Recombinant Mouse Neuropilin-1 Protein, CF

Name: Recombinant Mouse Neuropilin-1 Protein, CF
Brand: R&D Systems
SKU: 5994-N1
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 5994-N1

Citations (4)

(1)

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Key Product Details

Source:	NS0
Accession #:	NP_032763
Structure / Form:	Noncovalently-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Neuropilin-1 Protein, CF

Product Specifications for Recombinant Mouse Neuropilin-1 Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse Neuropilin-1 protein Phe22-Pro856, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Phe22
Predicted Molecular Mass	94.5 kDa (monomer)
SDS-PAGE	100-120 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse Neuropilin‑1 can bind recombinant human VEGF₁₆₅ with an apparent K_D <1 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5994-N1

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuropilin-1

Neuropilin-1 (Npn-1, previously neuropilin; also CD304 or BDCA4 in humans) is a 130‑140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1‑4). The full-length 923 amino acid (aa) mouse Npn-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with rat and 93% aa identity with human, equine, bovine and canine Npn-1 (3, 4). The ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). At least one splice variant that diverges at aa 587 and lacks the TM domain has been sequenced (5). This form is potentially a soluble antagonist, based on results from human Npn-1 splice variants (1, 6‑8). The sema domains of Class III secreted semaphorins such as Sema3A bind Npn-1 a1a2 (9). Heparin, the heparin-binding forms of VEGF (VEGF₁₆₅, VEGF-B and VEGF-E), PlGF (PlGF2), and the C‑terminus of Sema3 bind the b1b2 region (9, 10). Npn-1 and Npn-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both Neuropilins act as co-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (11). However, only Npn-1 binds Sema3A, and only Npn-2 binds Sema3F (1). Both are co‑receptors with VEGF R2 (also called KDR or Flk-1) for VEGF₁₆₅ binding (1). Sema3A signaling can be blocked by VEGF₁₆₅, which has higher affinity for Npn-1 (12). Npn-1 is preferentially expressed in developing or remodeling arteries (1, 2). Npn-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (13).

Item	Value
References	Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584. Gu, C. et al. (2003) Dev. Cell 5:45. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739. Soker, S. et al. (1998) Cell 92:735. Entrez Accession # EDL11827. Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573. Cackowski, F.C. et al. (2004) Genomics 84:82. Rossignol, M. et al. (2000) Genomics 70:211. Gu, C. et al. (2002) J. Biol. Chem. 277:18069. Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818. Yaron, A. et al. (2005) Neuron 45:513. Narazaki, M. and G. Tosato (2006) Blood 107:3892. Tordjman, R. et al. (2002) Nat. Immunol. 34:77.
Entrez Gene IDs	8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names	BDCA-4, BDCA4, CD304, CD304 antigen, DKFZp686A03134, DKFZp781F1414, NP1, NRP, NRP1, Neuropilin-1, Neuropilin1, VEGF165R, Vascular endothelial cell growth factor 165 receptor, neuropilin 1, transmembrane receptor

Product Specific Notices for Recombinant Mouse Neuropilin-1 Protein, CF

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

Citations for Recombinant Mouse Neuropilin-1 Protein, CF (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Neuropilin-1 Protein, CF include:

Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay

L Muhl et al. (2017), Neuropilin 1 binds platelet-derived growth factor (PDGF)-D and is a co-receptor in PDGF-D/PDGF receptor ? signaling J. Cell. Sci, 0(0).
PMID: 28254885
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Teran M et al. (2015), Synergistic Binding of Vascular Endothelial Growth Factor-A and Its Receptors to Heparin Selectively Modulates Complex Affinity. J Biol Chem, 290(26):16451-62.
PMID: 25979342
Species: N/A
Sample Types: N/A
Applications: Binding Assay

Delgoffe et al. (2013), Stability and function of regulatory T cells is maintained by a neuropilin-1-semaphorin-4a axis. Nature, 501(7466):252-6.
PMID: 23913274
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Hayashi M et al. (2012), Osteoprotection by semaphorin 3A. Nature, 485(7396):69-74.
PMID: 22522930

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Customer Reviews for Recombinant Mouse Neuropilin-1 Protein, CF (1)

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Recombinant Mouse Neuropilin-1 Protein, CF

Name: Dongmei liu

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 05/14/2016

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Product Documents for Recombinant Mouse Neuropilin-1 Protein, CF

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Recombinant Mouse Neuropilin-1 Protein, CF

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Product Specifications for Recombinant Mouse Neuropilin-1 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Neuropilin-1

Product Specific Notices for Recombinant Mouse Neuropilin-1 Protein, CF

Citations for Recombinant Mouse Neuropilin-1 Protein, CF (4)

Customer Reviews for Recombinant Mouse Neuropilin-1 Protein, CF (1)

Recombinant Mouse Neuropilin-1 Protein, CF

Product Documents for Recombinant Mouse Neuropilin-1 Protein, CF

Product Datasheets

COA

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SDS

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