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His Tag Antibody

Detection of His Tag antibody by Western Blot.
(4)
Detection of His Tag antibody in HEK293 Human Cell Line Transfected with His-tagged Proteins antibody by Flow Cytometry.
Detection of His Tag antibody by Simple WesternTM.
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MAB050-SP
MAB050-500
MAB050-100

Key Product Details

Immunogen: His-tagged peptide
Applications: Affinity Purification, CyTOF-ready, Flow Cytometry, Simple Western, Western Blot
Label: Unconjugated
Source: Monoclonal Mouse IgG1 Clone # AD1.1.10
Specificity:

Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl‑terminus.

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Citations (46)

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Customer Reviews (11)

4.8 out of 5
11 customer ratings
5 stars
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  • His Tag Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: HEK293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 10/29/2021
    His Tag Antibody Western Blot MAB050
  • His Tag Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: Recombinant protein
    Species: Mouse
    Verified Customer | Posted 08/06/2021
    His Tag Antibody Western Blot MAB050
  • His Tag Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: CTLL-2 mouse cytotoxic T cell line,HT-2 mouse T cell line
    Species: Mouse
    Verified Customer | Posted 05/14/2019
  • His Tag Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: 7TD1 mouse hybridoma cell line
    Species: Mouse
    Verified Customer | Posted 05/07/2019
  • His Tag Antibody
    Name: Anonymous
    Application: Cell Depl
    Sample Tested: 4T1 mouse breast cancer cell line
    Species: Mouse
    Verified Customer | Posted 01/11/2019
  • His Tag Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 07/23/2018
    293T cells were transfected with pCDNA 3.1-Control or His-RIP1 for 48h. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1 um/ml Human His Tag Antibody (MAB050). A specific band was detected for His-RIP1 at approximately 75 kDa. This experiment was conducted under reducing conditions
    His Tag Antibody Western Blot MAB050
  • His Tag Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Purified protein
    Species: Human
    Verified Customer | Posted 06/20/2018
    His Tag Antibody Simple Western MAB050
  • His Tag Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: 293T human embryonic kidney cell line
    Species: Mouse
    Verified Customer | Posted 07/15/2017
    His Tag Antibody Western Blot MAB050
  • His Tag Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: HEK293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 06/28/2016
    cell lysates transformed
  • His Tag Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 06/28/2016
    His Tag Antibody Western Blot MAB050
  • His Tag Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Human recombinant test antibody
    Species: Human
    Verified Customer | Posted 10/26/2015
    Specificity: Specific
    Sensitivity: Sensitive
    Buffer: TBS + Tween + BSA
    Dilution: used at 2ug/mL

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FAQs

  • This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

Product Documents

Certificate of Analysis

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