein
Applications: Western BlotWu ZL et al. (2009-01-06), Active 1918 pandemic flu viral neuraminidase has distinct N-glycan profile and is resistant to trypsin digestion. Biochem. Biophys. Res. Commun., 2009-0379(3):749-53.
PMID: 19133226 -
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA DevelopmentSengle G et al. (2008-07-02), A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7. J. Mol. Biol., 2008-0381(4):1025-39.
PMID: 18621057 -
Sample Types: Recombinant Protein
Applications: Affinity PurificationSengle G et al. (2008-03-13), Targeting of bone morphogenetic protein growth factor complexes to fibrillin. J. Biol. Chem., 2008-0283(20):13874-88.
PMID: 18339631 -
Sample Types: Recombinant Protein
Applications: Cross-linkingKovacic N et al. (2007-03-15), The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis. J. Immunol., 2007-0178(6):3379-89.
PMID: 17339432 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryFransson J et al. (2006-07-15), Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies. Int. J. Cancer, 2006-0119(2):349-58.
PMID: 16477633 -
Sample Types: Recombinant Protein
Applications: Western BlotCortesio CL et al. (2006-03-03), Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Arch. Biochem. Biophys., 2006-0449(1):164-70.
PMID: 16554018 -
Species: Mouse
Sample Types: Whole Cells
Applications: Binding AssayLee FH et al. (2004-06-08), Receptor-ligand binding in the cell-substrate contact zone: a quantitative analysis using CX3CR1 and CXCR1 chemokine receptors. Biochemistry, 2004-043(22):7179-86.
PMID: 15170355
There are no citations that match your criteria.
Customer Reviews for His Tag Antibody (11)
4.9 out of 5
11 customer
ratings
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Application: Western BlotSample Tested: 293T human embryonic kidney cell lineSpecies: HumanVerified Customer | Posted 04/06/2022
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Application: Western BlotSample Tested: HEK293T human embryonic kidney cell lineSpecies: HumanVerified Customer | Posted 10/29/2021
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Application: Western BlotSample Tested: Recombinant proteinSpecies: MouseVerified Customer | Posted 08/06/2021
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Application: Flow CytometrySample Tested: 7TD1 mouse hybridoma cell lineSpecies: MouseVerified Customer | Posted 05/07/2019
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Application: Cell depletionSample Tested: 4T1 mouse breast cancer cell lineSpecies: MouseVerified Customer | Posted 01/11/2019
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Application: Western BlotSample Tested: 293T human embryonic kidney cell lineSpecies: HumanVerified Customer | Posted 07/23/2018293T cells were transfected with pCDNA 3.1-Control or His-RIP1 for 48h. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1 um/ml Human His Tag Antibody (MAB050). A specific band was detected for His-RIP1 at approximately 75 kDa. This experiment was conducted under reducing conditions
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Application: Simple WesternSample Tested: Purified proteinSpecies: HumanVerified Customer | Posted 06/20/2018
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Application: Western BlotSample Tested: 293T human embryonic kidney cell lineSpecies: MouseVerified Customer | Posted 07/15/2017
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Application: Western BlotSample Tested: HEK293T human embryonic kidney cell lineSpecies: HumanVerified Customer | Posted 06/28/2016cell lysates transformed
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Application: Western BlotSample Tested: Recombinant proteinSpecies: HumanVerified Customer | Posted 06/28/2016
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Application: ELISASample Tested: Human recombinant test antibodySpecies: HumanVerified Customer | Posted 10/26/2015Specificity: Specific
Sensitivity: Sensitive
Buffer: TBS + Tween + BSA
Dilution: used at 2ug/mL
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FAQs for His Tag Antibody
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Q: Can the His Tag Antibody (MAB050) recognize all His-tagged proteins?
A: MAB050 has been validated specifically for the detection of His-tags localized to the amino- or carboxyl-terminus of a peptide. It is also expected to detect any series of 6 or more histidine residues as long as this epitope is conformationally accessible. For applications utilizing linearized proteins, it is likely that MAB050 will have access to any consecutive histidine regions. If used with protein in its native conformation, there is the potential that the protein will be folded such that the antibody is not able to bind its target.
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Q: This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?
A: The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.
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Q: What is the difference between His Tag Antibodies, Catalog #s MAB050 and MAB050R?
A: The primary difference is how the antibodies are produced. Catalog # MAB050R is made using recombinant DNA technology while Catalog # MAB050 is produced from a hybridoma.
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