Recombinant Human Neuropilin-1 Protein, CF

Name: Recombinant Human Neuropilin-1 Protein, CF
Brand: R&D Systems
SKU: 3870-N1
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3870-N1

Citations (11)

(1)

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Source:	NS0
Accession #:	NP_001019799
Applications:	Binding Activity

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Product Specifications for Recombinant Human Neuropilin-1 Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Neuropilin-1 protein Phe22-Lys644, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Phe22
Predicted Molecular Mass	70.6 kDa
SDS-PAGE	85-95 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Neuropilin-1 can bind recombinant human VEGF₁₆₅ with an apparent K_d <1 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3870-N1

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuropilin-1

Neuropilin-1 protein (Nrp1, previously neuropilin; also CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1 - 4). The full-length 923 amino acid (aa) human NRP1 contains a 623 aa extracellular domain (ECD) that shows 92 - 95% aa identity with mouse, rat, bovine and canine NRP1 (3, 4). The NRP1 ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). C-terminally divergent splice variants with 704, 644, 609, and 551 aa lack the MAM and TM domains and are demonstrated or presumed to be soluble antagonists (1, 5 - 7). A 906 aa form lacks a TM segment, but secretion has not been found (8). The sema domains of Class III secreted semaphorins such as Sema3A bind NRP1 a1a2 (9). Heparin, the heparin-binding forms of VEGF (VEGF165, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the b1b2 region (9, 10). NRP1 and NRP2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (11). However, only NRP1 binds Sema3A, and only NRP2 binds Sema3F (1). Both are co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF165 binding (1). Sema3A signaling can be blocked by VEGF165, which has higher affinity for NRP1 (12). NRP1 is preferentially expressed in arteries during development or those undergoing remodeling (1, 2). NRP1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (13).

Neuropilin-1 has been shown to bind to the S1 domain of the SARS-CoV-2 Spike protein via its b1 domain (14,15). Blocking this interaction reduced viral entry and infectivity in vitro providing a new potential therapeutic target to treat COVID-19.

Item	Value
References	Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584. Gu, C. et al. (2003) Dev. Cell 5:45. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739. Soker, S. et al. (1998) Cell 92:735. Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573. Cackowski, F.C. et al. (2004) Genomics 84:82. Rossignol, M. et al. (2000) Genomics 70:211. Tao, Q. et al. (2003) Angiogenesis 6:39. Gu, C. et al. (2002) J. Biol. Chem. 277:18069. Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818. Yaron, A. et al. (2005) Neuron 45:513. Narazaki, M. and G. Tosato (2006) Blood 107:3892. Tordjman, R. et al. (2002) Nat. Immunol. 3477. Daly, J.L. et al. (2020) Science. Oct 20:eabd3072. doi: 10.1126/science.abd3072. Cantuti-Castelvetri L. et al. (2020) Science. Oct 20:eabd2985. doi: 10.1126/science.abd2985.
Entrez Gene IDs	8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names	BDCA-4, BDCA4, CD304, CD304 antigen, DKFZp686A03134, DKFZp781F1414, NP1, NRP, NRP1, Neuropilin-1, Neuropilin1, VEGF165R, Vascular endothelial cell growth factor 165 receptor, neuropilin 1, transmembrane receptor

Citations for Recombinant Human Neuropilin-1 Protein, CF (11)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Neuropilin-1 Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

L Matilla et al. (2020), Soluble St2 Induces Cardiac Fibroblast Activation and Collagen Synthesis via Neuropilin-1 Cells, 9(7).
PMID: 32664340
Species: Human
Sample Types: Protein
Applications: SPR

L Lu et al. (2020), A Dual Receptor Targeting- and BBB Penetrating- Peptide Functionalized Polyethyleneimine Nanocomplex for Secretory Endostatin Gene Delivery to Malignant Glioma Int J Nanomedicine, 15(0):8875-8892.
PMID: 33209022
Species: Vicugna pacos (alpaca)
Sample Types: In Vivo
Applications: In Vivo

Y De Vlaemin et al. (2020), Targeting Neuropilin-1 with Nanobodies Reduces Colorectal Carcinoma Development Cancers, 12(12).
PMID: 33266104
Species: Human
Sample Types: Recombinant Protein
Applications: Direct ELISA

JR Podojil et al. (2018), B7-H4 Modulates Regulatory CD4+ T Cell Induction and Function via Ligation of a Semaphorin 3a/Plexin A4/Neuropilin-1 Complex J. Immunol., 0(0).
PMID: 29898965
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay

C Kerros et al. (2017), Neuropilin-1 mediates Neutrophil Elastase uptake and cross-presentation in breast cancer cells J. Biol. Chem., 0(0).
PMID: 28468826
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Teran M et al. (2015), Synergistic Binding of Vascular Endothelial Growth Factor-A and Its Receptors to Heparin Selectively Modulates Complex Affinity. J Biol Chem, 290(26):16451-62.
PMID: 25979342
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 487(7408):505-9.
PMID: 22763448
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Uniewicz KA et al. (2011), Exogenous recombinant dimeric neuropilin-1 is sufficient to drive angiogenesis. J. Biol. Chem., 286(1):12-23.
PMID: 20956519
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay

Narazaki M et al. (2010), Oligo-guanosine nucleotide induces neuropilin-1 internalization in endothelial cells and inhibits angiogenesis. Blood, 116(16):3099-107.
PMID: 20606164
Species: Mouse
Sample Types: Protein
Applications: Bioassay

Jaalouk DE et al. (2007), The original Pathologische Anatomie Leiden-Endothelium monoclonal antibody recognizes a vascular endothelial growth factor binding site within neuropilin-1. Cancer Res., 67(20):9623-9.
PMID: 17942890
Species: Virus
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR

Martinez-Martin N et al. (0), An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor. Cel174(5):1158-1171.e19.
PMID: 30057110

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Recombinant Human Neuropilin-1 Protein, CF

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Application: In vitro bioactivity in cell culture

Verified Customer | Posted 08/21/2019

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Recombinant Human Neuropilin-1 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human Neuropilin-1 Protein, CF

Formulation, Preparation and Storage

Carrier Free

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Background: Neuropilin-1

Citations for Recombinant Human Neuropilin-1 Protein, CF (11)

Customer Reviews for Recombinant Human Neuropilin-1 Protein, CF (1)

Recombinant Human Neuropilin-1 Protein, CF

Product Documents for Recombinant Human Neuropilin-1 Protein, CF

Product Datasheets

COA

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