Recombinant Rat Neuropilin-1 Fc Chimera (NS0-expressed), CF

Name: Recombinant Rat Neuropilin-1 Fc Chimera (NS0-expressed), CF
Brand: R&D Systems
SKU: 566-NNS
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 566-NNS

Citations (3)

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Key Product Details

Source:	NS0
Accession #:	Q9QWJ9
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

View Full Technical Details for Recombinant Rat Neuropilin-1 Fc Chimera (NS0-expressed), CF

Product Specifications for Recombinant Rat Neuropilin-1 Fc Chimera (NS0-expressed), CF

Source

Mouse myeloma cell line, NS0-derived rat Neuropilin-1 protein

Rat Neuropilin-1 (Phe22 - Ala810) Accession # Q9QWJ9	R	Rat Neuropilin-1 (Ser829 - Asp854) Accession # Q9QWJ9	IEGRDMD	Human IgG₁ (Pro100 - Lys330)	6-His tag
N-terminus					C-terminus

R&D Systems rat Npn-1 may represent a previously unreported splice isoform of rat Npn-1.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Phe22

Predicted Molecular Mass

119 kDa (monomer)

SDS-PAGE

130-150 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Immobilized rrNpn-1/Fc Chimera at 2 µg/mL (100 µL/well) can bind rhVEGF₁₆₅ in the presence of 2 µg/mL of heparin with an apparent K_D <1 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

566-NNS

Formulation	Lyophilized from a 0.2 μm filtered solution in Citrate Phosphate and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuropilin-1

Neuropilin-1 (Npn-1, previously neuropilin; also named CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1 - 4). The full-length 922 amino acid (aa) rat Npn-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with mouse and 93% aa identity with human, equine, bovine and canine Npn-1 (3, 4). The ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). In mouse and human, splice variants that lack the TM domain have been described and are either proven or presumed to be soluble antagonists (1, 5 - 7). The sema domains of Class III secreted semaphorins such as Sema3A bind Npn-1 a1a2 (8). Heparin, the heparin-binding forms of VEGF (VEGF₁₆₅, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the b1b2 region (8, 9). Npn-1 and Npn-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act asco-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (10). However, only Npn-1 binds Sema3A, and only Npn-2 binds Sema3F (1). Both areco-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF₁₆₅ binding (1). Sema3A signaling can be blocked by VEGF₁₆₅, which has higher affinity for Npn-1 (11). Npn-1 is preferentially expressed in developing or remodeling arteries (1, 2). Npn-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (12).

Item	Value
References	Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584. Gu, C. et al. (2003) Dev. Cell 5:45. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739. Soker, S. et al. (1998) Cell 92:735. Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573. Cackowski, F.C. et al. (2004) Genomics 84:82. Rossignol, M. et al. (2000) Genomics 70:211. Gu, C. et al. (2002) J. Biol. Chem. 277:18069. Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818. Yaron, A. et al. (2005) Neuron 45:513. Narazaki, M. and G. Tosato (2006) Blood 107:3892. Tordjman, R. et al. (2002) Nat. Immunol. 3477.
Entrez Gene IDs	8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names	BDCA-4, BDCA4, CD304, CD304 antigen, DKFZp686A03134, DKFZp781F1414, NP1, NRP, NRP1, Neuropilin-1, Neuropilin1, VEGF165R, Vascular endothelial cell growth factor 165 receptor, neuropilin 1, transmembrane receptor

Product Specific Notices for Recombinant Rat Neuropilin-1 Fc Chimera (NS0-expressed), CF

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

Citations for Recombinant Rat Neuropilin-1 Fc Chimera (NS0-expressed), CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat Neuropilin-1 Fc Chimera (NS0-expressed), CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Uniewicz KA et al. (2011), Exogenous recombinant dimeric neuropilin-1 is sufficient to drive angiogenesis. J. Biol. Chem., 286(1):12-23.
PMID: 20956519
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay

Narazaki M et al. (2008), Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A. Blood, 111(8):4126-36.
PMID: 18272814
Species: Chicken
Sample Types: In Vivo
Applications: In Vivo

McLennan R et al. (2007), In vivo analysis reveals a critical role for neuropilin-1 in cranial neural crest cell migration in chick. Dev. Biol., 301(1):227-39.
PMID: 16959234

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Recombinant Rat Neuropilin-1 Fc Chimera (NS0-expressed), CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Rat Neuropilin-1 Fc Chimera (NS0-expressed), CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Neuropilin-1

Product Specific Notices for Recombinant Rat Neuropilin-1 Fc Chimera (NS0-expressed), CF

Citations for Recombinant Rat Neuropilin-1 Fc Chimera (NS0-expressed), CF (3)

Product Documents for Recombinant Rat Neuropilin-1 Fc Chimera (NS0-expressed), CF

Product Datasheets

COA

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SDS

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