Rat Neuropilin-1 Biotinylated Antibody

Name: Rat Neuropilin-1 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF566
Price: 515 EUR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF566

Citations (7)

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Species Reactivity:	Rat
Immunogen:	Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1 Phe22-Ala810,Arg,Ser829-Asp854 Accession # Q9QWJ9
Applications:	Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects rat Neuropilin-1 extracellular domain in Western blots. In this format, less than 1% cross-reactivity with recombinant rat Neuropilin‑2 is observed.

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Applications for Rat Neuropilin-1 Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Rat Neuropilin‑1/BDCA4 Fc Chimera (Catalog # 566-N1)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Neuropilin-1

Neuropilin-1 (Npn-1, previously neuropilin; also CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1 - 4). The mature 901 amino acid (aa) rat Npn-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with mouse and 93% aa identity with human, equine, bovine and canine Npn-1 (3, 4). The ECD contains two N-terminal CUB domains, two F5/8 type C domains with homology to coagulation factors V and VIII and a MAM (meprin) domain. In mouse and human, splice variants that lack the TM domain have been described and are either proven or presumed to be soluble antagonists (1, 5 - 7). The sema domains of Class III secreted semaphorins such as Sema3A bind Npn-1 CUB domains (8). The heparin-binding forms of VEGF (VEGF₁₆₅, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the F5/8 type C domains (8, 9). Npn-1 and Npn-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (10). However, only Npn-1 binds Sema3A, and only Npn-2 binds Sema3F (1). Both are co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF₁₆₅ binding (1). Sema3A signaling can be blocked by VEGF₁₆₅, which has higher affinity for Npn-1 (11). Npn-1 is preferentially expressed in developing or remodeling arteries (1, 2). Npn-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (12).

Item	Value
References	Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584. Gu, C. et al. (2003) Dev. Cell 5:45. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739. Soker, S. et al. (1998) Cell 92:735. Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573. Cackowski, F.C. et al. (2004) Genomics 84:82. Rossignol, M. et al. (2000) Genomics 70:211. Gu, C. et al. (2002) J. Biol. Chem. 277:18069. Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818. Yaron, A. et al. (2005) Neuron 45:513. Narazaki, M. and G. Tosato (2006) Blood 107:3892. Tordjman, R. et al. (2002) Nat. Immunol. 3477.
Entrez Gene IDs	8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names	BDCA-4, BDCA4, CD304, CD304 antigen, DKFZp686A03134, DKFZp781F1414, NP1, NRP, NRP1, Neuropilin-1, Neuropilin1, VEGF165R, Vascular endothelial cell growth factor 165 receptor, neuropilin 1, transmembrane receptor

Product Specific Notices for Rat Neuropilin-1 Biotinylated Antibody

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to >obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

Citations for Rat Neuropilin-1 Biotinylated Antibody (7)

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Citations are publications that use Bio-Techne products. Selected citations for Rat Neuropilin-1 Biotinylated Antibody include:

Species: Mouse

D Esterházy et al. (2019), Compartmentalized gut lymph node drainage dictates adaptive immune responses Nature, 0(0).
PMID: 30988509
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Z Zhang et al. (2017), Activation and Functional Specialization of Regulatory T Cells Lead to the Generation of Foxp3 Instability J. Immunol, 0(0).
PMID: 28228556
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

X Wei et al. (2017), Reciprocal Expression of IL-35 and IL-10 Defines Two Distinct Effector Treg Subsets that Are Required for Maintenance of Immune Tolerance Cell Rep, 21(7):1853-1869.
PMID: 29141218
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Yao Y et al. (2015), Tr1 Cells, but Not Foxp3+ Regulatory T Cells, Suppress NLRP3 Inflammasome Activation via an IL-10-Dependent Mechanism. J Immunol, 195(2):488-97.
PMID: 26056255
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Nystrom S et al. (2014), Transient Treg-cell depletion in adult mice results in persistent self-reactive CD4(+) T-cell responses. Eur J Immunol, 44(12):3621-31.
PMID: 25231532
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot

Romi E et al. (2014), ADAM metalloproteases promote a developmental switch in responsiveness to the axonal repellant Sema3A. Nat Commun, 5(0):4058.
PMID: 24898499
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

(2012), Neuropilin 1 is expressed on thymus-derived natural regulatory T cells, but not mucosa-generated induced Foxp3+ T reg cells. J. Exp. Med., 209(10):1723.
PMID: 22966001

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Showing 1 - 7 of 7 citations Showing All

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Rat Neuropilin-1 Biotinylated Antibody

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