Mouse/Rat Neuropilin-1 PE-conjugated Antibody

Name: Mouse/Rat Neuropilin-1 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB566P
Price: 392 EUR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB566P

Citations (1)

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Key Product Details

Species Reactivity:	Mouse, Rat
Immunogen:	Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1 Phe22-Ala810 (Lys811Arg), Ser829-Asp854 Accession # Q9QWJ9
Applications:	Flow Cytometry
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 8% cross-reactivity with recombinant human Neuropilin‑1 is observed, and less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed.

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Applications for Mouse/Rat Neuropilin-1 PE-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse/Rat Neuropilin-1 PE-conjugated Antibody

	Flow Cytometry Detection of Neuropilin‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse/Rat Neuropilin-1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.
	Flow Cytometry Detection of Neuropilin‑1 in PC‑12 Rat Cell Line by Flow Cytometry. PC-12 rat adrenal pheochromocytoma cell line was stained with Goat Anti-Mouse/Rat Neuropilin-1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: Neuropilin-1

Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. The amino acid sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.

Item	Value
Entrez Gene IDs	8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names	BDCA-4, BDCA4, CD304, CD304 antigen, DKFZp686A03134, DKFZp781F1414, NP1, NRP, NRP1, Neuropilin-1, Neuropilin1, VEGF165R, Vascular endothelial cell growth factor 165 receptor, neuropilin 1, transmembrane receptor

Product Specific Notices for Mouse/Rat Neuropilin-1 PE-conjugated Antibody

* This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

Citations for Mouse/Rat Neuropilin-1 PE-conjugated Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse/Rat Neuropilin-1 PE-conjugated Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Imoukhuede et al. (2012), Expression of VEGF receptors on endothelial cells in mouse skeletal muscle. PLoS ONE, 7(9):e44791.
PMID: 22984559

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