Mouse/Rat Neuropilin-1 Antibody
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity: | Mouse, Rat |
Immunogen: |
Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1 Phe22-Ala810 (Lys811Arg), Ser829-Asp854 Accession # Q9QWJ9 |
Applications: | Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot |
Label: | Unconjugated |
Source: | Polyclonal Goat IgG |
Specificity: |
Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed. |
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Citations for Mouse/Rat Neuropilin-1 Antibody (50)
Citations are publications that use Bio-Techne products. Selected citations for Mouse/Rat Neuropilin-1 Antibody include:
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Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotFantin et al. (2104), Neuropilin 1 (NRP1) hypomorphism combined with defective VEGF-A binding reveals novel roles for NRP1 in developmental and pathological angiogenesis. Development, 141(3):556-62.
PMID: 24401374 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCEMMA Andriessen et al. (2021), Myeloid-resident neuropilin-1 promotes choroidal neovascularization while mitigating inflammation Embo Molecular Medicine, 0(0):e11754.
PMID: 33876574 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationG Limoni et al. (2021), PlexinA4-Semaphorin3A-mediated crosstalk between main cortical interneuron classes is required for superficial interneuron lamination Cell Reports, 34(4):108644.
PMID: 33503438 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationCM Termini et al. (2021), Neuropilin 1 regulates bone marrow vascular regeneration and hematopoietic reconstitution Nature Communications, 12(1):6990.
PMID: 34848712 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotJ Gonzales et al. (2020), Semaphorin 3A controls enteric neuron connectivity and is inversely associated with synapsin 1 expression in Hirschsprung disease Sci Rep, 10(1):15119.
PMID: 32934297 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotN Apóstolo et al. (2020), Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 11(1):5171.
PMID: 33057002 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCT Pemmari et al. (2020), Exposed CendR Domain in Homing Peptide Yields Skin-Targeted Therapeutic in Epidermolysis Bullosa Mol. Ther., 0(0).
PMID: 32497513 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCD Nilsson et al. (2019), Foxc2 is essential for podocyte function Physiol Rep, 7(9):e14083.
PMID: 31062503 -
Species: Mouse
Sample Types: Whole Cells
Applications: Cell CultureCR Bye et al. (2019), Axonal growth of midbrain dopamine neurons is modulated by the cell adhesion molecule ALCAM through trans-heterophilic interaction with L1Cam, ChL1 and Semaphorin J. Neurosci., 0(0).
PMID: 31300520 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotMM Hossain et al. (2019), PlexinA1 is crucial for the midline crossing of callosal axons during corpus callosum development in BALB/cAJ mice PLoS ONE, 14(8):e0221440.
PMID: 31430342 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCDM Barry et al. (2019), Molecular determinants of nephron vascular specialization in the kidney Nat Commun, 10(1):5705.
PMID: 31836710 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCEZM Taroc et al. (2019), Gli3 regulates vomeronasal neurogenesis, olfactory ensheathing cell formation and GnRH-1 neuronal migration J. Neurosci., 0(0).
PMID: 31767679 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCAM Wilson et al. (2018), Neuropilin-1 expression in adipose tissue macrophages protects against obesity and metabolic syndrome Sci Immunol, 3(21).
PMID: 29549139 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrN Yang et al. (2017), Suppression of Retinal Neovascularization by Inhibition of Galectin-1 in a Murine Model of Oxygen-Induced Retinopathy J Ophthalmol, 2017(0):5053035.
PMID: 28428895 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCN Yang et al. (2017), Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy Exp. Eye Res, 0(0).
PMID: 28257831 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCH Lee et al. (2017), Rewiring the taste system Nature, 548(7667):330-333.
PMID: 28792937 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrK Inokuchi et al. (2017), Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odour-induced attractive social responses Nat Commun, 8(0):15977.
PMID: 28731029 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCP Fern ndez- et al. (2017), Neuropilin 1 Involvement in Choroidal and Retinal Neovascularisation PLoS ONE, 12(1):e0169865.
PMID: 28107458 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCJM Pinskey et al. (2017), Neuropilin-1 promotes Hedgehog signaling through a novel cytoplasmic motif J. Biol. Chem., 0(0).
PMID: 28667171 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCPeter Mombaerts (2016), Neuropilin-1 and the Positions of Glomeruli in the Mouse Olfactory Bulb eNeuro, 3(5).
PMID: 27844052 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC(2016), NOVA2-mediated RNA regulation is required for axonal pathfinding during development Elife, 5(0).
PMID: 27223325 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCRama N et al. (2015), Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization. Nat Med, 21(5):483-91.
PMID: 25894826 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCPlein A et al. (2015), Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation. J Clin Invest, 125(7):2661-76.
PMID: 26053665 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCTillo M et al. (2015), VEGF189 binds NRP1 and is sufficient for VEGF/NRP1-dependent neuronal patterning in the developing brain. Development, 142(2):314-9.
PMID: 25519242 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCGiacobini P et al. (2014), Brain endothelial cells control fertility through ovarian-steroid-dependent release of semaphorin 3A. PLoS Biol, 12(3):e1001808.
PMID: 24618750 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometrySmolarchuk C et al. (2014), T cells generated in the absence of a thoracic thymus fail to establish homeostasis. Eur J Immunol, 44(8):2263-73.
PMID: 24777700 -
Species: Mouse
Sample Types: Whole Tissue
Applications: Functional AssayEsquibies A et al. (2014), Heparin binding VEGF isoforms attenuate hyperoxic embryonic lung growth retardation via a FLK1-neuropilin-1-PKC dependent pathway. Respir Res, 15(0):32.
PMID: 24641672 -
Species: Rat
Sample Types: Whole Cells
Applications: NeutralizationMolofsky A et al. (2014), Astrocyte-encoded positional cues maintain sensorimotor circuit integrity. Nature, 509(7499):189-94.
PMID: 24776795 -
Miyazaki et al. (2014), Id2 and Id3 maintain the regulatory T cell pool to suppress inflammatory disease. Nat Immunol, 15(8):767-76.
PMID: 24973820 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotShahrabi-Farahani et al. (2014), Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas. Lab Invest, 94(7):752-65.
PMID: 24791743 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCLee et al. (2014), Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing. Nat Immunol, 15(10):982-95.
PMID: 25173345 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotRaimondi C et al. (2014), Imatinib inhibits VEGF-independent angiogenesis by targeting neuropilin 1-dependent ABL1 activation in endothelial cells. J Exp Med, 211(6):1167-83.
PMID: 24863063 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryPetzold C et al. (2014), Fluorochrome-based definition of naturally occurring Foxp3(+) regulatory T cells of intra- and extrathymic origin. Eur J Immunol, 44(12):3632-45.
PMID: 25159127 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-PEzkurdia N et al. (2014), Inhibition of neuronal apoptosis and axonal regression ameliorates sympathetic atrophy and hemodynamic alterations in portal hypertensive rats. PLoS ONE, 9(1):e84374.
PMID: 24400086 -
Species: Mouse
Sample Types: Cell Lysates
Applications: ImmunoprecipitationDelgoffe et al. (2013), Stability and function of regulatory T cells is maintained by a neuropilin-1-semaphorin-4a axis. Nature, 501(7466):252-6.
PMID: 23913274 -
Species: Human
Sample Types: Whole Cells
Applications: Neutralizationvan Gils et al. (2013), Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions. Arterioscler Thromb Vasc Biol, 33(5):911-9.
PMID: 23430612 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryGlinka Y et al. (2012), Neuropilin-1 is expressed by breast cancer stem-like cells and is linked to NF-kappaB activation and tumor sphere formation. Biochem. Biophys. Res. Commun., 425(4):775-80.
PMID: 22885184 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationChakraborty G et al. (2012), Semaphorin 3A suppresses tumor growth and metastasis in mice melanoma model. PLoS ONE, 7(3):e33633.
PMID: 22448259 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry(2012), Neuropilin 1 deficiency on CD4+Foxp3+ regulatory T cells impairs mouse melanoma growth. J. Exp. Med., 209(11):2001-16.
PMID: 23045606 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry(2012), Neuropilin 1 is expressed on thymus-derived natural regulatory T cells, but not mucosa-generated induced Foxp3+ T reg cells. J. Exp. Med., 209(10):1723.
PMID: 22966001 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryMilpied P et al. (2011), IL-17-producing invariant NKT cells in lymphoid organs are recent thymic emigrants identified by neuropilin-1 expression. Blood, 118(11):2993-3002.
PMID: 21653940 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PLin FJ et al. (2010), Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development. J. Clin. Invest., 120(5):1694-707.
PMID: 20364082 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotRobinson SD et al. (2009), Alphav beta3 integrin limits the contribution of neuropilin-1 to vascular endothelial growth factor-induced angiogenesis. J. Biol. Chem., 284(49):33966-81.
PMID: 19837659 -
Species: Rat
Sample Types: Cell Lysates
Applications: Western BlotMarko SB et al. (2008), VEGF promotes vascular sympathetic innervation. Am. J. Physiol. Heart Circ. Physiol., 294(6):H2646-52.
PMID: 18408130 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotKaneko-Goto T et al. (2008), BIG-2 mediates olfactory axon convergence to target glomeruli. Neuron, 57(6):834-46.
PMID: 18367085 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrZacchigna S et al. (2008), Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice. J. Clin. Invest., 118(6):2062-75.
PMID: 18483621 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCChauvet S et al. (2007), Gating of Sema3E/PlexinD1 signaling by neuropilin-1 switches axonal repulsion to attraction during brain development. Neuron, 56(5):807-22.
PMID: 18054858 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCMire E et al. (2007), Modulating Sema3A signal with a L1 mimetic peptide is not sufficient to promote motor recovery and axon regeneration after spinal cord injury. Mol. Cell. Neurosci., 37(2):222-35.
PMID: 17997325 -
Species: Chicken
Sample Types: Whole Tissue
Applications: IHC-FrMoret F et al. (2007), Semaphorin and neuropilin co-expression in motoneurons sets axon sensitivity to environmental semaphorin sources during motor axon pathfinding. Development, 134(24):4491-501.
PMID: 18039974 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCFantin A et al. (0), The embryonic mouse hindbrain as a qualitative and quantitative model for studying the molecular and cellular mechanisms of angiogenesis. Nat Proto8(2):418-29.
PMID: 23424750
There are no citations that match your criteria.
Customer Reviews for Mouse/Rat Neuropilin-1 Antibody (2)
Customer Images
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Application: ICC/IFSample Tested: E12.5 mouse embryo fixed in 4% PFASpecies: MouseVerified Customer | Posted 12/03/2020Antibody was stained on E12.5 mouse sections (attached picture) as well as E9.5 mouse sections.Worked well.Note the positive stain in the arteries and the negative signal in the adjacent veins.Dilution used - 1:20
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Application: IHCSample Tested: paraffin-embedded brainSpecies: MouseVerified Customer | Posted 01/23/2019The antibody is very sensitive to fixation and epitope retrieval conditions. Many conditions need to be tested to achieve an optimal staining. Testing several dilutions with each sample is recommended.
There are no reviews that match your criteria.