Skip to main content

Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # AD1.1.10

Product Summary for His Tag Biotinylated Antibody

Immunogen

His-tagged peptide

Specificity

Detects proteins containing accessible consecutive histidine regions. This antibody detects His tags localized at the amino- or carboxyl-terminus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for His Tag Biotinylated Antibody

Detection of His Tag antibody by Western Blot.

Detection of His Tag by Western Blot.

Western blot shows lysates of HEK293T human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged BAI-1. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-His Tag Biotinylated Monoclonal Antibody (Catalog # BAM050) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for His Tag Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: HEK293T human embryonic kidney cell line and CHO Chinese hamster ovary cell line transfected with His-tagged proteins
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 6 reviews rated 4.2 using BAM050 in the following applications:

Published Applications

Read 8 publications using BAM050 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name

Histidine Tag

Alternate Names

polyHistidine

Product Documents for His Tag Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for His Tag Biotinylated Antibody

For research use only

Loading...
Loading...

FAQs for His Tag Biotinylated Antibody

Showing  1 - 1 of 1 FAQ Showing All
  • Q: Can the His Tag Antibody (MAB050) recognize all His-tagged proteins?

    A: MAB050 has been validated specifically for the detection of His-tags localized to the amino- or carboxyl-terminus of a peptide.  It is also expected to detect any series of 6 or more histidine residues as long as this epitope is conformationally accessible. For applications utilizing linearized proteins, it is likely that MAB050 will have access to any consecutive histidine regions.  If used with protein in its native conformation, there is the potential that the protein will be folded such that the antibody is not able to bind its target.

Showing  1 - 1 of 1 FAQ Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Loading...
Setting the standard in quality research reagents