Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

Name: Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody
Brand: R&D Systems
SKU: MAB1536
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1536

Citations (37)

(2)

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Key Product Details

Species Reactivity:	Human, Mouse, Rat
Immunogen:	E. coli-derived recombinant human HIF-1 alpha/HIF1A Arg575-Asn826 Accession # Q16665.1
Applications:	Immunohistochemistry, Immunoprecipitation, Knockout Validated, Simple Western, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 241809
Specificity:	Detects human, mouse, and rat HIF-1 alpha/HIF1A.

View Full Technical Details for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

Applications for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	5-25 µg/mL	See below
Immunoprecipitation	1-3 µg/500 µg cell lysate	MCF‑7 human breast cancer cell line treated with CoCl₂, see our available Western blot detection antibodies

Item	Value
Knockout Validated	HIF-1 alpha/HIF1A is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in HIF-1 alpha/HIF1A knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

	Western Blot Detection of Human, Mouse, and Rat HIF-1 alpha/HIF1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Balb-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl₂for 8 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Monoclonal Antibody, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HIF-1 alpha/HIF1A at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry HIF-1 alpha/HIF1A in Human Kidney. HIF-1 alpha/HIF1A was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB1536) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Knockout Validated Western Blot Shows Human HIF-1 alpha/HIF1A alpha/HIF1A Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha/HIF1A knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO overnight. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB1536) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha/HIF1A at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Simple Western Detection of Human HIF-1 alpha/HIF1A by Simple Western^TM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O₂), loaded at 0.2 mg/mL. A specific band was detected for HIF-1 alpha/HIF1A at approximately 116 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB1536). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	Knockout Validated HIF-1 alpha/HIF1A Specificity is Shown by Immunocytochemistry in Knockout Cell Line. HIF-1 alpha/HIF1A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO but is not detected in HIF-1 alpha/HIF1A knockout (KO) HeLa Human Cell Line cell line using Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A alpha/HIF1A Monoclonal Antibody (Catalog # MAB1536) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: HIF-1 alpha/HIF1A

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Item	Value
Long Name	Hypoxia Inducible Factor 1 Subunit Alpha
Entrez Gene IDs	3091 (Human); 15251 (Mouse); 29560 (Rat)
Alternate Names	AINT, ARNT interacting protein, ARNT-interacting protein, BHLHE78, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, HIF 1A, HIF-1 alpha, HIF-1-alpha, HIF-1a, HIF-1alpha, HIF1, HIF1 alpha, HIF1-alpha, HIF1A, MOP1, Member of PAS protein 1, PAS domain-containing protein 8, PASD8, anti-HIF-1 alpha, anti-HIF1A, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor 1-alpha, member of PAS superfamily 1

Citations for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody (37)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody include:

Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot

MF Pittenger et al. (2022), MSC Pretreatment for Improved Transplantation Viability Results in Improved Ventricular Function in Infarcted Hearts International Journal of Molecular Sciences, 23(2).
PMID: 35054878
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

AN Jiang et al. (2022), The study of direct and indirect effects of radiofrequency ablation on tumor microenvironment in liver tumor animal model BMC Cancer, 22(1):663.
PMID: 35710408
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

A Pietrobon et al. (2022), Renal organoid modeling of tuberous sclerosis complex reveals lesion features arise from diverse developmental processes Cell Reports, 40(1):111048.
PMID: 35793620
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Y Zhang et al. (2022), Stearoyl-CoA Desaturase-1 dependent lipid droplets accumulation in cancer-associated fibroblasts facilitates the progression of lung cancer International journal of biological sciences, 18(16):6114-6128.
PMID: 36439884
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot

S Lim et al. (2021), mTORC1-induced retinal progenitor cell overproliferation leads to accelerated mitotic aging and degeneration of descendent Mï¿½ller glia Elife, 10(0).
PMID: 34677125
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

K Taniguchi et al. (2021), &gamma-Glutamylcyclotransferase, a novel regulator of HIF-1&alpha expression, triggers aerobic glycolysis Cancer Gene Therapy, 0(0).
PMID: 33402732
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

K Kumon et al. (2021), Differentiation of cancer stem cells into erythroblasts in the presence of CoCl2 Scientific Reports, 11(1):23977.
PMID: 34907219
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

CM Edwards et al. (2021), HDAC inhibitors stimulate LIFR when it is repressed by hypoxia or PTHrP in breast cancer Oncogene, 31(0):100407.
PMID: 34934614
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

J Sevilla-Mo et al. (2020), pVHL-mediated regulation of the anti-angiogenic protein thrombospondin-1 decreases migration of Clear Cell Renal Carcinoma Cell Lines Sci Rep, 10(1):1175.
PMID: 31980715
Species: Rat
Sample Types: Cell Lysate
Applications: Western Blot

K Toriuchi et al. (2020), Prolonged astrocyte-derived erythropoietin expression attenuates neuronal damage under hypothermic conditions J Neuroinflammation, 17(1):141.
PMID: 32359362
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

GI Gavriilidi et al. (2020), Stem cell factor Is implicated In microenvironmental interactions and cellular dynamics of chronic lymphocytic leukemia Haematologica, 0(0).
PMID: 32336682
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

S Rattila et al. (2019), Interaction of Pregnancy-Specific Glycoprotein 1 With Integrin alpha5beta1 Is a Modulator of Extravillous Trophoblast Functions Cells, 8(11).
PMID: 31683744
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

S Jalili-Fir et al. (2018), Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip Cell Death Dis, 9(2):223.
PMID: 29445080
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

M van Gijsel et al. (2017), Metabolic changes and inflammation in cultured astrocytes from the 5xFAD mouse model of Alzheimer's disease: Alleviation by pantethine PLoS ONE, 12(4):e0175369.
PMID: 28410378
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

HD Basavaraja et al. (2017), Ferrochelatase is a therapeutic target for ocular neovascularization EMBO Mol Med, 0(0).
PMID: 28377496
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

P Wang et al. (2017), HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia Oncotarget, 8(17):28074-28092.
PMID: 28427209
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

D Labrousse- et al. (2017), VHL promotes immune response against renal cell carcinoma via NF-?B-dependent regulation of VCAM-1 J. Cell Biol, 0(0).
PMID: 28235946
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

CV Park et al. (2017), XIAP upregulates expression of HIF target genes by targeting HIF1? for Lys63-linked polyubiquitination Nucleic Acids Res., 0(0).
PMID: 28666324
Species: Human
Sample Types: Protein
Applications: Western Blot

AP Fischer et al. (2017), Silencing HIF-1? induces TET2 expression and augments ascorbic acid induced 5-hydroxymethylation of DNA in human metastatic melanoma cells Biochem. Biophys. Res. Commun., 0(0).
PMID: 28601635
Species: Bovine
Sample Types: Cell Lysates
Applications: Western Blot

P Hernansanz et al. (2017), Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia Redox Biol, 12(0):1040-1051.
PMID: 28511347
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

M Sone et al. (2017), Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency Cell Metab., 25(5):1103-1117.e6.
PMID: 28467928
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

K Rao et al. (2017), Protective effect of zinc preconditioning against renal ischemia reperfusion injury is dose dependent PLoS ONE, 12(7):e0180028.
PMID: 28686686
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

(2016), Leptin directly promotes T-cell glycolytic metabolism to drive effector T-cell differentiation in a mouse model of autoimmunity Eur J Immunol, 0(0).
PMID: 27222115
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Sagar Bhogaraju (2016), Phosphoribosylation of Ubiquitin Promotes Serine Ubiquitination and Impairs Conventional Ubiquitination Cell, 167(6):1636-1649.e13.
PMID: 27912065
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot

Qin Wei (2016), Chronic intermittent hypoxia induces cardiac inflammation and dysfunction in a rat obstructive sleep apnea model J Biomed Res, 30(6):490-495.
PMID: 27924067
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

(2016), NQO1 inhibits proteasome-mediated degradation of HIF-1? Nat Commun, 7(0):13593.
PMID: 27966538
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Sun Y et al. (2015), Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia. PLoS ONE, 10(4):e0123649.
PMID: 25830774
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr

Fischer C et al. (2015), Panobinostat reduces hypoxia-induced cisplatin resistance of non-small cell lung carcinoma cells via HIF-1alpha destabilization. Mol Cancer, 14(1):4.
PMID: 25608569
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Fujita N et al. (2014), HIF-1-PHD2 axis controls expression of syndecan 4 in nucleus pulposus cells. FASEB J, 28(6):2455-65.
PMID: 24558194
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Bruzzese L et al. (2014), NF-kappaB enhances hypoxia-driven T-cell immunosuppression via upregulation of adenosine A(2A) receptors. Cell Signal, 26(5):1060-7.
PMID: 24486403
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Bett J et al. (2013), The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability. Biochem J, 451(2):185-94.
PMID: 23398456
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

van Uden P et al. (2012), Evolutionary conserved regulation of HIF-1beta by NF-kappaB. PLoS Genet., 7(1):e1001285.
PMID: 21298084
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Liu H et al. (2010), Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1alpha in MSCs. Biochem. Biophys. Res. Commun., 401(4):509-15.
PMID: 20869949
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Hickey MM et al. (2010), The von Hippel-Lindau Chuvash mutation promotes pulmonary hypertension and fibrosis in mice. J. Clin. Invest., 120(3):827-39.
PMID: 20197624
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Zahm AM et al. (2008), Oxygen tension regulates preosteocyte maturation and mineralization. Bone, 43(1):25-31.
PMID: 18485858
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Monnier Y et al. (2008), CYR61 and alphaVbeta5 integrin cooperate to promote invasion and metastasis of tumors growing in preirradiated stroma. Cancer Res., 68(18):7323-31.
PMID: 18794119
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Risbud MV et al. (2006), Nucleus pulposus cells express HIF-1alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment. J. Cell. Biochem., 98(1):152-9.
PMID: 16408279

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Customer Reviews for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody (2)

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Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

Name: Anonymous

Application: IHC

Sample Tested: Brain tissue

Species: Mouse

Verified Customer | Posted 06/23/2022
Human/Mouse/Rat HIF-1 alpha Antibody

Name: Anonymous

Application: WB

Sample Tested: Caco-2 human colorectal adenocarcinoma cell line

Species: Human

Verified Customer | Posted 08/14/2017

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Product Documents for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

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Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

Scientific Data Examples for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

Western Blot

Immunohistochemistry

Knockout Validated

Simple Western

Knockout Validated

Formulation, Preparation and Storage

Reconstitution Calculator

Background: HIF-1 alpha/HIF1A

Citations for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody (37)

Customer Reviews for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody (2)

Customer Images

Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

Human/Mouse/Rat HIF-1 alpha Antibody

Product Documents for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

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