This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

Name: Recombinant Human TRAIL/TNFSF10 Protein
Brand: R&D Systems
SKU: 375-TL
Rating: 5 (1 reviews)

The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

Recombinant Human TRAIL/TNFSF10 Protein Best Seller

Catalog # 375-TL | R&D Systems, Inc. a Bio-Techne Brand

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Citations (52)

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Key Product Details

Source:	NS0
Accession #:	P50591
Structure / Form:	Noncovalently-linked homotrimer and disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human TRAIL/TNFSF10 Protein

Technical Data
Citations (52) / Reviews (1) / FAQs

Product Specifications for Recombinant Human TRAIL/TNFSF10 Protein

Source	Mouse myeloma cell line, NS0-derived human TRAIL/TNFSF10 protein Thr95-Gly281, with an N-terminal Met and 6-His tag
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	21 kDa (monomer)
SDS-PAGE	21 kDa (monomer)
Activity	Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED₅₀ for this effect is 4-12 ng/mL. The apoptotic effect of soluble trimeric rhTRAIL can be enhanced by oligimerization of the soluble rhTRAIL through the use of a cross-linking antibody Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050). The ED₅₀ for this effect is 1-4 ng/mL. Note: This is one of multiple forms available for this protein. Check R&D Systems' website, www.RnDSystems.com, for a complete listing of the variants.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

375-TL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 20 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -70 °C under sterile conditions after reconstitution.

375-TL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRAIL/TNFSF10

TRAIL (TNF-related apoptosis-inducing ligand), also known as APO-2 ligand, is a type II transmembrane protein with a carboxy-terminal extracellular domain which exhibits homology to other TNF family members. In the new TNF superfamily nomenclature, TRAIL is referred to as TNFSF10. Human TRAIL cDNA encodes a 281 amino acid (aa) residue protein with an amino-terminal intracellular domain of 17 residues and a predicted internal hydrophobic domain between residues 18 and 38. The extracellular carboxy-terminal domain contains a potential N-linked glycosylation site at amino acid residue 109. Among TNF family members, TRAIL is most homologous to FAS ligand, sharing 28% amino acid sequence identity in their extracellular domains. Mouse TRAIL has also been cloned. The human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells. Both membrane bound and soluble TRAIL have been shown to induce rapid apoptosis of many transformed cell lines. Like most TNF family members, the bioactive TRAIL exists as a homotrimer. TRAIL transcripts have been shown to be constitutively expressed in a variety of human tissues. A family of TRAIL receptors, including two receptors that tranduce the apoptotic signals and two TRAIL decoy receptors that function to antagonize TRAIL-induced apoptosis, have been identified (1 - 3). Osteoprotegerin has been identified as a fifth TRAIL receptor (4).

Item	Value
References	Golstein, P. (1997) Current Biology 7:R750. Wiley, S.R. et al. (1995) Immunity 3:673. Pitti, R.M. et al. (1996) J. Biol. Chem. 271:12687. Emery, J. et al. (1998) J. Biol. Chem. 273:14363.
Long Name	TNF-related Apoptosis-inducing Ligand
Entrez Gene IDs	8743 (Human); 22035 (Mouse); 246775 (Rat)
Alternate Names	APO2L, APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta, Apo-2 ligand, Apo-2Ltumor necrosis factor (ligand) family, member 10, CD253, CD253 antigen, Protein TRAIL, TL2, TNF-related apoptosis-inducing ligand, TNFSF10, TRAIL, TRAILTNF-related apoptosis inducing ligand TRAIL, tumor necrosis factor (ligand) superfamily, member 10, tumor necrosis factor ligand superfamily member 10

Citations for Recombinant Human TRAIL/TNFSF10 Protein (52)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human TRAIL/TNFSF10 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Shlyakhtin et al. (2023-02-25), BdLT-Seq as a barcode decay-based method to unravel lineage-linked transcriptome plasticity Nature Communications, 2023-014(1):1085.
PMID: 36841849
Species: Complex Species Category
Sample Types: Whole Cells
Applications: Bioassay

Bhuyan S et al. (2022-09-29), Targeting hypoxia-induced tumor stemness by activating pathogen-induced stem cell niche defense. Front Immunol, 2022-013(0):933329.
PMID: 36248858
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SM Woo et al. (2022-09-24), Inhibition of USP1 enhances anticancer drugs-induced cancer cell death through downregulation of survivin and miR-216a-5p-mediated upregulation of DR5 Cell Death Disease, 2022-09-24;13(9):821.
PMID: 36153316
Species: Human
Sample Types: Transduced Whole Cells
Applications: Bioassay

J Joung et al. (2022-03-25), CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity Nature Communications, 2022-013(1):1606.
PMID: 35338135
Species: Human
Sample Types: Whole Cell
Applications: Bioassay

BT Wang et al. (2021-10-28), Multimeric Anti-DR5 IgM Agonist Antibody IGM-8444 Is a Potent Inducer of Cancer Cell Apoptosis and Synergizes with Chemotherapy and BCL-2 Inhibitor ABT-199 Molecular Cancer Therapeutics, 2021-10(0).
PMID: 34711645
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

M Lee et al. (2020-11-10), Dipeptidyl peptidase-4 inhibitor protects against non-alcoholic steatohepatitis in mice by targeting TRAIL receptor-mediated lipoapoptosis via modulatingï¿½hepatic dipeptidyl peptidase-4 expression Sci Rep, 2020-110(1):19429.
PMID: 33173107
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

K Landgraf et al. (2020-10-20), The Obesity-Susceptibility Gene TMEM18 Promotes Adipogenesis through Activation of PPARG Cell Rep, 2020-133(3):108295.
PMID: 33086065
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

H Forde et al. (2020-10-01), TRAIL inhibits oxidative stress in human aortic endothelial cells exposed to pro-inflammatory stimuli Physiol Rep, 2020-18(20):e14612.
PMID: 33080110
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

SM Woo et al. (2019-12-06), Honokiol Enhances TRAIL-Mediated Apoptosis through STAMBPL1-Induced Survivin and c-FLIP Degradation Biomolecules, 2019-19(12).
PMID: 31817770
Species: Human, Xenograft
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo

M Hong et al. (2019-11-19), Acetylshikonin Sensitizes Hepatocellular Carcinoma Cells to Apoptosis through ROS-Mediated Caspase Activation Cells, 2019-18(11).
PMID: 31752383
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SP O'Hara et al. (2019-10-28), The transcription factor ETS1 promotes apoptosis resistance of senescent cholangiocytes by epigenetically up-regulating the apoptosis suppressor BCL2L1 J. Biol. Chem., 2019-10(0).
PMID: 31659122
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

WY Kong et al. (2019-09-19), Zebularine and trichostatin A sensitized human breast adenocarcinoma cells towards tumor necrosis factor-related apoptosis inducing ligand (TRAIL)-induced apoptosis Heliyon, 2019-05(9):e02468.
PMID: 31687564
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

EA Kim et al. (2019-07-19), MiR-1208 Increases the Sensitivity to Cisplatin by Targeting TBCK in Renal Cancer Cells Int J Mol Sci, 2019-020(14).
PMID: 31331056
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SM Woo et al. (2019-07-02), Axl Inhibitor R428 Enhances TRAIL-Mediated Apoptosis Through Downregulation of c-FLIP and Survivin Expression in Renal Carcinoma Int J Mol Sci, 2019-020(13).
PMID: 31269715
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

EB Jo et al. (2019-05-24), Combination therapy with c-met inhibitor and TRAIL enhances apoptosis in dedifferentiated liposarcoma patient-derived cells BMC Cancer, 2019-019(1):496.
PMID: 31126284
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Li et al. (2019-02-12), Activated NK cells kill hepatic stellate cells via p38/PI3K signaling in a TRAIL-involved degranulation manner J. Leukoc. Biol., 2019-00(0).
PMID: 30748035
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

BR Kim et al. (2019-01-28), RUNX3 enhances TRAIL-induced apoptosis by upregulating DR5 in colorectal cancer Oncogene, 2019-00(0).
PMID: 30692634
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Tian et al. (2018-11-16), Aldehyde Dehydrogenase 1 (ALDH1) Promotes the Toxicity of TRAIL in Non-Small Cell Lung Cancer Cells via Post-Transcriptional Regulation of MEK-1 Expression Cell. Physiol. Biochem., 2018-151(1):217-227.
PMID: 30448845
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

B Lian et al. (2018-09-26), miR-128 Targets the SIRT1/ROS/DR5 Pathway to Sensitize Colorectal Cancer to TRAIL-Induced Apoptosis Cell. Physiol. Biochem., 2018-049(6):2151-2162.
PMID: 30257253
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

CJ Williams et al. (2018-03-22), Mutations in osteoprotegerin account for the CCAL1 locus in calcium pyrophosphate deposition disease Osteoarthr. Cartil., 2018-00(0).
PMID: 29578045
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR

AW Clarke et al. (2018-03-05), An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease MAbs, 2018-00(0):1-43.
PMID: 29436901
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

DY Liang et al. (2018-02-14), ShDcR3 sensitizes TRAIL-resistant HCC cells by inducing caspase-dependent apoptosis while suppressing NF-?B dependent cFLIPL expression PLoS ONE, 2018-013(2):e0191545.
PMID: 29444104
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SH Hong et al. (2018-02-09), Silencing of peroxiredoxin II by promoter methylation is necessary for the survival and migration of gastric cancer cells Exp. Mol. Med., 2018-050(2):e443.
PMID: 29422545
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

P Zhang et al. (2017-12-12), Targeting CDK1 and MEK/ERK Overcomes Apoptotic Resistance in BRAF Mutated human Colorectal Cancer Mol. Cancer Res., 2017-10(0).
PMID: 29233910
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SM Woo et al. (2017-11-20), Up-regulation of 5-lipoxygenase by inhibition of cathepsin G enhances TRAIL-induced apoptosis through down-regulation of survivin Oncotarget, 2017-18(63):106672-106684.
PMID: 29290980
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MJ Mitchell et al. (2017-03-20), Polymeric mechanical amplifiers of immune cytokine-mediated apoptosis Nat Commun, 2017-08(0):14179.
PMID: 28317839
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Bo Ra You et al. (2017-03-14), Suberoylanilide hydroxamic acid increases anti-cancer effect of tumor necrosis factor-? through up-regulation of TNF receptor 1 in lung cancer cells. Oncotarget, 2017-00(0):1949-2553.
PMID: 28099148
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Yang et al. (2017-03-01), Chemoresistance is associated with overexpression of HAX-1, inhibition of which resensitizes drug-resistant breast cancer cells to chemotherapy Tumour Biol, 2017-039(3):1010428317692.
PMID: 28347249
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Liang et al. (2017-01-18), Downregulation of DcR3 sensitizes hepatocellular carcinoma cells to TRAIL-induced apoptosis Onco Targets Ther, 2017-010(0):417-428.
PMID: 28176915
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SH Lee et al. (2016-10-27), Potential Role of CD133 Expression in the Susceptibility of Human Liver Cancer Stem-Like Cells to TRAIL Oncol. Res., 2016-124(6):495-509.
PMID: 28281970
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-04-19), TNFSF10/TRAIL regulates human T4 effector memory lymphocyte radiosensitivity and predicts radiation-induced acute and subacute dermatitis Oncotarget, 2016-07(16):21416-27.
PMID: 26982083
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lizarraga F et al. (2015-08-20), Tissue Inhibitor of Metalloproteinase-4 Triggers Apoptosis in Cervical Cancer Cells. PLoS ONE, 2015-010(8):e0135929.
PMID: 26291714
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Meng X et al. (2014-07-25), Poly(ADP-ribose) polymerase inhibitors sensitize cancer cells to death receptor-mediated apoptosis by enhancing death receptor expression. J Biol Chem, 2014-0289(30):20543-58.
PMID: 24895135
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Jang et al. (2014-06-13), TRAIL enhances apoptosis of human hepatocellular carcinoma cells sensitized by hepatitis C virus infection: therapeutic implications. PLoS ONE, 2014-09(6):e98171.
PMID: 24927176
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Mathieu J et al. (2014-03-20), Hypoxia-inducible factors have distinct and stage-specific roles during reprogramming of human cells to pluripotency. Cell Stem Cell, 2014-014(5):592-605.
PMID: 24656769
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

You B et al. (2014-03-12), Suberoylanilide hydroxamic acid-induced HeLa cell death is closely correlated with oxidative stress and thioredoxin 1 levels. Int J Oncol, 2014-044(5):1745-55.
PMID: 24626405
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wehrkamp et al. (2014-03-06), XIAP antagonist embelin inhibited proliferation of cholangiocarcinoma cells. PLoS ONE, 2014-09(3):e90238.
PMID: 24603802
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kim et al. (2014-01-16), Potential application of temozolomide in mesenchymal stem cell-based TRAIL gene therapy against malignant glioma. Stem Cells Transl Med, 2014-03(2):172-82.
PMID: 24436439
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Yang et al. (2013-12-02), Inhibition of PP2A activity confers a TRAIL-sensitive phenotype during malignant transformation. Mol Cancer Res, 2013-112(2):217-27.
PMID: 24296757
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tatsuta T et al. (2013-11-28), Sialic acid-binding lectin (leczyme) induces apoptosis to malignant mesothelioma and exerts synergistic antitumor effects with TRAIL. Int J Oncol, 2013-144(2):377-84.
PMID: 24297392
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

De Chiara A et al. (2013-07-03), Characterization of cytosolic proliferating cell nuclear antigen (PCNA) in neutrophils: antiapoptotic role of the monomer. J Leukoc Biol, 2013-094(4):723-31.
PMID: 23825390
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Akimoto et al. (2013-02-04), Umbilical cord blood-derived mesenchymal stem cells inhibit, but adipose tissue-derived mesenchymal stem cells promote, glioblastoma multiforme proliferation. Stem Cells Dev, 2013-022(9):1370-86.
PMID: 23231075
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012-07-26), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-0487(7408):505-9.
PMID: 22763448
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Sagiv et al. (2012-07-02), Granule exocytosis mediates immune surveillance of senescent cells. Oncogene, 2012-032(15):1971-7.
PMID: 22751116
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kang Z et al. (2011-03-08), Drozitumab, a human antibody to death receptor 5, has potent antitumor activity against rhabdomyosarcoma with the expression of caspase-8 predictive of response. Clin Cancer Res, 2011-017(10):3181-92.
PMID: 21385927
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Vanhecke E et al. (2011-02-24), Brain-derived neurotrophic factor and neurotrophin-4/5 are expressed in breast cancer and can be targeted to inhibit tumor cell survival. Clin. Cancer Res., 2011-017(7):1741-52.
PMID: 21350004
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Park SJ et al. (2009-01-24), Trichostatin A sensitizes human ovarian cancer cells to TRAIL-induced apoptosis by down-regulation of c-FLIPL via inhibition of EGFR pathway. Biochem. Pharmacol., 2009-077(8):1328-36.
PMID: 19426671
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Chamoux E et al. (2008-08-01), Osteoprotegerin decreases human osteoclast apoptosis by inhibiting the TRAIL pathway. J. Cell. Physiol., 2008-0216(2):536-42.
PMID: 18338379
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Inglefield JR et al. (2008-04-01), TLR7 Agonist 852A Inhibition of Tumor Cell Proliferation Is Dependent on Plasmacytoid Dendritic Cells and Type I IFN. J. Interferon Cytokine Res., 2008-028(4):253-63.
PMID: 18439103
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Locklin RM et al. (2007-12-07), Selective targeting of death receptor 5 circumvents resistance of MG-63 osteosarcoma cells to TRAIL-induced apoptosis. Mol. Cancer Ther., 2007-16(12):3219-28.
PMID: 18065493
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay

Dumitru CA et al. (2006-04-24), TRAIL activates acid sphingomyelinase via a redox mechanism and releases ceramide to trigger apoptosis. Oncogene, 2006-025(41):5612-25.
PMID: 16636669
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Cross SS et al. (2006-04-15), Osteoprotegerin (OPG)--a potential new role in the regulation of endothelial cell phenotype and tumour angiogenesis? Int. J. Cancer, 2006-0118(8):1901-8.
PMID: 16287088

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Customer Reviews for Recombinant Human TRAIL/TNFSF10 Protein (1)

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Recombinant Human TRAIL/TNFSF10 Protein

Name: Sijie Bi

Application: Cell Viability Assay

Reason for Rating: Results met expectation

Verified Customer | Posted 07/21/2016

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FAQs for Recombinant Human TRAIL/TNFSF10 Protein

This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

Recombinant Human TRAIL/TNFSF10 Protein Best Seller

Catalog # 375-TL | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human TRAIL/TNFSF10 Protein

Formulation, Preparation and Storage

Carrier Free

375-TL

Carrier

375-TL/CF

Carrier Free

Reconstitution Calculator

Background: TRAIL/TNFSF10

Citations for Recombinant Human TRAIL/TNFSF10 Protein (52)

Customer Reviews for Recombinant Human TRAIL/TNFSF10 Protein (1)

Customer Images

Recombinant Human TRAIL/TNFSF10 Protein

FAQs for Recombinant Human TRAIL/TNFSF10 Protein

Product Documents for Recombinant Human TRAIL/TNFSF10 Protein

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SDS

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