His Tag APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC050A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human, Xenograft
Applications
Validated:
Flow Cytometry, Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry, Immunocytochemistry, Binding Assay
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # AD1.1.10
Product Specifications
Immunogen
His-tagged peptide
Specificity
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl‑terminus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for His Tag APC-conjugated Antibody
SARS-Cov-2 Spike 1 His tag protein binding to ACE-2-transfected Human Cell Line is detected by His tag Antibody.
(A) Recombinant SARS-Cov-2 Spike 1 His-tagged protein (10522-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP and was detected with Mouse Anti-His Allophycocyanin-conjugated Monoclonal Antibody (Catalog # IC050A). (B) No staining was observed in the absence of protein. Staining was performed using our Staining Membrane-Associated Proteins protocol.
Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Protein by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with His-tagged protein was stained with Mouse Anti-His Tag APC-conjugated Monoclonal Antibody (Catalog # IC050A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for His Tag APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: SARS-Cov-2 Spike 1 His tag protein binding to ACE-2-transfected Human Cell Line
Sample: SARS-Cov-2 Spike 1 His tag protein binding to ACE-2-transfected Human Cell Line
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with His-tagged protein, fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Sample: HEK293 human embryonic kidney cell line transfected with His-tagged protein, fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: His Tag
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.
Long Name
Histidine Tag
Alternate Names
polyHistidine
Additional His Tag Products
Product Documents for His Tag APC-conjugated Antibody
Product Specific Notices for His Tag APC-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...