Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF

Name: Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 566-N1
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 566-N1

Citations (5)

(1)

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Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	Q9QWJ9
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

View Full Technical Details for Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF

Product Specifications for Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat Neuropilin-1 protein

Rat Neuropilin-1 Phe22 - Asp854 (Lys811Arg and Pro812 - Gly828 del) Accession # Q9QWJ9	IEGRDMD	Human IgG₁ (Pro100 - Lys330)	6-His tag
N-terminus			C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.1 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Phe22

Predicted Molecular Mass

119 kDa (monomer)

SDS-PAGE

130-150 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Immobilized rrNeuropilin-1/Fc Chimera can bind rhVEGF₁₆₅ with an apparent K_D < 1 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

566-N1

Formulation	Lyophilized from a 0.2 μm filtered solution in Citrate Phosphate and NaCl.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuropilin-1

Neuropilin-1 (Npn-1, previously neuropilin; also CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1 - 4). The mature 901 amino acid (aa) rat Npn-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with mouse and 93% aa identity with human, equine, bovine and canine Npn-1 (3, 4). The ECD contains two N-terminal CUB domains, two F5/8 type C domains with homology to coagulation factors V and VIII and a MAM (meprin) domain. In mouse and human, splice variants that lack the TM domain have been described and are either proven or presumed to be soluble antagonists (1, 5 - 7). The sema domains of Class III secreted semaphorins such as Sema3A bind Npn-1 CUB domains (8). The heparin-binding forms of VEGF (VEGF₁₆₅, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the F5/8 type C domains (8, 9). Npn-1 and Npn-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (10). However, only Npn-1 binds Sema3A, and only Npn-2 binds Sema3F (1). Both are co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF₁₆₅ binding (1). Sema3A signaling can be blocked by VEGF₁₆₅, which has higher affinity for Npn-1 (11). Npn-1 is preferentially expressed in developing or remodeling arteries (1, 2). Npn-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (12).

Item	Value
References	Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584. Gu, C. et al. (2003) Dev. Cell 5:45. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739. Soker, S. et al. (1998) Cell 92:735. Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573. Cackowski, F.C. et al. (2004) Genomics 84:82. Rossignol, M. et al. (2000) Genomics 70:211. Gu, C. et al. (2002) J. Biol. Chem. 277:18069. Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818. Yaron, A. et al. (2005) Neuron 45:513. Narazaki, M. and G. Tosato (2006) Blood 107:3892. Tordjman, R. et al. (2002) Nat. Immunol. 3477.
Entrez Gene IDs	8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names	BDCA-4, BDCA4, CD304, CD304 antigen, DKFZp686A03134, DKFZp781F1414, NP1, NRP, NRP1, Neuropilin-1, Neuropilin1, VEGF165R, Vascular endothelial cell growth factor 165 receptor, neuropilin 1, transmembrane receptor

Product Specific Notices for Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

Citations for Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF include:

Species: Rat
Sample Types: Protein
Applications: ELISA (Capture)

D Tymecka et al. (2017), Structure-activity relationship study of tetrapeptide inhibitors of the Vascular Endothelial Growth Factor A binding to Neuropilin-1 Peptides, 0(0).
PMID: 28627371
Species: Human
Sample Types: Protein
Applications: Bioassay

Teran M et al. (2015), Synergistic Binding of Vascular Endothelial Growth Factor-A and Its Receptors to Heparin Selectively Modulates Complex Affinity. J Biol Chem, 290(26):16451-62.
PMID: 25979342
Species: Human
Sample Types: Buffer
Applications: Bioassay

Lake AC et al. (2006), Low molecular weight fucoidan increases VEGF165-induced endothelial cell migration by enhancing VEGF165 binding to VEGFR-2 and NRP1. J. Biol. Chem., 281(49):37844-52.
PMID: 17028197
Species: Virus
Sample Types: Recombinant Protein
Applications: Binding Assay

Tokunaga Y et al. (2006), Localization of heparin- and neuropilin-1-recognition sites of viral VEGFs. Biochem. Biophys. Res. Commun., 348(3):957-62.
PMID: 16899214
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Narazaki M et al. (2006), Ligand-induced internalization selects use of common receptor neuropilin-1 by VEGF165 and semaphorin3A. Blood, 107(10):3892-901.
PMID: 16424390

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Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF

Name: Rahul Mallick

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 08/17/2020

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Product Documents for Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF

Product Datasheets

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Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Neuropilin-1

Product Specific Notices for Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF

Citations for Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF (5)

Customer Reviews for Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF (1)

Customer Images

Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF

Product Documents for Recombinant Rat Neuropilin-1 Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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