His Tag PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC050P
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human, Mouse
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry, Bioassay, CAR-T (Flow Cytometry)
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # AD1.1.10
Product Specifications
Immunogen
His-tagged peptide
Specificity
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl-terminus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for His Tag PE-conjugated Antibody
Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Protein by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with His-tagged protein were stained with Mouse Anti-His Tag PE-conjugated Monoclonal Antibody (Catalog # IC050P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.Applications for His Tag PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with His-tagged protein fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: HEK293 human embryonic kidney cell line transfected with His-tagged protein fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Reviewed Applications
Read 12 reviews rated 5 using IC050P in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: His Tag
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.
Long Name
Histidine Tag
Alternate Names
polyHistidine
Additional His Tag Products
Product Documents for His Tag PE-conjugated Antibody
Product Specific Notices for His Tag PE-conjugated Antibody
For research use only
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