Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

Name: Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody
Brand: R&D Systems
SKU: AF1935
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1935

Citations (26)

(1)

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Species Reactivity:	Human, Mouse, Rat
Immunogen:	E. coli-derived recombinant human HIF-1 alpha /HIF1A Arg575-Asn826 Accession # Q16665.1
Applications:	Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Knockout Validated, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human, mouse and rat HIF-1 alpha /HIF1A.

View Full Technical Details for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

Applications for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5 µg/mL	See below
Immunoprecipitation	2 µg/500 µg cell lysate	MCF‑7 human breast cancer cell line treated with CoCl₂, see our available Western blot detection antibodies
Chromatin Immunoprecipitation (ChIP)	5 µg/5 x 10⁶ cells	See below
Immunocytochemistry	0.3-15 µg/mL	See below

Item	Value
Knockout Validated	HIF-1 alpha /HIF1A is specifically detected in HeLa human cervical epithelial carcinoma cell line but is not detectable in HIF-1 alpha/HIF1A knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

	Western Blot Detection of Human HIF‑1 alpha /HIF1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl₂for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen-affinity Purified Polyclonal Antibody, followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF-1a/HIF1A at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Chromatin Immunoprecipitation (ChIP) Detection of HIF‑1 alpha /HIF1A-regulated Genes by Chromatin Immunoprecipitation. Mouse primary kidney cells treated with 150 µM CoCl2 for overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HIF-1a/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The epo promoter was detected by standard PCR.
	Immunocytochemistry HIF‑1 alpha /HIF1A Specificity is Shown by Immunocytochemistry in Knockout Cell Line. HIF-1a/HIF1A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO but is not detected in HIF-1a/HIF1A knockout (KO) HeLa Human Cell Line cell line using Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Simple Western Detection of Human HIF‑1 alpha /HIF1A by Simple Western^TM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O₂), loaded at 0.2 mg/mL. A specific band was detected for HIF-1a/HIF1A at approximately 115 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: HIF-1 alpha/HIF1A

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Item	Value
Long Name	Hypoxia Inducible Factor 1 Subunit Alpha
Entrez Gene IDs	3091 (Human); 15251 (Mouse); 29560 (Rat)
Alternate Names	AINT, ARNT interacting protein, ARNT-interacting protein, BHLHE78, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, HIF 1A, HIF-1 alpha, HIF-1-alpha, HIF-1a, HIF-1alpha, HIF1, HIF1 alpha, HIF1-alpha, HIF1A, MOP1, Member of PAS protein 1, PAS domain-containing protein 8, PASD8, anti-HIF-1 alpha, anti-HIF1A, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor 1-alpha, member of PAS superfamily 1

Citations for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody (26)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

G Zeng et al. (2021), FLI1 mediates the selective expression of hypoxia-inducible factor-1 target genes in endothelial cells under hypoxic conditions FEBS Open Bio, 0(0).
PMID: 34102031
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

X Fan et al. (2021), LncRNA-SNHG6 promotes the progression of hepatocellular carcinoma by targeting miR-6509-5p and HIF1A Cancer Cell International, 21(1):150.
PMID: 33663502
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

T Kjeld et al. (2021), Extreme Hypoxia Causing Brady-Arrythmias During Apnea in Elite Breath-Hold Divers Frontiers in Physiology, 12(0):712573.
PMID: 34925050
Species: Mouse
Sample Types: Brain Section
Applications: IHC-F

M Felsenstei et al. (2020), CCR2 of Tumor Microenvironmental Cells Is a Relevant Modulator of Glioma Biology Cancers (Basel), 12(7).
PMID: 32668709
Species: Mouse
Sample Types: Chromatin
Applications: Immunoprecipitation (ChIP)

X Li et al. (2020), The critical role of MMP14 in adipose tissue remodeling during obesity Mol. Cell. Biol., 0(0).
PMID: 31988105
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

B Di Marco et al. (2020), Reciprocal Interaction between Vascular Filopodia and Neural Stem Cells Shapes Neurogenesis in the Ventral Telencephalon Cell Rep, 33(2):108256.
PMID: 33053356
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

K Tawara et al. (2018), Co-Expression of VEGF and IL-6 Family Cytokines is Associated with Decreased Survival in HER2 Negative Breast Cancer Patients: Subtype-Specific IL-6 Family Cytokine-Mediated VEGF Secretion Transl Oncol, 12(2):245-255.
PMID: 30439625
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

AN Billin et al. (2018), HIF prolyl hydroxylase inhibition protects skeletal muscle from eccentric contraction-induced injury Skelet Muscle, 8(1):35.
PMID: 30424786
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Q Yang et al. (2018), PRKAA1/AMPK?1-driven glycolysis in endothelial cells exposed to disturbed flow protects against atherosclerosis Nat Commun, 9(1):4667.
PMID: 30405100
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

X Li et al. (2017), Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells Sci Rep, 7(1):5081.
PMID: 28698547
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

P Himmels et al. (2017), Motor neurons control blood vessel patterning in the developing spinal cord Nat Commun, 8(0):14583.
PMID: 28262664
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Z Liu et al. (2017), Endothelial adenosine A2a receptor-mediated glycolysis is essential for pathological retinal angiogenesis Nat Commun, 8(1):584.
PMID: 28928465
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

L Li et al. (2017), Forkhead Box O3 (FoxO3) Regulates Kidney Tubular Autophagy Following Urinary Tract Obstruction J. Biol. Chem., 0(0).
PMID: 28705935
Species: Human
Sample Types: Chromatin
Applications: ChIP

(2016), HEREGULIN/ErbB3 SIGNALING ENHANCES CXCR4-DRIVEN Rac1 ACTIVATION AND BREAST CANCER CELL MOTILITY VIA HIF-1? Mol Cell Biol, 0(0).
PMID: 27185877
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Gabi U Dachs (2016), Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo(-/-) mice Hypoxia (Auckl), 4(0):41-52.
PMID: 27800507
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

(2016), Identification of ?-Dystrobrevin as a Direct Target of miR-143: Involvement in Early Stages of Neural Differentiation PLoS ONE, 11(5):e0156325.
PMID: 27223470
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Tholen M et al. (2015), Stress-resistant Translation of Cathepsin L mRNA in Breast Cancer Progression. J Biol Chem, 290(25):15758-69.
PMID: 25957406
Species: Rat
Sample Types: Cell Lysates
Applications: ChIP

Li R et al. (2015), High Glucose Up-regulates ADAM17 through HIF-1alpha in Mesangial Cells. J Biol Chem, 290(35):21603-14.
PMID: 26175156
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Zuo R et al. (2015), Warburg-like Glycolysis and Lactate Shuttle in Mouse Decidua during Early Pregnancy. J Biol Chem, 290(35):21280-91.
PMID: 26178372
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ChIP

Palladino M et al. (2012), Myeloid cell leukemia-1 (Mc1-1) is a candidate target gene of hypoxia-inducible factor-1 (HIF-1) in the testis. Reprod Biol Endocrinol, 10(0):104.
PMID: 23216940
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Liang D et al. (2012), The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells. BMC Cancer, 12(0):201.
PMID: 22642602
Species: Mouse
Sample Types: Whole Cells
Applications: Western Blot

Joshi D et al. (2011), Development of an in vitro model of myotube ischemia. Lab. Invest., 91(8):1241-52.
PMID: 21606923
Species: Human
Sample Types: Cell Lysates
Applications: ChIP

Robador PA et al. (2011), HIF-1-mediated up-regulation of cardiotrophin-1 is involved in the survival response of cardiomyocytes to hypoxia. Cardiovasc. Res., 92(2):247-55.
PMID: 21771897
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Bollig-Fischer A et al. (2010), HER-2 signaling, acquisition of growth factor independence, and regulation of biological networks associated with cell transformation. Cancer Res., 70(20):7862-73.
PMID: 20736364
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Wang B et al. (2008), Hypoxia induces leptin gene expression and secretion in human preadipocytes: differential effects of hypoxia on adipokine expression by preadipocytes. J. Endocrinol., 198(1):127-34.
PMID: 18463145
Species: Human
Sample Types: Nuclear Extract
Applications: Western Blot

Colla S et al. (2007), The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha (HIF-1alpha) activity: involvement in myeloma-induced angiogenesis. Blood, 110(13):4464-75.
PMID: 17848618

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Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

Name: Anonymous

Sample Tested: HASMC human aortic smooth muscle cells

Species: Human

Verified Customer | Posted 12/04/2019

I have tried HIF1A antibodies from several companies, this one is good compared to others, but sometimes there are multiple bands. 1:500

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Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

Scientific Data Examples for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

Western Blot

Chromatin Immunoprecipitation (ChIP)

Immunocytochemistry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: HIF-1 alpha/HIF1A

Citations for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody (26)

Customer Reviews for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody (1)

Customer Images

Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

Product Documents for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

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