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His Tag APC-conjugated Antibody
R&D Systems, Inc. a Bio-Techne Brand
Catalog # IC050A
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Key Product Details
| Immunogen: | His-tagged peptide |
| Applications: | Flow Cytometry |
| Label: | Allophycocyanin (Excitation=620-650 nm, Emission=660-670 nm) |
| Source: | Monoclonal Mouse IgG1 Clone # AD1.1.10 |
| Specificity: |
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl‑terminus. |
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Flow CytometrySARS-Cov-2 Spike 1 His tag protein binding to ACE-2-transfected Human Cell Line is detected by His tag Antibody. (A) Recombinant SARS-Cov-2 Spike 1 His-tagged protein (10522-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP and was detected with Mouse Anti-His Allophycocyanin-conjugated Monoclonal Antibody (Catalog # IC050A). (B) No staining was observed in the absence of protein. Staining was performed using our Staining Membrane-Associated Proteins protocol. |
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Intracellular Staining by Flow CytometryDetection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Protein by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with His-tagged protein was stained with Mouse Anti-His Tag APC-conjugated Monoclonal Antibody (Catalog # IC050A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
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Citations for His Tag APC-conjugated Antibody (3)
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Citations are publications that use Bio-Techne products. Selected citations for His Tag APC-conjugated Antibody include:
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Species: Xenograft
Sample Types: Whole Cells
Applications: Flow CytometryWN Liu et al. (2022), Successful targeting of PD-1/PD-L1 with chimeric antigen receptor-natural killer cells and nivolumab in a humanized mouse cancer model Science Advances, 8(47):eadd1187.
PMID: 36417514 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryA Cho et al. (2019), Single-Cell Analysis Suggests that Ongoing Affinity Maturation Drives the Emergence of Pemphigus Vulgaris Autoimmune Disease Cell Rep, 28(4):909-922.e6.
PMID: 31340153 -
Species: Human
Sample Types: Whole Cells
Applications: Binding AssayT Osada et al. (2018), Polyfunctional anti-human epidermal growth factor receptor 3 (anti-HER3) antibodies induced by HER3 vaccines have multiple mechanisms of antitumor activity against therapy resistant and triple negative breast cancers Breast Cancer Res., 20(1):90.
PMID: 30092835
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