Mouse/Rat Neuropilin-1 Antibody

Name: Mouse/Rat Neuropilin-1 Antibody
Brand: R&D Systems
SKU: AF566
Rating: 4 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF566

Citations (50)

(2)

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Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Mouse, Rat
Immunogen:	Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1 Phe22-Ala810 (Lys811Arg), Ser829-Asp854 Accession # Q9QWJ9
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed.

View Full Technical Details for Mouse/Rat Neuropilin-1 Antibody

Applications for Mouse/Rat Neuropilin-1 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Flow Cytometry	2.5 µg/10⁶ cells	See below
Immunohistochemistry	3-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.3-1 µg/mL of this antibody will block 50% of the binding of 20 ng/mL of Recombinant Human VEGF₁₆₅ (Catalog # 293-VE) to immobilized Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566‑NNS) coated at 2 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding. Also, 1-5 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human Semaphorin 3A Fc Chimera (Catalog # 1250-S3) to immobilized Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-NNS) coated at 2 µg/mL (100 µL/well).
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse/Rat Neuropilin-1 Antibody

	Western Blot Detection of Recombinant Human, Mouse, and Rat Neuropilin‑1 by Western Blot. Western blot shows 25 ng of Recombinant Mouse Neuropilin-1 (Catalog # 5994-N1), Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) and Recombinant Human Neuropilin-1 (Catalog # 3870-N1). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Neuropilin-1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Flow Cytometry Detection of Neuropilin-1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
	Immunohistochemistry Neuropilin‑1 in Embryonic Rat Spinal Cord. Neuropilin-1 was detected in immersion fixed frozen sections of embryonic rat spinal cord (15 d.p.c.) using 5 µg/mL Goat Anti-Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
	Immunohistochemistry Neuropilin‑1 in Rat Spinal Cord. Neuropilin-1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). Specific staining was localized to the dorsal horn. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
	Immunohistochemistry Neuropilin‑1 in Rat Brain. Neuropilin-1 was detected in immersion fixed paraffin-embedded sections of rat brain (hippocampus) using Goat Anti-Mouse/Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Immunohistochemistry Neuropilin‑1 in Rat Brain. Neuropilin-1 was detected in immersion fixed paraffin-embedded sections of rat brain (thalamus) using Goat Anti-Mouse/Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Neuropilin-1

Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. The amino acid sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.

Item	Value
Entrez Gene IDs	8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names	BDCA-4, BDCA4, CD304, CD304 antigen, DKFZp686A03134, DKFZp781F1414, NP1, NRP, NRP1, Neuropilin-1, Neuropilin1, VEGF165R, Vascular endothelial cell growth factor 165 receptor, neuropilin 1, transmembrane receptor

Product Specific Notices for Mouse/Rat Neuropilin-1 Antibody

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

Citations for Mouse/Rat Neuropilin-1 Antibody (50)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse/Rat Neuropilin-1 Antibody include:

Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Fantin et al. (2104), Neuropilin 1 (NRP1) hypomorphism combined with defective VEGF-A binding reveals novel roles for NRP1 in developmental and pathological angiogenesis. Development, 141(3):556-62.
PMID: 24401374
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

EMMA Andriessen et al. (2021), Myeloid-resident neuropilin-1 promotes choroidal neovascularization while mitigating inflammation Embo Molecular Medicine, 0(0):e11754.
PMID: 33876574
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

G Limoni et al. (2021), PlexinA4-Semaphorin3A-mediated crosstalk between main cortical interneuron classes is required for superficial interneuron lamination Cell Reports, 34(4):108644.
PMID: 33503438
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

CM Termini et al. (2021), Neuropilin 1 regulates bone marrow vascular regeneration and hematopoietic reconstitution Nature Communications, 12(1):6990.
PMID: 34848712
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

J Gonzales et al. (2020), Semaphorin 3A controls enteric neuron connectivity and is inversely associated with synapsin 1 expression in Hirschsprung disease Sci Rep, 10(1):15119.
PMID: 32934297
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

N Apóstolo et al. (2020), Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 11(1):5171.
PMID: 33057002
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

T Pemmari et al. (2020), Exposed CendR Domain in Homing Peptide Yields Skin-Targeted Therapeutic in Epidermolysis Bullosa Mol. Ther., 0(0).
PMID: 32497513
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

D Nilsson et al. (2019), Foxc2 is essential for podocyte function Physiol Rep, 7(9):e14083.
PMID: 31062503
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

CR Bye et al. (2019), Axonal growth of midbrain dopamine neurons is modulated by the cell adhesion molecule ALCAM through trans-heterophilic interaction with L1Cam, ChL1 and Semaphorin J. Neurosci., 0(0).
PMID: 31300520
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

MM Hossain et al. (2019), PlexinA1 is crucial for the midline crossing of callosal axons during corpus callosum development in BALB/cAJ mice PLoS ONE, 14(8):e0221440.
PMID: 31430342
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

DM Barry et al. (2019), Molecular determinants of nephron vascular specialization in the kidney Nat Commun, 10(1):5705.
PMID: 31836710
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

EZM Taroc et al. (2019), Gli3 regulates vomeronasal neurogenesis, olfactory ensheathing cell formation and GnRH-1 neuronal migration J. Neurosci., 0(0).
PMID: 31767679
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

AM Wilson et al. (2018), Neuropilin-1 expression in adipose tissue macrophages protects against obesity and metabolic syndrome Sci Immunol, 3(21).
PMID: 29549139
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

N Yang et al. (2017), Suppression of Retinal Neovascularization by Inhibition of Galectin-1 in a Murine Model of Oxygen-Induced Retinopathy J Ophthalmol, 2017(0):5053035.
PMID: 28428895
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

N Yang et al. (2017), Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy Exp. Eye Res, 0(0).
PMID: 28257831
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

H Lee et al. (2017), Rewiring the taste system Nature, 548(7667):330-333.
PMID: 28792937
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

K Inokuchi et al. (2017), Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odour-induced attractive social responses Nat Commun, 8(0):15977.
PMID: 28731029
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

P Fern ndez- et al. (2017), Neuropilin 1 Involvement in Choroidal and Retinal Neovascularisation PLoS ONE, 12(1):e0169865.
PMID: 28107458
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

JM Pinskey et al. (2017), Neuropilin-1 promotes Hedgehog signaling through a novel cytoplasmic motif J. Biol. Chem., 0(0).
PMID: 28667171
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Peter Mombaerts (2016), Neuropilin-1 and the Positions of Glomeruli in the Mouse Olfactory Bulb eNeuro, 3(5).
PMID: 27844052
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

(2016), NOVA2-mediated RNA regulation is required for axonal pathfinding during development Elife, 5(0).
PMID: 27223325
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Rama N et al. (2015), Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization. Nat Med, 21(5):483-91.
PMID: 25894826
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Plein A et al. (2015), Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation. J Clin Invest, 125(7):2661-76.
PMID: 26053665
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Tillo M et al. (2015), VEGF189 binds NRP1 and is sufficient for VEGF/NRP1-dependent neuronal patterning in the developing brain. Development, 142(2):314-9.
PMID: 25519242
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Giacobini P et al. (2014), Brain endothelial cells control fertility through ovarian-steroid-dependent release of semaphorin 3A. PLoS Biol, 12(3):e1001808.
PMID: 24618750
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Smolarchuk C et al. (2014), T cells generated in the absence of a thoracic thymus fail to establish homeostasis. Eur J Immunol, 44(8):2263-73.
PMID: 24777700
Species: Mouse
Sample Types: Whole Tissue
Applications: Functional Assay

Esquibies A et al. (2014), Heparin binding VEGF isoforms attenuate hyperoxic embryonic lung growth retardation via a FLK1-neuropilin-1-PKC dependent pathway. Respir Res, 15(0):32.
PMID: 24641672
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization

Molofsky A et al. (2014), Astrocyte-encoded positional cues maintain sensorimotor circuit integrity. Nature, 509(7499):189-94.
PMID: 24776795
Miyazaki et al. (2014), Id2 and Id3 maintain the regulatory T cell pool to suppress inflammatory disease. Nat Immunol, 15(8):767-76.
PMID: 24973820
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Shahrabi-Farahani et al. (2014), Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas. Lab Invest, 94(7):752-65.
PMID: 24791743
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Lee et al. (2014), Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing. Nat Immunol, 15(10):982-95.
PMID: 25173345
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Raimondi C et al. (2014), Imatinib inhibits VEGF-independent angiogenesis by targeting neuropilin 1-dependent ABL1 activation in endothelial cells. J Exp Med, 211(6):1167-83.
PMID: 24863063
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Petzold C et al. (2014), Fluorochrome-based definition of naturally occurring Foxp3(+) regulatory T cells of intra- and extrathymic origin. Eur J Immunol, 44(12):3632-45.
PMID: 25159127
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P

Ezkurdia N et al. (2014), Inhibition of neuronal apoptosis and axonal regression ameliorates sympathetic atrophy and hemodynamic alterations in portal hypertensive rats. PLoS ONE, 9(1):e84374.
PMID: 24400086
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation

Delgoffe et al. (2013), Stability and function of regulatory T cells is maintained by a neuropilin-1-semaphorin-4a axis. Nature, 501(7466):252-6.
PMID: 23913274
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

van Gils et al. (2013), Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions. Arterioscler Thromb Vasc Biol, 33(5):911-9.
PMID: 23430612
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Glinka Y et al. (2012), Neuropilin-1 is expressed by breast cancer stem-like cells and is linked to NF-kappaB activation and tumor sphere formation. Biochem. Biophys. Res. Commun., 425(4):775-80.
PMID: 22885184
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Chakraborty G et al. (2012), Semaphorin 3A suppresses tumor growth and metastasis in mice melanoma model. PLoS ONE, 7(3):e33633.
PMID: 22448259
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

(2012), Neuropilin 1 deficiency on CD4+Foxp3+ regulatory T cells impairs mouse melanoma growth. J. Exp. Med., 209(11):2001-16.
PMID: 23045606
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

(2012), Neuropilin 1 is expressed on thymus-derived natural regulatory T cells, but not mucosa-generated induced Foxp3+ T reg cells. J. Exp. Med., 209(10):1723.
PMID: 22966001
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Milpied P et al. (2011), IL-17-producing invariant NKT cells in lymphoid organs are recent thymic emigrants identified by neuropilin-1 expression. Blood, 118(11):2993-3002.
PMID: 21653940
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Lin FJ et al. (2010), Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development. J. Clin. Invest., 120(5):1694-707.
PMID: 20364082
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Robinson SD et al. (2009), Alphav beta3 integrin limits the contribution of neuropilin-1 to vascular endothelial growth factor-induced angiogenesis. J. Biol. Chem., 284(49):33966-81.
PMID: 19837659
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot

Marko SB et al. (2008), VEGF promotes vascular sympathetic innervation. Am. J. Physiol. Heart Circ. Physiol., 294(6):H2646-52.
PMID: 18408130
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Kaneko-Goto T et al. (2008), BIG-2 mediates olfactory axon convergence to target glomeruli. Neuron, 57(6):834-46.
PMID: 18367085
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Zacchigna S et al. (2008), Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice. J. Clin. Invest., 118(6):2062-75.
PMID: 18483621
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Chauvet S et al. (2007), Gating of Sema3E/PlexinD1 signaling by neuropilin-1 switches axonal repulsion to attraction during brain development. Neuron, 56(5):807-22.
PMID: 18054858
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Mire E et al. (2007), Modulating Sema3A signal with a L1 mimetic peptide is not sufficient to promote motor recovery and axon regeneration after spinal cord injury. Mol. Cell. Neurosci., 37(2):222-35.
PMID: 17997325
Species: Chicken
Sample Types: Whole Tissue
Applications: IHC-Fr

Moret F et al. (2007), Semaphorin and neuropilin co-expression in motoneurons sets axon sensitivity to environmental semaphorin sources during motor axon pathfinding. Development, 134(24):4491-501.
PMID: 18039974
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Fantin A et al. (0), The embryonic mouse hindbrain as a qualitative and quantitative model for studying the molecular and cellular mechanisms of angiogenesis. Nat Proto8(2):418-29.
PMID: 23424750

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Customer Reviews for Mouse/Rat Neuropilin-1 Antibody (2)

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Mouse/Rat Neuropilin-1 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: E12.5 mouse embryo fixed in 4% PFA

Species: Mouse

Verified Customer | Posted 12/03/2020

Antibody was stained on E12.5 mouse sections (attached picture) as well as E9.5 mouse sections.Worked well.Note the positive stain in the arteries and the negative signal in the adjacent veins.Dilution used - 1:20
Mouse/Rat Neuropilin-1 Antibody

Name: Alina Filatova

Application: IHC

Sample Tested: paraffin-embedded brain

Species: Mouse

Verified Customer | Posted 01/23/2019

The antibody is very sensitive to fixation and epitope retrieval conditions. Many conditions need to be tested to achieve an optimal staining. Testing several dilutions with each sample is recommended.

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Mouse/Rat Neuropilin-1 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Mouse/Rat Neuropilin-1 Antibody

Scientific Data Examples for Mouse/Rat Neuropilin-1 Antibody

Western Blot

Flow Cytometry

Immunohistochemistry

Immunohistochemistry

Immunohistochemistry

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Neuropilin-1

Product Specific Notices for Mouse/Rat Neuropilin-1 Antibody

Citations for Mouse/Rat Neuropilin-1 Antibody (50)

Customer Reviews for Mouse/Rat Neuropilin-1 Antibody (2)

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Mouse/Rat Neuropilin-1 Antibody

Mouse/Rat Neuropilin-1 Antibody

Product Documents for Mouse/Rat Neuropilin-1 Antibody

Product Datasheets

COA

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